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Literature summary for 1.1.3.6 extracted from

  • Li, B.; Wang, W.; Wang, F.; Wei, D.
    Cholesterol oxidase ChoL is a critical enzyme that catalyzes the conversion of diosgenin to 4-ene-3-keto steroids in Streptomyces virginiae IBL-14 (2010), Appl. Microbiol. Biotechnol., 85, 1831-1838.
    View publication on PubMed

Cloned(Commentary)

Cloned (Comment) Organism
ChoL, a fragment of a cholesterol oxidase is expressed in Escherichia coli BL21(DE3) plysS cells Streptomyces virginiae

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.195
-
diosgenin pH 7.5, 37°C Streptomyces virginiae
0.491
-
dehydroepiandrosterone pH 7.5, 37°C Streptomyces virginiae
0.524
-
cholesterol pH 7.5, 37°C Streptomyces virginiae
0.59 1 sitosterol pH 7.5, 37°C Streptomyces virginiae

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
58900
-
calculated from amino acid sequence Streptomyces virginiae
59000
-
SDS-PAGE Streptomyces virginiae

Organism

Organism UniProt Comment Textmining
Streptomyces virginiae A7LGL0
-
-
Streptomyces virginiae IBL-14 A7LGL0
-
-

Purification (Commentary)

Purification (Comment) Organism
Ni2+-chelating Sepharose column chromatography Streptomyces virginiae

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
cholesterol + O2
-
Streptomyces virginiae cholest-4-en-3-one + H2O2
-
?
cholesterol + O2
-
Streptomyces virginiae IBL-14 cholest-4-en-3-one + H2O2
-
?
dehydroepiandrosterone + O2
-
Streptomyces virginiae androst-5-en-3,17-dione + H2O2
-
?
dehydroepiandrosterone + O2
-
Streptomyces virginiae IBL-14 androst-5-en-3,17-dione + H2O2
-
?
diosgenin + O2 i.e. (25R)-spirost-5-en-3beta-ol Streptomyces virginiae diosgenone + H2O2
-
?
diosgenin + O2 i.e. (25R)-spirost-5-en-3beta-ol Streptomyces virginiae IBL-14 diosgenone + H2O2
-
?
sitosterol + O2
-
Streptomyces virginiae ?
-
?
sitosterol + O2
-
Streptomyces virginiae IBL-14 ?
-
?

Synonyms

Synonyms Comment Organism
ChoL a fragment of a cholesterol oxidase Streptomyces virginiae

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
45
-
-
Streptomyces virginiae

Temperature Range [°C]

Temperature Minimum [°C] Temperature Maximum [°C] Comment Organism
35 48 ChoL retains more than 80% activity in the temperature range 35-48°C Streptomyces virginiae

Temperature Stability [°C]

Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
40 60 the enzyme is stable below 40°C without obvious inactivation after 6 h of incubation. When the temperature exceeds 50°C, the ChoL activity rapidly decreases. The enzyme retains approximately 40% of its initial activity after incubation for 6 h at 50°C and 17% after incubation for 1 h at 60°C Streptomyces virginiae

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
7.5
-
-
Streptomyces virginiae

pH Range

pH Minimum pH Maximum Comment Organism
6.5 9 ChoL retains more than 80% activity in the pH range 6.5-9.0 Streptomyces virginiae

Cofactor

Cofactor Comment Organism Structure
FAD
-
Streptomyces virginiae

pI Value

Organism Comment pI Value Maximum pI Value
Streptomyces virginiae calculated from amino acid sequence
-
8.5