Inhibitors | Comment | Organism | Structure |
---|---|---|---|
Ag+ | 88% inhibition at 1 mM | Bordetella sp. | |
Hg2+ | 90% inhibition at 1 mM | Bordetella sp. | |
Na+ | slight inhibition at 1 mM | Bordetella sp. |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.556 | - |
cholesterol | pH 7.0, 37°C | Bordetella sp. |
Localization | Comment | Organism | GeneOntology No. | Textmining |
---|---|---|---|---|
extracellular | - |
Bordetella sp. | - |
- |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Ba2+ | activates slightly at 1 mM | Bordetella sp. | |
Ca2+ | 15% activation at 1 mM | Bordetella sp. | |
Co2+ | 10% activation at 1 mM | Bordetella sp. | |
Cu2+ | activates 2.19fold at 1 mM | Bordetella sp. | |
Fe3+ | activates slightly at 1 mM | Bordetella sp. | |
K+ | activates slightly at 1 mM | Bordetella sp. | |
Li+ | activates slightly at 1 mM | Bordetella sp. | |
Mg2+ | activates slightly at 1 mM | Bordetella sp. | |
Mn2+ | 25% activation at 1 mM | Bordetella sp. | |
Sr2+ | activates slightly at 1 mM | Bordetella sp. | |
Zn2+ | activates slightly at 1 mM | Bordetella sp. |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
55000 | - |
x * 55000, SDS-PAGE | Bordetella sp. |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
cholesterol + O2 | Bordetella sp. | - |
cholest-4-en-3-one + H2O2 | - |
? | |
cholesterol + O2 | Bordetella sp. B4 | - |
cholest-4-en-3-one + H2O2 | - |
? |
Organic Solvent | Comment | Organism |
---|---|---|
Acetone | 6% activity remaining, pH 7.0, 37°C, 24 h, with mechanical shaking | Bordetella sp. |
benzene | 127% activity remaining, pH 7.0, 37°C, 24 h, with mechanical shaking | Bordetella sp. |
Butanol | 77% activity remaining, pH 7.0, 37°C, 24 h, with mechanical shaking | Bordetella sp. |
chloroform | 96% activity remaining, pH 7.0, 37°C, 24 h, with mechanical shaking | Bordetella sp. |
cyclohexane | 119% activity remaining, pH 7.0, 37°C, 24 h, with mechanical shaking | Bordetella sp. |
dimethyl sulfoxide | 88% activity remaining, pH 7.0, 37°C, 24 h, with mechanical shaking | Bordetella sp. |
Ethanol | 5% activity remaining, pH 7.0, 37°C, 24 h, with mechanical shaking | Bordetella sp. |
Ethyl acetate | 93% activity remaining, pH 7.0, 37°C, 24 h, with mechanical shaking | Bordetella sp. |
Methanol | 4% activity remaining, pH 7.0, 37°C, 24 h, with mechanical shaking | Bordetella sp. |
xylene | 93% the activity remaining, pH 7.0, 37°C, 24 h, with mechanical shaking | Bordetella sp. |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Bordetella sp. | - |
isolated from a soil sample collected from the riverside of the Yellow River in Jinan City, Shandong Province, China | - |
Bordetella sp. B4 | - |
isolated from a soil sample collected from the riverside of the Yellow River in Jinan City, Shandong Province, China | - |
Purification (Comment) | Organism |
---|---|
native extracellular enzyme from medium 12fold to homogeneity by ammonium sulfate fractionation, dialysis, ultrafiltration, anion exchange chromatography and gel filtration | Bordetella sp. |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
20.81 | - |
purified enzyme, pH 7.0, 37°C | Bordetella sp. |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
cholesterol + O2 | - |
Bordetella sp. | cholest-4-en-3-one + H2O2 | - |
? | |
cholesterol + O2 | - |
Bordetella sp. B4 | cholest-4-en-3-one + H2O2 | - |
? |
Subunits | Comment | Organism |
---|---|---|
? | x * 55000, SDS-PAGE | Bordetella sp. |
Synonyms | Comment | Organism |
---|---|---|
COD | - |
Bordetella sp. |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
37 | - |
- |
Bordetella sp. |
Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|
30 | 40 | purified enzyme, stable at | Bordetella sp. |
50 | - |
purified enzyme, 90% or 80% activity remaining after 1 h or 2 h, respectively | Bordetella sp. |
60 | - |
purified enzyme, 20% activity remaining after 2 h | Bordetella sp. |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
7 | - |
- |
Bordetella sp. |
pH Minimum | pH Maximum | Comment | Organism |
---|---|---|---|
4 | 10 | activity range | Bordetella sp. |
pH Stability | pH Stability Maximum | Comment | Organism |
---|---|---|---|
4 | - |
purified enzyme, 4°C for 48 h, 80% activity remaining | Bordetella sp. |
5 | - |
purified enzyme, 4°C for 48 h, completely stable | Bordetella sp. |
6 | 10 | purified enzyme, 4°C for 48 h, 80% activity remaining | Bordetella sp. |