Cloned (Comment) | Organism |
---|---|
recombinant expression in Escherichia coli | Arthrobacter globiformis |
Protein Variants | Comment | Organism |
---|---|---|
V464A | mutant, analysis of kinetic parameters | Arthrobacter globiformis |
V464T | mutant, analysis of kinetic parameters | Arthrobacter globiformis |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Arthrobacter globiformis | Q7X2H8 | - |
- |
Renatured (Comment) | Organism |
---|---|
fully oxidized form of the enzyme is obtained via slow oxidation of the flavosemiquinone by extensive dialysis at pH 6 and 4°C | Arthrobacter globiformis |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
0.3 | - |
V464A mutant, substrate choline (10 mM), pH 7 | Arthrobacter globiformis |
0.3 | - |
V464T mutant, substrate choline (10 mM), pH 7 | Arthrobacter globiformis |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
choline + O2 | - |
Arthrobacter globiformis | betaine aldehyde + H2O2 | - |
? |
Synonyms | Comment | Organism |
---|---|---|
choline oxidase | - |
Arthrobacter globiformis |
pH Stability | pH Stability Maximum | Comment | Organism |
---|---|---|---|
additional information | - |
comparison of pka values of the mutants V464A and V464T | Arthrobacter globiformis |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
FAD | covalently bound in both mutants (V464A and V464T) | Arthrobacter globiformis |