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Literature summary for 1.1.3.4 extracted from

  • Zargari, A.; Selander, C.; Rasool, O.; Ghanem, M.; Gadda, G.; Crameri, R.; Scheynius, A.
    Mala s12 is a major allergen in patients with atopic eczema and has sequence similarities to the GMC oxidoreductase family (2007), Allergy, 62, 695-703.
    View publication on PubMed
Search for more literature for 1.1.3.4

Application

Application Comment Organism
medicine analysis of the chronic inflammatory skin disorder Atopic eczema (AE) Malassezia sympodialis

Cloned(Commentary)

Cloned (Comment) Organism
recombinant protein, expressed in Escherichia coli strain BL21, pET17b expression vector Malassezia sympodialis

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
additional information
-
 additional information choline, glucose, myo-inositol, methanol, ethanol, 1-pentanol, benzyl alcohol, 2-phenylethanol, cholesterol or lauryl alcohol tested as potential substrates, recombinant protein devoid of either oxidase or dehydrogenase activity Malassezia sympodialis

Organism

Organism UniProt Comment Textmining
Malassezia sympodialis
-
 strain ATCC 42132
-

Purification (Commentary)

Purification (Comment) Organism
recombinant protein, gel filtration Malassezia sympodialis

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
additional information
-
 sequence similarity to the glucose-methanol-choline (GMC) oxidoreductase enzyme superfamily, biophysical properties shown, function as an electron transport protein, no enzyme activity of the recombinant protein, major allergen in Atopic eczema patients Malassezia sympodialis

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
additional information characterization of the allergen Mala s12, sequence similarity to glucose-methanol-choline (GMC) oxidoreductase enzyme superfamily, no enzyme activity of the recombinant protein in oxidase or dehydrogenase assay determined Malassezia sympodialis ?
-
 ?

Cofactor

Cofactor Comment Organism Structure
FAD covalently bound to the recombinant protein of Mala s12, flavin content per monomer of protein calculated Malassezia sympodialis