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Literature summary for 1.1.3.46 extracted from

  • Liu, S.P.; Liu, R.X.; El-Rotail, A.A.; Ding, Z.Y.; Gu, Z.H.; Zhang, L.; Shi, G.Y.
    Heterologous pathway for the production of L-phenylglycine from glucose by E. coli (2014), J. Biotechnol., 186C, 91-97.
    View publication on PubMed

Cloned(Commentary)

Cloned (Comment) Organism
expressed in Escherichia coli BL21(DE3) cells Streptomyces coelicolor
expressed in Escherichia coli BL21(DE3) cells Amycolatopsis orientalis

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
(S)-4-hydroxymandelate + O2 Streptomyces coelicolor
-
2-(4-hydroxyphenyl)-2-oxoacetate + H2O2
-
?
(S)-4-hydroxymandelate + O2 Amycolatopsis orientalis
-
2-(4-hydroxyphenyl)-2-oxoacetate + H2O2
-
?
(S)-4-hydroxymandelate + O2 Streptomyces coelicolor CGMCC 4.1092
-
2-(4-hydroxyphenyl)-2-oxoacetate + H2O2
-
?
(S)-4-hydroxymandelate + O2 Amycolatopsis orientalis ATCC 19795
-
2-(4-hydroxyphenyl)-2-oxoacetate + H2O2
-
?

Organism

Organism UniProt Comment Textmining
Amycolatopsis orientalis
-
-
-
Amycolatopsis orientalis ATCC 19795
-
-
-
Streptomyces coelicolor
-
-
-
Streptomyces coelicolor CGMCC 4.1092
-
-
-

Purification (Commentary)

Purification (Comment) Organism
HisTrap affinity column chromatography Streptomyces coelicolor
HisTrap affinity column chromatography Amycolatopsis orientalis

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
310
-
pH and temperature not specified in the publication Amycolatopsis orientalis
1822
-
pH and temperature not specified in the publication Streptomyces coelicolor

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
(S)-4-hydroxymandelate + O2
-
Streptomyces coelicolor 2-(4-hydroxyphenyl)-2-oxoacetate + H2O2
-
?
(S)-4-hydroxymandelate + O2
-
Amycolatopsis orientalis 2-(4-hydroxyphenyl)-2-oxoacetate + H2O2
-
?
(S)-4-hydroxymandelate + O2
-
Streptomyces coelicolor CGMCC 4.1092 2-(4-hydroxyphenyl)-2-oxoacetate + H2O2
-
?
(S)-4-hydroxymandelate + O2
-
Amycolatopsis orientalis ATCC 19795 2-(4-hydroxyphenyl)-2-oxoacetate + H2O2
-
?

Synonyms

Synonyms Comment Organism
HmO
-
Streptomyces coelicolor
HmO
-
Amycolatopsis orientalis
L-4-hydroxymandelate oxidase
-
Streptomyces coelicolor
L-4-hydroxymandelate oxidase
-
Amycolatopsis orientalis

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
50
-
-
Streptomyces coelicolor
60
-
-
Amycolatopsis orientalis