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Literature summary for 1.1.3.6 extracted from

  • Toyama, M.; Yamashita, M.; Yoneda, M.; Zaborowski, A.; Nagato, M.; Ono, H.; Hirayama, N.; Murooka, Y.
    Alteration of substrate specificity of cholesterol oxidase from Streptomyces sp. by site-directed mutagenesis (2002), Protein Eng., 15, 477-484.
    View publication on PubMed
Search for more literature for 1.1.3.6

Cloned(Commentary)

Cloned (Comment) Organism
expression in Escherichia coli Streptomyces sp.

Protein Variants

Protein Variants Comment Organism
L117P mutant enzyme without activity Streptomyces sp.
L119A specific activity is similar to that of the wild-type enzyme Streptomyces sp.
L119F specific activity is similar to that of the wild-type enzyme Streptomyces sp.
P357N specific activity is similar to that of the wild-type enzyme Streptomyces sp.
Q286R specific activity is similar to that of the wild-type enzyme Streptomyces sp.
S279R mutant enzyme without activity on cholesterol and pregnenolone Streptomyces sp.
S379D mutant enzyme without activity on cholesterol and pregnenolone Streptomyces sp.
S379T specific activity is similar to that of the wild-type enzyme Streptomyces sp.
V145Q specific activity is similar to that of the wild-type enzyme Streptomyces sp.

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.0000058
-
 pregnenolone pH 7.0, 37°C, mutant enzyme S379T Streptomyces sp.
0.0000081
-
 cholesterol pH 7.0, 37°C, mutant enzyme S379T Streptomyces sp.
0.00002
-
 cholesterol pH 7.0, 37°C, wild-type enzyme Streptomyces sp.
0.000023
-
 pregnenolone pH 7.0, 37°C, wild-type enzyme Streptomyces sp.
0.000023
-
 pregnenolone pH 7.0, 37°C, mutant enzyme S379A Streptomyces sp.
0.00003
-
 cholesterol pH 7.0, 37°C, mutant enzyme S379A Streptomyces sp.
0.00004
-
 pregnenolone pH 7.0, 37°C, mutant enzyme L119A Streptomyces sp.
0.00004
-
 pregnenolone pH 7.0, 37°C, mutant enzyme S379V Streptomyces sp.
0.000046
-
 cholesterol pH 7.0, 37°C, mutant enzyme S379V Streptomyces sp.
0.000056
-
 pregnenolone pH 7.0, 37°C, mutant enzyme Q286R Streptomyces sp.
0.000058
-
 pregnenolone pH 7.0, 37°C, mutant enzyme V145Q Streptomyces sp.
0.000072
-
 cholesterol pH 7.0, 37°C, mutant enzyme V145Q Streptomyces sp.
0.000077
-
 pregnenolone pH 7.0, 37°C, mutant enzyme L119F Streptomyces sp.
0.000081
-
 cholesterol pH 7.0, 37°C, mutant enzyme L119A Streptomyces sp.
0.000083
-
 pregnenolone pH 7.0, 37°C, mutant enzyme V145E Streptomyces sp.
0.000098
-
 pregnenolone pH 7.0, 37°C, mutant enzyme P357N Streptomyces sp.
0.0001
-
 pregnenolone pH 7.0, 37°C, mutant enzyme V145D Streptomyces sp.
0.00011
-
 cholesterol pH 7.0, 37°C, mutant enzyme P357N Streptomyces sp.
0.00013
-
 cholesterol pH 7.0, 37°C, mutant enzyme Q286R Streptomyces sp.
0.00017
-
 cholesterol pH 7.0, 37°C, mutant enzyme L119F Streptomyces sp.
0.0004
-
 cholesterol pH 7.0, 37°C, mutant enzyme V145D Streptomyces sp.
0.00067
-
 cholesterol pH 7.0, 37°C, mutant enzyme V145E Streptomyces sp.

