Cloned (Comment) | Organism |
---|---|
ChoL, a fragment of a cholesterol oxidase is expressed in Escherichia coli BL21(DE3) plysS cells | Streptomyces virginiae |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.195 | - |
diosgenin | pH 7.5, 37°C | Streptomyces virginiae | |
0.491 | - |
dehydroepiandrosterone | pH 7.5, 37°C | Streptomyces virginiae | |
0.524 | - |
cholesterol | pH 7.5, 37°C | Streptomyces virginiae | |
0.59 | 1 | sitosterol | pH 7.5, 37°C | Streptomyces virginiae |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
58900 | - |
calculated from amino acid sequence | Streptomyces virginiae |
59000 | - |
SDS-PAGE | Streptomyces virginiae |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Streptomyces virginiae | A7LGL0 | - |
- |
Streptomyces virginiae IBL-14 | A7LGL0 | - |
- |
Purification (Comment) | Organism |
---|---|
Ni2+-chelating Sepharose column chromatography | Streptomyces virginiae |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
cholesterol + O2 | - |
Streptomyces virginiae | cholest-4-en-3-one + H2O2 | - |
? | |
cholesterol + O2 | - |
Streptomyces virginiae IBL-14 | cholest-4-en-3-one + H2O2 | - |
? | |
dehydroepiandrosterone + O2 | - |
Streptomyces virginiae | androst-5-en-3,17-dione + H2O2 | - |
? | |
dehydroepiandrosterone + O2 | - |
Streptomyces virginiae IBL-14 | androst-5-en-3,17-dione + H2O2 | - |
? | |
diosgenin + O2 | i.e. (25R)-spirost-5-en-3beta-ol | Streptomyces virginiae | diosgenone + H2O2 | - |
? | |
diosgenin + O2 | i.e. (25R)-spirost-5-en-3beta-ol | Streptomyces virginiae IBL-14 | diosgenone + H2O2 | - |
? | |
sitosterol + O2 | - |
Streptomyces virginiae | ? | - |
? | |
sitosterol + O2 | - |
Streptomyces virginiae IBL-14 | ? | - |
? |
Synonyms | Comment | Organism |
---|---|---|
ChoL | a fragment of a cholesterol oxidase | Streptomyces virginiae |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
45 | - |
- |
Streptomyces virginiae |
Temperature Minimum [°C] | Temperature Maximum [°C] | Comment | Organism |
---|---|---|---|
35 | 48 | ChoL retains more than 80% activity in the temperature range 35-48°C | Streptomyces virginiae |
Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|
40 | 60 | the enzyme is stable below 40°C without obvious inactivation after 6 h of incubation. When the temperature exceeds 50°C, the ChoL activity rapidly decreases. The enzyme retains approximately 40% of its initial activity after incubation for 6 h at 50°C and 17% after incubation for 1 h at 60°C | Streptomyces virginiae |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
7.5 | - |
- |
Streptomyces virginiae |
pH Minimum | pH Maximum | Comment | Organism |
---|---|---|---|
6.5 | 9 | ChoL retains more than 80% activity in the pH range 6.5-9.0 | Streptomyces virginiae |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
FAD | - |
Streptomyces virginiae |
Organism | Comment | pI Value Maximum | pI Value |
---|---|---|---|
Streptomyces virginiae | calculated from amino acid sequence | - |
8.5 |