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Literature summary for 1.1.3.4 extracted from

  • Rogalski, J.; Fiedurek, J.; Szczordrak, J.; Kapusta, K.; Leonowicz, A.
    Optimization of glucose oxidase synthesis in submerged cultures of Aspergillus niger G-13 mutant (1988), Enzyme Microb. Technol., 10, 508-511.
No PubMed abstract available
Search for more literature for 1.1.3.4

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
beta-D-glucose + O2 + H2O Aspergillus niger
-
 D-glucono-1,5-lactone + H2O2
-
 ?

Organism

Organism UniProt Comment Textmining
Aspergillus niger
-
 G-13 mutant, culture conditions
-

Purification (Commentary)

Purification (Comment) Organism
partial Aspergillus niger

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
3.1
-
 in the culture filtrate Aspergillus niger
6
-
 after isolation from the fermenter liquid by gel filtration, lyophilized preparate Aspergillus niger

Storage Stability

Storage Stability Organism
4°C, lyophilized enzyme, no loss of activity after 1 year, 10% loss of activity after 2 years Aspergillus niger

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2-deoxy-D-glucose + O2 + H2O 30% relative activity to beta-D-glucose Aspergillus niger 2-deoxy-D-glucono-1,5-lactone + H2O2
-
 ?
beta-D-glucose + O2 + H2O
-
 Aspergillus niger D-glucono-1,5-lactone + H2O2
-
 ?

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
45
-
-
 Aspergillus niger

Temperature Stability [°C]

Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
40
-
 60 min Aspergillus niger

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
5.6
-
-
 Aspergillus niger

pH Stability

pH Stability pH Stability Maximum Comment Organism
4
-
 rapid inactivation below Aspergillus niger
6 8
-
 Aspergillus niger