KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
4.5 | - |
cholesterol | pH 7, 30°C, enzyme CO2 | Bacillus sp. (in: Bacteria) | |
6.76 | - |
cholesterol | pH 7, 30°C, enzyme CO1 | Bacillus sp. (in: Bacteria) |
Localization | Comment | Organism | GeneOntology No. | Textmining |
---|---|---|---|---|
extracellular | - |
Bacillus sp. (in: Bacteria) | - |
- |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
36000 | - |
1 * 36000, enzyme CO1, SDS-PAGE | Bacillus sp. (in: Bacteria) |
37000 | - |
1 * 37000, enzyme CO2, SDS-PAGE | Bacillus sp. (in: Bacteria) |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Bacillus sp. (in: Bacteria) | - |
SFF34 | - |
Bacillus sp. (in: Bacteria) SFF34 | - |
SFF34 | - |
Purification (Comment) | Organism |
---|---|
- |
Bacillus sp. (in: Bacteria) |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
7.6 | - |
enzyme CO1 | Bacillus sp. (in: Bacteria) |
8 | - |
enzyme CO2 | Bacillus sp. (in: Bacteria) |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
1,4-androstadiene-3,17-dione + H2O | 11% of the activity with cholesterol, enzyme CO1 | Bacillus sp. (in: Bacteria) | ? | - |
? | |
5alpha-cholestane + O2 | 72% of the activity with cholesterol, enzyme CO1 | Bacillus sp. (in: Bacteria) | ? | - |
? | |
5alpha-cholestane + O2 | 90% of the activity with cholesterol, enzyme CO2 | Bacillus sp. (in: Bacteria) | ? | - |
? | |
5alpha-cholestane-3beta-ol-7-one + O2 | 74% of the activity with cholesterol, enzyme CO1 | Bacillus sp. (in: Bacteria) | 5alpha-cholestane-3,7,dione | - |
? | |
5alpha-cholestane-3beta-ol-7-one + O2 | 84% of the activity with cholesterol, enzyme CO2 | Bacillus sp. (in: Bacteria) | 5alpha-cholestane-3,7,dione | - |
? | |
beta-sitosterol + O2 | 57% of the activity with cholesterol, enzyme CO1 | Bacillus sp. (in: Bacteria) | stigmast-5-en-3-one + H2O2 | - |
? | |
beta-sitosterol + O2 | 79% of the activity with cholesterol, enzyme CO2 | Bacillus sp. (in: Bacteria) | stigmast-5-en-3-one + H2O2 | - |
? | |
beta-sitosterol + O2 | 57% of the activity with cholesterol, enzyme CO1 | Bacillus sp. (in: Bacteria) SFF34 | stigmast-5-en-3-one + H2O2 | - |
? | |
beta-sitosterol + O2 | 79% of the activity with cholesterol, enzyme CO2 | Bacillus sp. (in: Bacteria) SFF34 | stigmast-5-en-3-one + H2O2 | - |
? | |
cholesterol + O2 | - |
Bacillus sp. (in: Bacteria) | cholest-4-en-3-one + H2O2 | - |
? | |
cholesterol + O2 | - |
Bacillus sp. (in: Bacteria) SFF34 | cholest-4-en-3-one + H2O2 | - |
? | |
cholesterol-5alpha,6beta-epoxide + O2 | 10% of the activity with cholesterol, enzyme CO2 | Bacillus sp. (in: Bacteria) | ? | - |
? | |
cholesterol-5alpha,6beta-epoxide + O2 | 9% of the activity with cholesterol, enzyme CO1 | Bacillus sp. (in: Bacteria) | ? | - |
? | |
coprostane + O2 | 63% of the activity with cholesterol, enzyme CO1 | Bacillus sp. (in: Bacteria) | ? | - |
? | |
coprostane + O2 | 67% of the activity with cholesterol, enzyme CO2 | Bacillus sp. (in: Bacteria) | ? | - |
? | |
dihydrocholesterol + O2 | 58% of the activity with cholesterol, enzyme CO1 | Bacillus sp. (in: Bacteria) | 5alpha-cholestan-3-one + H2O2 | - |
? | |
