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Literature summary for 1.1.3.4 extracted from

  • Ko, J.H.; Hahm, M.S.; Kang, H.A.; Nam, S.W.; Chung, B.H.
    Secretory expression and purification of Aspergillus niger glucose oxidase in Saccharomyces cerevisiae mutant deficient in PMR1 gene (2002), Protein Expr. Purif., 25, 488-493.
    View publication on PubMed

Cloned(Commentary)

Cloned (Comment) Organism
expression in Saccharomyces cerevisiae mutant deficient in PMR1 gene. GOD-His6 expressed in wild-type strain is hyperglycosylated, GOD-His6 expressed in pmr1DELTA strain is not hyperglycosylated Aspergillus niger

Organism

Organism UniProt Comment Textmining
Aspergillus niger
-
-
-

Posttranslational Modification

Posttranslational Modification Comment Organism
glycoprotein GOD-His6 expressed in wild-type strain of Saccharomyces cerevisiae is hyperglycosylated, GOD-His6 expressed in pmr1DELTA strain of Saccharomyces cerevisiae is not hyperglycosylated. The catalytic efficiency and physical properties of pmr1DELTA mutant-derived GOD-His6 are very similar to those of natural GOD Aspergillus niger

Purification (Commentary)

Purification (Comment) Organism
-
Aspergillus niger

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
189
-
GOD-His6 expressed in wild-type cells of Saccharomyces cerevisiae Aspergillus niger
194
-
GOD-His6 expressed in pmr1DELTA mutant cells of Saccharomyces cerevisiae Aspergillus niger

Temperature Range [°C]

Temperature Minimum [°C] Temperature Maximum [°C] Comment Organism
70
-
pH 5.1, 75% loss of of activity of natural GOD and pmr1DELTA-mutant-derived GOD, 65% loss of activity of hyperglycosylated GOD Aspergillus niger

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
6
-
natural GOD, pmr1DELTA-mutant-derived GOD,hyperglycosylated GOD expressed in wild-type cells of Saccharomyces cerevisiae Aspergillus niger

pH Range

pH Minimum pH Maximum Comment Organism
4.5 10 pH 4.5: about 85% of maximal activity, natural GOD and pmr1DELTA-mutant-derived GOD, about 90% of maximal activity, hyperglycosylated GOD expressed in wild-type cells of Saccharomyces cerevisiae, pH 10: about 75% of maximal activity, natural GOD and pmr1DELTA-mutant-derived GOD, about 90% of maximal activity, hyperglycosylated GOD expressed in wild-type cells of Saccharomyces cerevisiae Aspergillus niger