Inhibitors | Comment | Organism | Structure |
---|---|---|---|
4-chloromercuribenzoate | 75% inhibition at 1 mM | Fusarium oxysporum | |
Cu2+ | 29% inhibition at 1 mM | Fusarium oxysporum | |
Hg2+ | 94% inhibition at 1 mM | Fusarium oxysporum | |
iodoacetic acid | 10% inhibition at 1 mM | Fusarium oxysporum | |
Mn2+ | 7% inhibition at 1 mM | Fusarium oxysporum | |
additional information | no inhibition at 1 mM by Fe2+, Fe3+, Ba2+, Ca2+, and Mg2+, and by EDTA, o-phenanthroline, 2,2-diphenyl, and Tiron | Fusarium oxysporum | |
Quinine | 20% inhibition at 1 mM | Fusarium oxysporum | |
Zn2+ | 10% inhibition at 1 mM | Fusarium oxysporum |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.28 | - |
choline | pH 9.5, 25°C | Fusarium oxysporum | |
0.39 | - |
Betaine aldehyde | pH 9.5, 25°C | Fusarium oxysporum |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
64000 | - |
2 * 64000, SDS-PAGE | Fusarium oxysporum |
128000 | - |
gel filtration | Fusarium oxysporum |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
betaine aldehyde + O2 + H2O | Fusarium oxysporum | half-reaction | betaine + H2O2 | - |
? | |
betaine aldehyde + O2 + H2O | Fusarium oxysporum V2 | half-reaction | betaine + H2O2 | - |
? | |
choline + 2 O2 + H2O | Fusarium oxysporum | - |
betaine + 2 H2O2 | - |
? | |
choline + 2 O2 + H2O | Fusarium oxysporum V2 | - |
betaine + 2 H2O2 | - |
? | |
choline + O2 | Fusarium oxysporum | half-reaction | betaine aldehyde + H2O2 | - |
? | |
choline + O2 | Fusarium oxysporum V2 | half-reaction | betaine aldehyde + H2O2 | - |
? | |
additional information | Fusarium oxysporum | the enzyme shows activity with trimethylamino alcohols and dimethylamino alcohols, but the Km increases with the number of methyl groups on the ammonium head group. N-replaced choline analogues as substrates lead to decreased maximum reaction velocities. The enzyme from Fusarium oxysporum shows a high affinity for choline and betaine aldehyde | ? | - |
? | |
additional information | Fusarium oxysporum V2 | the enzyme shows activity with trimethylamino alcohols and dimethylamino alcohols, but the Km increases with the number of methyl groups on the ammonium head group. N-replaced choline analogues as substrates lead to decreased maximum reaction velocities. The enzyme from Fusarium oxysporum shows a high affinity for choline and betaine aldehyde | ? | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Fusarium oxysporum | - |
- |
- |
Fusarium oxysporum V2 | - |
- |
- |
Purification (Comment) | Organism |
---|---|
native enzyme 41fold by ammonium sulfate fractionation, gel filtation, anion exchange and hydroxyapatite chromatgraphy, and again gel filtration | Fusarium oxysporum |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
additional information | strain V2 shows better growth at pH 8.0 than pH 10.2, maximum enzyme activity after 4-5 days | Fusarium oxysporum | - |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
32.1 | - |
purified enzyme, pH 10.0, 25°C | Fusarium oxysporum |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
2-methylaminoethanol + 2 O2 + H2O | - |
Fusarium oxysporum | ? + 2 H2O2 | - |
? | |
3-(dimethylamino)propan-1-ol + 2 O2 + H2O | - |
Fusarium oxysporum | ? + 2 H2O2 | - |
? | |
3-trimethylamino-1-propanol + 2 O2 + H2O | - |
Fusarium oxysporum | 3-trimethylaminopropanoate + 2 H2O2 | - |
? | |
4-trimethylamino-1-butanol + 2 O2 + H2O | - |
Fusarium oxysporum | 4-trimethylaminobutanoate + 2 H2O2 | - |
? | |
betaine aldehyde + O2 + H2O | half-reaction | Fusarium oxysporum | betaine + H2O2 | - |
? | |
betaine aldehyde + O2 + H2O | half-reaction | Fusarium oxysporum V2 | betaine + H2O2 | - |
? | |
choline + 2 O2 + H2O | - |
Fusarium oxysporum | betaine + 2 H2O2 | - |
? | |
choline + 2 O2 + H2O | - |
Fusarium oxysporum V2 | betaine + 2 H2O2 | - |
? | |
choline + O2 | half-reaction | Fusarium oxysporum | betaine aldehyde + H2O2 | - |
? | |
choline + O2 | half-reaction | Fusarium oxysporum V2 | betaine aldehyde + H2O2 | - |
? | |
diethylamino ethanol + 2 O2 + H2O2 | - |
Fusarium oxysporum | ? + 2 H2O2 | - |
? | |
diethylaminoethanol + 2 O2 + H2O | - |
Fusarium oxysporum | ? + 2 H2O2 | - |
? | |
dimethylaminoethanol + 2 O2 + H2O | - |
Fusarium oxysporum | ? + 2 H2O2 | - |
? | |
additional information | the enzyme shows activity with trimethylamino alcohols and dimethylamino alcohols, but the Km increases with the number of methyl groups on the ammonium head group. N-replaced choline analogues as substrates lead to decreased maximum reaction velocities. The enzyme from Fusarium oxysporum shows a high affinity for choline and betaine aldehyde | Fusarium oxysporum | ? | - |
? | |
additional information | no activity with betaine, monoethanolamine, triethanolamine, dimethylamino-1-butanol, N,N-dimethylglycine methylester, beta-methylcholine, L-carnitine, propanol, ethanol, and methanol | Fusarium oxysporum | ? | - |
? | |
additional information | the enzyme shows activity with trimethylamino alcohols and dimethylamino alcohols, but the Km increases with the number of methyl groups on the ammonium head group. N-replaced choline analogues as substrates lead to decreased maximum reaction velocities. The enzyme from Fusarium oxysporum shows a high affinity for choline and betaine aldehyde | Fusarium oxysporum V2 | ? | - |
? | |
additional information | no activity with betaine, monoethanolamine, triethanolamine, dimethylamino-1-butanol, N,N-dimethylglycine methylester, beta-methylcholine, L-carnitine, propanol, ethanol, and methanol | Fusarium oxysporum V2 | ? | - |
? |
Subunits | Comment | Organism |
---|---|---|
homodimer | 2 * 64000, SDS-PAGE | Fusarium oxysporum |
Synonyms | Comment | Organism |
---|---|---|
alkaliphilic choline oxidase | - |
Fusarium oxysporum |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
35 | 40 | - |
Fusarium oxysporum |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
9.5 | - |
assay at | Fusarium oxysporum |
pH Minimum | pH Maximum | Comment | Organism |
---|---|---|---|
9 | 10 | - |
Fusarium oxysporum |
pH Stability | pH Stability Maximum | Comment | Organism |
---|---|---|---|
8 | 10.2 | purified enzyme, 30°C, 10 min, stable at | Fusarium oxysporum |