Cloned (Comment) | Organism |
---|---|
expressed in Escherichia coli BL21(DE3) cells | Aspergillus terreus |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
1.08 | - |
4-methoxybenzyl alcohol | recombinant enzyme, at pH 6.0 and 30°C | Aspergillus terreus | |
2.88 | - |
3,4-dimethoxybenzyl alcohol | recombinant enzyme, at pH 6.0 and 30°C | Aspergillus terreus | |
4.91 | - |
3-Methoxybenzyl alcohol | recombinant enzyme, at pH 6.0 and 30°C | Aspergillus terreus | |
10.8 | - |
benzyl alcohol | recombinant enzyme, at pH 6.0 and 30°C | Aspergillus terreus |
Localization | Comment | Organism | GeneOntology No. | Textmining |
---|---|---|---|---|
microsome | - |
Aspergillus terreus | - |
- |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
74000 | - |
- |
Aspergillus terreus |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Aspergillus terreus | I7FDJ2 | - |
- |
Aspergillus terreus MTCC6324 | I7FDJ2 | - |
- |
Purification (Comment) | Organism |
---|---|
nickel Sepharose 6 column chromatography | Aspergillus terreus |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
3,4-dimethoxybenzyl alcohol + O2 | i.e. veratryl alcohol | Aspergillus terreus | 3,4-dimethoxybenzaldehyde + H2O2 | - |
? | |
3,4-dimethoxybenzyl alcohol + O2 | i.e. veratryl alcohol | Aspergillus terreus MTCC6324 | 3,4-dimethoxybenzaldehyde + H2O2 | - |
? | |
3-methoxybenzyl alcohol + O2 | i.e. 3-anisyl alcohol | Aspergillus terreus | 3-methoxybenzaldehyde + H2O2 | - |
? | |
3-methoxybenzyl alcohol + O2 | i.e. 3-anisyl alcohol | Aspergillus terreus MTCC6324 | 3-methoxybenzaldehyde + H2O2 | - |
? | |
4-methoxybenzyl alcohol + O2 | i.e. 4-anisyl alcohol, best substrate | Aspergillus terreus | 4-methoxybenzaldehyde + H2O2 | - |
? | |
4-methoxybenzyl alcohol + O2 | i.e. 4-anisyl alcohol, best substrate | Aspergillus terreus MTCC6324 | 4-methoxybenzaldehyde + H2O2 | - |
? | |
benzyl alcohol + O2 | poorest substrate | Aspergillus terreus | benzaldehyde + H2O2 | - |
? | |
benzyl alcohol + O2 | poorest substrate | Aspergillus terreus MTCC6324 | benzaldehyde + H2O2 | - |
? |
Subunits | Comment | Organism |
---|---|---|
? | x * 76000, SDS-PAGE | Aspergillus terreus |
? | x * 74614, MALDI-TOF mass spectrometry | Aspergillus terreus |
Synonyms | Comment | Organism |
---|---|---|
alcohol: O2 oxidoreductase | - |
Aspergillus terreus |
AOX | - |
Aspergillus terreus |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
30 | - |
- |
Aspergillus terreus |
Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|
30 | 70 | the enzyme is stable up to 30°C and the stability is greatly reduced at 40°C (50% inactivation) and leads to 100% inactivation after 5 min at 70°C | Aspergillus terreus |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
2.2 | - |
benzyl alcohol | recombinant enzyme, at pH 6.0 and 30°C | Aspergillus terreus | |
105.7 | - |
3,4-dimethoxybenzyl alcohol | recombinant enzyme, at pH 6.0 and 30°C | Aspergillus terreus | |
115.4 | - |
3-Methoxybenzyl alcohol | recombinant enzyme, at pH 6.0 and 30°C | Aspergillus terreus | |
140.9 | - |
4-methoxybenzyl alcohol | recombinant enzyme, at pH 6.0 and 30°C | Aspergillus terreus |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
6 | - |
- |
Aspergillus terreus |
pH Stability | pH Stability Maximum | Comment | Organism |
---|---|---|---|
6 | 9 | the recombinant enzyme demonstrates partial pH stability in range from pH 6.0 to pH 9.0 after 1 h | Aspergillus terreus |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
FAD | required for activity | Aspergillus terreus |
Organism | Comment | pI Value Maximum | pI Value |
---|---|---|---|
Aspergillus terreus | isoelectric focusing | - |
6.5 |
kcat/KM Value [1/mMs-1] | kcat/KM Value Maximum [1/mMs-1] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.2 | - |
benzyl alcohol | recombinant enzyme, at pH 6.0 and 30°C | Aspergillus terreus | |
23.5 | - |
3-Methoxybenzyl alcohol | recombinant enzyme, at pH 6.0 and 30°C | Aspergillus terreus | |
39.7 | - |
3,4-dimethoxybenzyl alcohol | recombinant enzyme, at pH 6.0 and 30°C | Aspergillus terreus | |
130.5 | - |
4-methoxybenzyl alcohol | at pH 6.0 and 30°C | Aspergillus terreus |