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Literature summary for 1.1.3.17 extracted from

  • Enokibara, S.
    Purification and characterization of an alkaliphilic choline oxidase of Fusarium oxysporum (2012), Biosci. Biotechnol. Biochem., 76, 2219-2224.
    View publication on PubMed

Inhibitors

Inhibitors Comment Organism Structure
4-chloromercuribenzoate 75% inhibition at 1 mM Fusarium oxysporum
Cu2+ 29% inhibition at 1 mM Fusarium oxysporum
Hg2+ 94% inhibition at 1 mM Fusarium oxysporum
iodoacetic acid 10% inhibition at 1 mM Fusarium oxysporum
Mn2+ 7% inhibition at 1 mM Fusarium oxysporum
additional information no inhibition at 1 mM by Fe2+, Fe3+, Ba2+, Ca2+, and Mg2+, and by EDTA, o-phenanthroline, 2,2-diphenyl, and Tiron Fusarium oxysporum
Quinine 20% inhibition at 1 mM Fusarium oxysporum
Zn2+ 10% inhibition at 1 mM Fusarium oxysporum

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.28
-
choline pH 9.5, 25°C Fusarium oxysporum
0.39
-
Betaine aldehyde pH 9.5, 25°C Fusarium oxysporum

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
64000
-
2 * 64000, SDS-PAGE Fusarium oxysporum
128000
-
gel filtration Fusarium oxysporum

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
betaine aldehyde + O2 + H2O Fusarium oxysporum half-reaction betaine + H2O2
-
?
betaine aldehyde + O2 + H2O Fusarium oxysporum V2 half-reaction betaine + H2O2
-
?
choline + 2 O2 + H2O Fusarium oxysporum
-
betaine + 2 H2O2
-
?
choline + 2 O2 + H2O Fusarium oxysporum V2
-
betaine + 2 H2O2
-
?
choline + O2 Fusarium oxysporum half-reaction betaine aldehyde + H2O2
-
?
choline + O2 Fusarium oxysporum V2 half-reaction betaine aldehyde + H2O2
-
?
additional information Fusarium oxysporum the enzyme shows activity with trimethylamino alcohols and dimethylamino alcohols, but the Km increases with the number of methyl groups on the ammonium head group. N-replaced choline analogues as substrates lead to decreased maximum reaction velocities. The enzyme from Fusarium oxysporum shows a high affinity for choline and betaine aldehyde ?
-
?
additional information Fusarium oxysporum V2 the enzyme shows activity with trimethylamino alcohols and dimethylamino alcohols, but the Km increases with the number of methyl groups on the ammonium head group. N-replaced choline analogues as substrates lead to decreased maximum reaction velocities. The enzyme from Fusarium oxysporum shows a high affinity for choline and betaine aldehyde ?
-
?

Organism

Organism UniProt Comment Textmining
Fusarium oxysporum
-
-
-
Fusarium oxysporum V2
-
-
-

Purification (Commentary)

Purification (Comment) Organism
native enzyme 41fold by ammonium sulfate fractionation, gel filtation, anion exchange and hydroxyapatite chromatgraphy, and again gel filtration Fusarium oxysporum

Source Tissue

Source Tissue Comment Organism Textmining
additional information strain V2 shows better growth at pH 8.0 than pH 10.2, maximum enzyme activity after 4-5 days Fusarium oxysporum
-

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
32.1
-
purified enzyme, pH 10.0, 25°C Fusarium oxysporum

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2-methylaminoethanol + 2 O2 + H2O
-
Fusarium oxysporum ? + 2 H2O2
-
?
3-(dimethylamino)propan-1-ol + 2 O2 + H2O
-
Fusarium oxysporum ? + 2 H2O2
-
?
3-trimethylamino-1-propanol + 2 O2 + H2O
-
Fusarium oxysporum 3-trimethylaminopropanoate + 2 H2O2
-
?
4-trimethylamino-1-butanol + 2 O2 + H2O
-
Fusarium oxysporum 4-trimethylaminobutanoate + 2 H2O2
-
?
betaine aldehyde + O2 + H2O half-reaction Fusarium oxysporum betaine + H2O2
-
?
betaine aldehyde + O2 + H2O half-reaction Fusarium oxysporum V2 betaine + H2O2
-
?
choline + 2 O2 + H2O
-
Fusarium oxysporum betaine + 2 H2O2
-
?
choline + 2 O2 + H2O
-
Fusarium oxysporum V2 betaine + 2 H2O2
-
?
choline + O2 half-reaction Fusarium oxysporum betaine aldehyde + H2O2
-
?
choline + O2 half-reaction Fusarium oxysporum V2 betaine aldehyde + H2O2
-
?
diethylamino ethanol + 2 O2 + H2O2
-
Fusarium oxysporum ? + 2 H2O2
-
?
diethylaminoethanol + 2 O2 + H2O
-
Fusarium oxysporum ? + 2 H2O2
-
?
dimethylaminoethanol + 2 O2 + H2O
-
Fusarium oxysporum ? + 2 H2O2
-
?
additional information the enzyme shows activity with trimethylamino alcohols and dimethylamino alcohols, but the Km increases with the number of methyl groups on the ammonium head group. N-replaced choline analogues as substrates lead to decreased maximum reaction velocities. The enzyme from Fusarium oxysporum shows a high affinity for choline and betaine aldehyde Fusarium oxysporum ?
-
?
additional information no activity with betaine, monoethanolamine, triethanolamine, dimethylamino-1-butanol, N,N-dimethylglycine methylester, beta-methylcholine, L-carnitine, propanol, ethanol, and methanol Fusarium oxysporum ?
-
?
additional information the enzyme shows activity with trimethylamino alcohols and dimethylamino alcohols, but the Km increases with the number of methyl groups on the ammonium head group. N-replaced choline analogues as substrates lead to decreased maximum reaction velocities. The enzyme from Fusarium oxysporum shows a high affinity for choline and betaine aldehyde Fusarium oxysporum V2 ?
-
?
additional information no activity with betaine, monoethanolamine, triethanolamine, dimethylamino-1-butanol, N,N-dimethylglycine methylester, beta-methylcholine, L-carnitine, propanol, ethanol, and methanol Fusarium oxysporum V2 ?
-
?

Subunits

Subunits Comment Organism
homodimer 2 * 64000, SDS-PAGE Fusarium oxysporum

Synonyms

Synonyms Comment Organism
alkaliphilic choline oxidase
-
Fusarium oxysporum

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
35 40
-
Fusarium oxysporum

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
9.5
-
assay at Fusarium oxysporum

pH Range

pH Minimum pH Maximum Comment Organism
9 10
-
Fusarium oxysporum

pH Stability

pH Stability pH Stability Maximum Comment Organism
8 10.2 purified enzyme, 30°C, 10 min, stable at Fusarium oxysporum