dihydrocholesterol + O2 | 70% of the activity with cholesterol, enzyme CO2 | Bacillus sp. (in: Bacteria) | 5alpha-cholestan-3-one + H2O2 | - |
? | |
hecogenin + O2 | 66% of the activity with cholesterol, enzyme CO1 | Bacillus sp. (in: Bacteria) | 5alpha-(25R)-spirostan-3,12-dione + H2O2 | - |
? | |
hecogenin + O2 | 80% of the activity with cholesterol, enzyme CO2 | Bacillus sp. (in: Bacteria) | 5alpha-(25R)-spirostan-3,12-dione + H2O2 | - |
? | |
lanosterol + O2 | 4% of the activity with cholesterol, enzyme CO1 | Bacillus sp. (in: Bacteria) | 5alpha-lanosta-8,24-dien-3-one + H2O2 | - |
? | |
lanosterol + O2 | 5% of the activity with cholesterol, enzyme CO2 | Bacillus sp. (in: Bacteria) | 5alpha-lanosta-8,24-dien-3-one + H2O2 | - |
? | |
stigmasterol + O2 | 102% of the activity with cholesterol, enzyme CO2 | Bacillus sp. (in: Bacteria) | stigmasta-5,22-dien-3-one + H2O2 | - |
? | |
stigmasterol + O2 | 86% of the activity with cholesterol, enzyme CO1 | Bacillus sp. (in: Bacteria) | stigmasta-5,22-dien-3-one + H2O2 | - |
? | |
stigmasterol + O2 | 102% of the activity with cholesterol, enzyme CO2 | Bacillus sp. (in: Bacteria) SFF34 | stigmasta-5,22-dien-3-one + H2O2 | - |
? | |
stigmasterol + O2 | 86% of the activity with cholesterol, enzyme CO1 | Bacillus sp. (in: Bacteria) SFF34 | stigmasta-5,22-dien-3-one + H2O2 | - |
? |
Subunits | Comment | Organism |
---|---|---|
monomer | 1 * 36000, enzyme CO1, SDS-PAGE | Bacillus sp. (in: Bacteria) |
monomer | 1 * 37000, enzyme CO2, SDS-PAGE | Bacillus sp. (in: Bacteria) |
Synonyms | Comment | Organism |
---|---|---|
CO1 | - |
Bacillus sp. (in: Bacteria) |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
40 | - |
reaction with cholesterol, enzyme CO2 | Bacillus sp. (in: Bacteria) |
60 | - |
reaction with cholesterol, enzyme CO1 | Bacillus sp. (in: Bacteria) |
Temperature Minimum [°C] | Temperature Maximum [°C] | Comment | Organism |
---|---|---|---|
30 | 55 | 30°C: about 65% of maximal activity, 55°C: about 60% of maximal activity, reaction with cholesterol, enzyme CO2 | Bacillus sp. (in: Bacteria) |
35 | 75 | about 50% of maximal activity at 35°C and at 75°C, reaction with cholesterol, enzyme CO1 | Bacillus sp. (in: Bacteria) |
Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|
60 | - |
1 h, about 10% loss of activity, enzyme CO1 | Bacillus sp. (in: Bacteria) |
60 | - |
1 h, about 10% loss of activity, enzyme CO2 | Bacillus sp. (in: Bacteria) |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
6 | - |
reaction with cholesterol, enzyme CO2 | Bacillus sp. (in: Bacteria) |
6.5 | - |
reaction with cholesterol, enzyme CO1 | Bacillus sp. (in: Bacteria) |
pH Minimum | pH Maximum | Comment | Organism |
---|---|---|---|
5 | 9 | pH 5: 83% of maximal activity, pH 9.0: 77% of maximal activity, reaction with cholesterol, enzyme CO1 | Bacillus sp. (in: Bacteria) |
5 | 8 | pH 5: 78% of maximal activity, pH 8.0: 75% of maximal activity, reaction with cholestero, enzyme CO2 | Bacillus sp. (in: Bacteria) |
pH Stability | pH Stability Maximum | Comment | Organism |
---|---|---|---|
5 | - |
most stable at, enzyme CO2 | Bacillus sp. (in: Bacteria) |
6 | - |
most stable at | Bacillus sp. (in: Bacteria) |