Inhibitors | Comment | Organism | Structure |
---|---|---|---|
2-oxobutyrate | non-competitive inhibition at 5 mM | Rattus sp. | |
2-oxoisocaproate | non-competitive inhibition at 5 mM, most active inhibitor of 2-keto acids, oxidation of 2-hydroxybutyrate most sensitive | Rattus sp. | |
2-oxoisovalerate | non-competitive inhibition at 5 mM | Rattus sp. | |
2-oxovalerate | non-competitive inhibition at 5 mM | Rattus sp. | |
DL-2-hydroxyisocaproate | marked inhibition above 50 mM | Rattus sp. | |
DL-2-hydroxyvalerate | marked inhibition above 50 mM | Rattus sp. |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.6 | - |
DL-2-hydroxyvalerate | - |
Rattus sp. | |
0.6 | - |
DL-2-hydroxyisocaproate | - |
Rattus sp. | |
0.6 | - |
DL-2-hydroxyisovalerate | - |
Rattus sp. | |
0.8 | 0.9 | DL-2-hydroxycaproate | - |
Rattus sp. | |
2.4 | 2.8 | DL-2-hydroxybutyrate | - |
Rattus sp. | |
5.6 | 5.7 | L-lactate | - |
Rattus sp. |
Localization | Comment | Organism | GeneOntology No. | Textmining |
---|---|---|---|---|
cytosol | - |
Rattus sp. | 5829 | - |
mitochondrion | preparation of heavy and light mitochondria, 2 distinct molecular forms | Rattus sp. | 5739 | - |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
DL-2-hydroxybutyrate + O2 | Rattus sp. | higher affinity with C5 and C6 hydroxy acids | 2-oxobutyrate + H2O2 | - |
? | |
DL-2-hydroxycaproate + O2 | Rattus sp. | - |
2-oxocaproate + H2O2 | - |
? | |
DL-2-hydroxyisocaproate + O2 | Rattus sp. | - |
2-oxoisocaproate + H2O2 | - |
? | |
DL-2-hydroxyisovalerate + O2 | Rattus sp. | low activity | 2-oxoisovalerate + H2O2 | - |
? | |
DL-2-hydroxyvalerate + O2 | Rattus sp. | - |
2-oxovalerate + H2O2 | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Rattus sp. | - |
- |
- |
Purification (Comment) | Organism |
---|---|
to homogeneity, chromatography steps | Rattus sp. |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
kidney | - |
Rattus sp. | - |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
DL-2-hydroxybutyrate + O2 | higher affinity with C5 and C6 hydroxy acids | Rattus sp. | 2-oxobutyrate + H2O2 | - |
? | |
DL-2-hydroxycaproate + O2 | - |
Rattus sp. | 2-oxocaproate + H2O2 | - |
? | |
DL-2-hydroxyisocaproate + O2 | - |
Rattus sp. | 2-oxoisocaproate + H2O2 | - |
? | |
DL-2-hydroxyisovalerate + O2 | low activity | Rattus sp. | 2-oxoisovalerate + H2O2 | - |
? | |
DL-2-hydroxyvalerate + O2 | - |
Rattus sp. | 2-oxovalerate + H2O2 | - |
? | |
L-lactate + 2,6-dichlorophenolindophenol | lower activity for preparation from "heavy" mitochondria | Rattus sp. | pyruvate + reduced 2,6-dichlorophenolindophenol | - |
? |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
8.6 | 8.9 | substrate: DL-2-hydroxybutyrate | Rattus sp. |
8.9 | 9.4 | substrate: DL-2-hydroxyisocaproate | Rattus sp. |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
FMN | - |
Rattus sp. |