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
55000
-
 x * 55000, wild-type and mutant enzymes V145Q, V145E, S379A and S379V, SDS-PAGE Streptomyces sp.

Organism

Organism UniProt Comment Textmining
Streptomyces sp.
-
-
-

Purification (Commentary)

Purification (Comment) Organism
wild-type and mutant enzymes V145Q, V145E, S379A and S379V Streptomyces sp.

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
cholest-5-en-3beta-ol 7-one + O2 low activity with wild-type and mutant enzymes L119A, L119F, V145Q, P357N, Q286R and S379T Streptomyces sp. cholest-5-en-3,17-dione + H2O2
-
 ?
cholesterol + O2
-
 Streptomyces sp. cholest-4-en-3-one + H2O2
-
 ?
dehydroisoandrosterone + O2 low activity with wild-type and mutant enzymes L119A, L119F, V145Q, P357N, Q286R and S379T Streptomyces sp. androst-5-en-3,17-dione + H2O
-
 ?
dihydrocholesterol + O2
-
 Streptomyces sp. 5alpha-cholestan-3-one + H2O2
-
 ?
estrone + O2 low activity with wild-type enzyme, no activity with mutant enzymes L119A, L119F, V145Q, P357N, Q286R and S379T Streptomyces sp. estra-1,5(10)-dien-3,17-dione + H2O2
-
 ?
pregnenolone + O2
-
 Streptomyces sp. 5-pregnen-3,20-dione + H2O2
-
 ?

Subunits

Subunits Comment Organism
? x * 55000, wild-type and mutant enzymes V145Q, V145E, S379A and S379V, SDS-PAGE Streptomyces sp.

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
9
-
 cholesterol pH 7.0, 37°C, mutant enzyme P357N Streptomyces sp.
9
-
 pregnenolone pH 7.0, 37°C, mutant enzyme P357N Streptomyces sp.
9
-
 pregnenolone pH 7.0, 37°C, mutant enzyme V145D Streptomyces sp.
11
-
 cholesterol pH 7.0, 37°C, mutant enzyme V145D Streptomyces sp.
11
-
 pregnenolone pH 7.0, 37°C, mutant enzyme V145E Streptomyces sp.
11
-
 cholesterol pH 7.0, 37°C, mutant enzyme V145Q Streptomyces sp.
12
-
 pregnenolone pH 7.0, 37°C, mutant enzyme V145Q Streptomyces sp.
13
-
 pregnenolone pH 7.0, 37°C, mutant enzyme L119A Streptomyces sp.
15
-
 cholesterol pH 7.0, 37°C, mutant enzyme L119A Streptomyces sp.
19
-
 pregnenolone pH 7.0, 37°C, mutant enzyme Q286R Streptomyces sp.
21
-
 pregnenolone pH 7.0, 37°C, mutant enzyme L119F Streptomyces sp.
23
-
 cholesterol pH 7.0, 37°C, mutant enzyme Q286R Streptomyces sp.
23
-
 cholesterol pH 7.0, 37°C, mutant enzyme V145E Streptomyces sp.
27
-
 cholesterol pH 7.0, 37°C, mutant enzyme L119F Streptomyces sp.
29
-
 cholesterol pH 7.0, 37°C, mutant enzyme S379T Streptomyces sp.
30
-
 pregnenolone pH 7.0, 37°C, wild-type enzyme Streptomyces sp.
35
-
 pregnenolone pH 7.0, 37°C, mutant enzyme S379A Streptomyces sp.
39
-
 pregnenolone pH 7.0, 37°C, mutant enzyme S379V Streptomyces sp.
40
-
 cholesterol pH 7.0, 37°C, wild-type enzyme Streptomyces sp.
45
-
 pregnenolone pH 7.0, 37°C, mutant enzyme S379T Streptomyces sp.
49
-
 cholesterol pH 7.0, 37°C, mutant enzyme S379A Streptomyces sp.
51
-
 cholesterol pH 7.0, 37°C, mutant enzyme S379V Streptomyces sp.