Protein Variants | Comment | Organism |
---|---|---|
E361Q | turnover number for cholesterol is decreased 31.4fold compared to wild-type enzyme, 1.8fold increase in Km-value for cholesterol compared to wild-type enzyme | Streptomyces sp. |
H447E | turnover number for cholesterol is decreased about 2600fold compared to wild-type enzyme | Streptomyces sp. |
H447E/E361Q | turnover number for cholesterol is decreased about 31500fold compared to wild-type enzyme. Mutant enzyme is not able to isomerize cholest-5-en-3-one. Mutant enzyme is folded like native enzyme and still associates with model membranes | Streptomyces sp. |
H447Q | turnover number for cholesterol is decreased 137.5fold compared to wild-type enzyme, Km-value for cholesterol is identical to wild-type value | Streptomyces sp. |
H447Q/E361Q | turnover number for cholesterol is decreased 473fold compared to wild-type enzyme, 1.7fold increase in Km-value for cholesterol compared to wild-type enzyme. Mutant enzyme is not able to isomerize cholest-5-en-3-one | Streptomyces sp. |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.003 | - |
cholesterol | wild-type enzyme | Streptomyces sp. | |
0.003 | - |
cholesterol | mutant enzyme H447Q | Streptomyces sp. | |
0.005 | - |
cholesterol | mutant enzyme H447Q/E361Q | Streptomyces sp. | |
0.0054 | - |
cholesterol | mutant enzyme E361 | Streptomyces sp. |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Streptomyces sp. | - |
- |
- |
Purification (Comment) | Organism |
---|---|
wild-type and mutant enzymes | Streptomyces sp. |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
cholesterol + O2 | - |
Streptomyces sp. | cholest-5-en-3-one + H2O2 | subsequent isomerization to cholest-4-en-3-one | ? |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.0014 | - |
cholesterol | mutant enzyme H447E/E361Q | Streptomyces sp. | |
0.017 | - |
cholesterol | mutant enzyme H447E | Streptomyces sp. | |
0.093 | - |
cholesterol | mutant enzyme H447Q/E361Q | Streptomyces sp. | |
0.32 | - |
cholesterol | mutant enzyme H447Q | Streptomyces sp. | |
1.4 | - |
cholesterol | mutant enzyme E361 | Streptomyces sp. | |
44 | - |
cholesterol | wild-type enzyme | Streptomyces sp. |