Any feedback?
Please rate this page
(literature.php)
(0/150)

BRENDA support

Literature summary for 1.1.3.30 extracted from

  • Shimao, M.; Ninomiyaaa, K.; Kuno, O.; Kato, N.; Sakazawa, C.
    Existence of a novel enzyme, pyrroloquinoline quinone-dependent polyvinyl alcohol dehydrogenase, in a bacterial symbiont, Pseudomonas sp. strain VM15C (1986), Appl. Environ. Microbiol., 51, 268-275.
    View publication on PubMedView publication on EuropePMC
Search for more literature for 1.1.3.30

Localization

Localization Comment Organism GeneOntology No. Textmining
extracellular amount depending on growth rate Pseudomonas sp.
-
-
periplasm
-
 Pseudomonas sp.
-
-

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
31000
-
 gel filtration Pseudomonas sp.

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
polyvinyl alcohol + O2 Pseudomonas sp.
-
 oxidized polyvinyl alcohol + H2O2
-
 ?
polyvinyl alcohol + O2 Pseudomonas sp. VM15C
-
 oxidized polyvinyl alcohol + H2O2
-
 ?

Organism

Organism UniProt Comment Textmining
Pseudomonas sp.
-
 in symbiotic culture with Pseudomonas putida during PVA utilization
-

Purification (Commentary)

Purification (Comment) Organism
-
 Pseudomonas sp.

Source Tissue

Source Tissue Comment Organism Textmining
culture supernatant
-
 Pseudomonas sp.
-

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
1.68
-
 polyvinyl alcohol Pseudomonas sp.

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2,5-hexanediol + O2 4.9% of activity with polyvinyl alcohol Pseudomonas sp. 2,5-hexanedione + H2O2
-
 ?
2-hexanol + O2 101% of activity with polyvinyl alcohol Pseudomonas sp. 2-hexanone + H2O2
-
 ?
2-hexanol + O2 101% of activity with polyvinyl alcohol Pseudomonas sp. VM15C 2-hexanone + H2O2
-
 ?
2-pentanol + O2 58% of activity with polyvinyl alcohol Pseudomonas sp. 2-pentanone + H2O2
-
 ?
2-pentanol + O2 58% of activity with polyvinyl alcohol Pseudomonas sp. VM15C 2-pentanone + H2O2
-
 ?
2-propanol + O2 4.9% of activity with polyvinyl alcohol Pseudomonas sp. acetone + H2O2
-
 ?
2-propanol + O2 4.9% of activity with polyvinyl alcohol Pseudomonas sp. VM15C acetone + H2O2
-
 ?
4-heptanol + O2 91% of activity with polyvinyl alcohol Pseudomonas sp. 4-heptanone + H2O2
-
 ?
4-heptanol + O2 91% of activity with polyvinyl alcohol Pseudomonas sp. VM15C 4-heptanone + H2O2
-
 ?
polyvinyl alcohol + O2
-
 Pseudomonas sp. oxidized polyvinyl alcohol + H2O2
-
 ?
polyvinyl alcohol + O2
-
 Pseudomonas sp. VM15C oxidized polyvinyl alcohol + H2O2
-
 ?

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
30
-
 assay at Pseudomonas sp.

Cofactor

Cofactor Comment Organism Structure
additional information no requirement for pyrroloquinoline quinone (PQQ) as does the PQQ-dependent PVA dehydrogenase Pseudomonas sp.