Any feedback?
Please rate this page
(literature.php)
(0/150)

BRENDA support

Literature summary for 1.1.3.15 extracted from

  • Eprintsev, A.; Semenov, A.; Navid, M.; Popov, V.
    Physical, chemical, and regulatory properties of glycolate oxidase in C3 and C4 plants (2009), Russ. J. Plant Physiol., 56, 164-167.
No PubMed abstract available

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
additional information
-
additional information Michaelis-Menten kinetics Zea mays
additional information
-
additional information Michaelis-Menten kinetics Glycine max
0.02
-
glycolate mesophyll cell enzyme Zea mays
0.056
-
glycolate bundle sheath cell enzyme Zea mays
0.06
-
glycolate leaf enzyme Glycine max

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
44000
-
2 x 37000 + 2 * 44000, SDS-PAGE Zea mays
160000
-
sequence calculation Zea mays

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
glycolate + O2 Zea mays
-
glyoxylate + H2O2
-
?
glycolate + O2 Glycine max
-
glyoxylate + H2O2
-
?

Organism

Organism UniProt Comment Textmining
Glycine max
-
cv. Pripyat, various isozymes
-
Zea mays
-
cv. Voronezhskaya 76, various isozymes
-

Purification (Commentary)

Purification (Comment) Organism
native enzyme from leaves 207fold by ammonium sulfate fractionation, gel filtration, anion exchange chromatography, and again gel filtration Glycine max
native enzyme from maize leaf bundle sheath 46.6fold by gel filtration, anion exchange chromatography, and again gel filtration. Native enzyme 10.5fold from leaf mesophyll, by ammonium sulfate fractionation, gel filtration, anion exchange chromatography, and again gel filtration Zea mays

Source Tissue

Source Tissue Comment Organism Textmining
bundle sheath
-
Zea mays
-
leaf
-
Zea mays
-
leaf
-
Glycine max
-
mesophyll
-
Zea mays
-

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
0.65
-
purified native enzyme from leaves Zea mays
1546
-
purified native enzyme from mesophyll bundle sheath Zea mays

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
glycolate + O2
-
Zea mays glyoxylate + H2O2
-
?
glycolate + O2
-
Glycine max glyoxylate + H2O2
-
?

Subunits

Subunits Comment Organism
More quaternary structure of C3 plant GO, overview Zea mays
octamer
-
Glycine max
tetramer 2 x 37000 + 2 * 44000, SDS-PAGE Zea mays

Synonyms

Synonyms Comment Organism
glycolate oxidase
-
Zea mays
glycolate oxidase
-
Glycine max

pH Range

pH Minimum pH Maximum Comment Organism
additional information
-
the dependence of GO activity on pH is bell-shaped Zea mays

General Information

General Information Comment Organism
metabolism the enzyme is involved in the metabolism of glycolate Glycine max
metabolism the enzyme is involved in the metabolism of glycolate, glycolate oxidase is a key enzyme involved in C3 photorespiration metabolic pathway, the process where plants lose up to half of assimilated carbon Zea mays
physiological function role in C3 and C4 plants and associated regulation mechanisms Zea mays
physiological function role in C3 and C4 plants and associated regulation mechanisms Glycine max