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Literature summary for 1.1.3.37 extracted from

  • Kim, S.T.; Huh, W.K.; Kim, J.Y.; Hwang, S.W.; Kang, S.O.
    D-Arabinose dehydrogenase and biosynthesis of erythroascorbic acid in Candida albicans (1996), Biochim. Biophys. Acta, 1297, 1-8.
    View publication on PubMed
Search for more literature for 1.1.3.37

General Stability

General Stability Organism
repeated freezing at -20°C and thawing results in rapid and irreversible loss of activity Candida albicans

Inhibitors

Inhibitors Comment Organism Structure
Cd2+
-
 Candida albicans
D-galactono-1,4-lactone competitive to D-arabinono-1,4-lactone Candida albicans
D-glucono-1,5-lactone competitive to D-arabinono-1,4-lactone Candida albicans
D-gulono-1,4-lactone competitive to D-arabinono-1,4-lactone Candida albicans
Hg2+
-
 Candida albicans
iodoacetamide
-
 Candida albicans
iodoacetic acid
-
 Candida albicans
L-arabinono-1,4-lactone competitive to D-arabinono-1,4-lactone Candida albicans
Mn2+
-
 Candida albicans
NEM
-
 Candida albicans
PCMB
-
 Candida albicans
Zn2+
-
 Candida albicans

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
44.1
-
 D-arabinono-1,4-lactone
-
 Candida albicans
53.7
-
 L-galactono-1,4-lactone
-
 Candida albicans

Localization

Localization Comment Organism GeneOntology No. Textmining
mitochondrion
-
 Candida albicans 5739
-

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
66700
-
 1 * 66700, SDS-PAGE Candida albicans
84400
-
 PAGE in presence of deoxycholate Candida albicans
110000
-
 gel filtration Candida albicans

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
D-arabinono-1,4-lactone + O2 Candida albicans final step in biosynthesis of D-erythroascorbic acid ?
-
 ?

Organism

Organism UniProt Comment Textmining
Candida albicans
-
 ATCC 10231
-

Purification (Commentary)

Purification (Comment) Organism
-
 Candida albicans

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
18.8
-
-
 Candida albicans

Storage Stability

Storage Stability Organism
4°C, less than 50% loss of activity after 2 months Candida albicans

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
D-Arabinono-1,4-lactone + O2
-
 Candida albicans D-erythro-ascorbate + ?
-
 ?
D-arabinono-1,4-lactone + O2 final step in biosynthesis of D-erythroascorbic acid Candida albicans ?
-
 ?
L-Galactono-1,4-lactone + O2 87.6% of the activity relative to D-arabinono-1,4-lactone Candida albicans ?
-
 ?
L-Gulono-1,4-lactone + O2 24.7% of the activity relative to D-arabinono-1,4-lactone Candida albicans ?
-
 ?
L-Xylono-1,4-lactone + O2 84.6% of the activity relative to D-arabinono-1,4-lactone Candida albicans ?
-
 ?

Subunits

Subunits Comment Organism
monomer 1 * 66700, SDS-PAGE Candida albicans

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
40
-
-
 Candida albicans

Temperature Stability [°C]

Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
50
-
 above, 1 min, complete loss of activity Candida albicans

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
18.3
-
 L-galactono-1,4-lactone
-
 Candida albicans
20.9
-
 D-arabinono-1,4-lactone
-
 Candida albicans

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
6.1
-
-
 Candida albicans

pH Range

pH Minimum pH Maximum Comment Organism
5 8 pH 5.0: about 20% of maximal activity, pH 8.0: about 40% of maximal activity Candida albicans

pH Stability

pH Stability pH Stability Maximum Comment Organism
7.5 10 4°C, 48 h, stable Candida albicans

Cofactor

Cofactor Comment Organism Structure
flavin flavoprotein, the flavin may be covalently bound to the enzyme Candida albicans