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Literature summary for 1.1.3.4 extracted from

  • Crognale, S.; Pulci, V.; Brozzoli, V.; Petruccioli, M.; Federici, F.
    Expression of Penicillium variabile P16 glucose oxidase gene in Pichia pastoris and characterization of the recombinant enzyme (2006), Enzyme Microb. Technol., 39, 1230-1235.
No PubMed abstract available
Search for more literature for 1.1.3.4

Application

Application Comment Organism
analysis the enzyme finds wide application in food industry and clinical analysis Talaromyces variabilis

Cloned(Commentary)

Cloned (Comment) Organism
gene GOX, DNA sequence determination and analysis, expression in a larger scale in Pichia pastoris strain X33 using the methanol inducible AOX1 promoter, the enzyme is secreted Talaromyces variabilis

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
13.5
-
 D-glucose pH 5.0, 28°C, native enzyme Talaromyces variabilis
15.25
-
 D-glucose pH 5.0, 28°C, recombinant enzyme Talaromyces variabilis

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
82000
-
 2 * 82000, recombinant enzyme, SDS-PAGE Talaromyces variabilis
175000
-
 gel filtration Talaromyces variabilis

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
D-glucose + O2 Talaromyces variabilis
-
 D-glucono-1,5-lactone + H2O2
-
 ?
D-glucose + O2 Talaromyces variabilis P16
-
 D-glucono-1,5-lactone + H2O2
-
 ?

Organism

Organism UniProt Comment Textmining
Talaromyces variabilis Q70FC9
-
-
Talaromyces variabilis P16 Q70FC9
-
-

Posttranslational Modification

Posttranslational Modification Comment Organism
glycoprotein the recombinant enzyme from Pichia pastoris is higher glycosylated, 17%, compared to the native enzyme, 14% Talaromyces variabilis

Purification (Commentary)

Purification (Comment) Organism
recombinant enzyme 2.4fold from Pichia pastoris culture supernatant by ultrafiltration, anion exchange chromatography, and gel filtration Talaromyces variabilis

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
0.33
-
 secreted enzyme in crude cell culture supernatant Talaromyces variabilis
144
-
 purified recombinant enzyme Talaromyces variabilis

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2-deoxy-D-glucose + O2 19.6% of the activity with D-glucose for the native enzyme, 5.9 for the recombinant enzyme Talaromyces variabilis ?
-
 ?
D-fructose + O2 4.9% of the activity with D-glucose for the native enzyme, no activity with the recombinant enzyme Talaromyces variabilis ?
-
 ?
D-glucose + O2
-
 Talaromyces variabilis D-glucono-1,5-lactone + H2O2
-
 ?
D-glucose + O2 best substrate Talaromyces variabilis D-glucono-1,5-lactone + H2O2
-
 ?
D-glucose + O2
-
 Talaromyces variabilis P16 D-glucono-1,5-lactone + H2O2
-
 ?
D-glucose + O2 best substrate Talaromyces variabilis P16 D-glucono-1,5-lactone + H2O2
-
 ?
D-mannose + O2 7.2% of the activity with D-glucose for the native enzyme, 13.4 for the recombinant enzyme Talaromyces variabilis ?
-
 ?
D-mannose + O2 7.2% of the activity with D-glucose for the native enzyme, 13.4 for the recombinant enzyme Talaromyces variabilis P16 ?
-
 ?
D-xylose + O2 3.0% of the activity with D-glucose for the native enzyme, 5.8 for the recombinant enzyme Talaromyces variabilis ?
-
 ?
D-xylose + O2 3.0% of the activity with D-glucose for the native enzyme, 5.8 for the recombinant enzyme Talaromyces variabilis P16 ?
-
 ?
maltose + O2 21.3% of the activity with D-glucose for the native enzyme, 42.2% for the recombinant enzyme Talaromyces variabilis ?
-
 ?
additional information no activity with L-arabinose and D-galactose with the native and recombinant enzyme Talaromyces variabilis ?
-
 ?
additional information no activity with L-arabinose and D-galactose with the native and recombinant enzyme Talaromyces variabilis P16 ?
-
 ?

Subunits

Subunits Comment Organism
dimer 2 * 82000, recombinant enzyme, SDS-PAGE Talaromyces variabilis

Synonyms

Synonyms Comment Organism
GOX
-
 Talaromyces variabilis

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
45
-
 recombinant enzyme Talaromyces variabilis
55
-
 native enzyme Talaromyces variabilis

Temperature Range [°C]

Temperature Minimum [°C] Temperature Maximum [°C] Comment Organism
30 60 temperature profile of native enzyme, overview Talaromyces variabilis
30 55 temperature profile of recombinant enzyme, overview Talaromyces variabilis

Temperature Stability [°C]

Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
40
-
 half-life native enzyme: 103 min, half-life recombinant enzyme: 26 min Talaromyces variabilis
45
-
 half-life native enzyme: 23 min, half-life recombinant enzyme: 3 min Talaromyces variabilis

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
additional information
-
 additional information native enzyme and recombinant enzyme at pH 5.0, 28°C Talaromyces variabilis

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
6
-
 native and recombinant enzyme Talaromyces variabilis

pH Range

pH Minimum pH Maximum Comment Organism
3 8 pH-profiles, 54% of maximal activity at pH 3.0, 20% of maximal activity at pH 8.0 for the recombinant enzyme, 50% for the native enzyme, overview Talaromyces variabilis

pH Stability

pH Stability pH Stability Maximum Comment Organism
3
-
 half-life native enzyme: 166.3 min Talaromyces variabilis
7
-
 half-life native enzyme: 76.3 min Talaromyces variabilis

Cofactor

Cofactor Comment Organism Structure
FAD bound Talaromyces variabilis