Cloned (Comment) | Organism |
---|---|
isolation of the gene encoding the enzyme, DNA sequence analysis of the coding region shows 80% identity to the sequence of a enzyme gene previously published | Aspergillus niger |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
23.7 | - |
beta-D-glucose | BTL wild-type strain enzyme | Aspergillus niger | |
31.8 | - |
beta-D-glucose | commercially available enzyme | Aspergillus niger |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
75000 | - |
x * 75000, SDS-PAGE | Aspergillus niger |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Aspergillus niger | - |
wild-type strain denoted BTL | - |
Purification (Comment) | Organism |
---|---|
from BTL wild-type strain using ammonium sulfate precipitation, column chromatography on Q-Sepharose at pH 6 and pH 4.5 and gel filtration on SW-300 | Aspergillus niger |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
additional information | - |
- |
Aspergillus niger |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
beta-D-glucose + O2 + H2O | - |
Aspergillus niger | D-glucono-1,5-lactone + H2O2 | - |
? |
Subunits | Comment | Organism |
---|---|---|
? | x * 75000, SDS-PAGE | Aspergillus niger |
Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|
40 | - |
at or below both the BTL wild-type strain enzyme and the commercially available enzyme are stable for at least 48 h | Aspergillus niger |
55 | - |
the commercially available enzyme is more stable than the BTL wild-type strain enzyme | Aspergillus niger |
65 | - |
the commercially available enzyme is more stable than the BTL wild-type strain enzyme | Aspergillus niger |
pH Stability | pH Stability Maximum | Comment | Organism |
---|---|---|---|
2.5 | - |
the BTL wild-type strain enzyme is slightly more stable than the commercially available enzyme | Aspergillus niger |
3 | - |
the BTL wild-type strain enzyme is slightly more stable than the commercially available enzyme | Aspergillus niger |
4 | - |
both the BTL wild-type strain enzyme and the commercially available enzyme are stable for at least 48 h | Aspergillus niger |
5 | - |
both the BTL wild-type strain enzyme and the commercially available enzyme are stable for at least 48 h | Aspergillus niger |
6 | - |
both the BTL wild-type strain enzyme and the commercially available enzyme are stable for at least 48 h | Aspergillus niger |