Cloned (Comment) | Organism |
---|---|
expression in Escherichia coli | Rattus sp. |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
oxalate | competitive inhibition | Rattus sp. | |
pyruvate | competitive inhibition | Rattus sp. | |
trans-cinnamate | competitive inhibition | Rattus sp. |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.09 | - |
L-Phenyllactate | isozyme beta1 | Rattus sp. | |
0.13 | - |
L-Phenyllactate | isozyme beta2 | Rattus sp. | |
0.16 | - |
L-Mandelate | isozyme beta1 | Rattus sp. | |
0.23 | - |
L-Mandelate | isozyme beta2 | Rattus sp. | |
0.3 | - |
L-2-Hydroxyisocaproate | isozyme beta1 | Rattus sp. | |
0.32 | - |
L-2-Hydroxyisocaproate | isozyme beta2 | Rattus sp. | |
0.7 | - |
DL-2-hydroxy-4-methylthiobutanoic acid | - |
Rattus sp. | |
1.1 | - |
DL-2-hydroxy-4-methylthiobutanoic acid | isozyme beta2 | Rattus sp. | |
2.04 | - |
DL-2-hydroxybutyrate | isozyme beta2 | Rattus sp. | |
2.5 | - |
DL-2-hydroxybutyrate | isozyme beta1 | Rattus sp. | |
4.7 | - |
L-lactate | isozyme beta2 | Rattus sp. | |
5.3 | - |
L-leucine | isozyme beta2 | Rattus sp. | |
6.1 | - |
L-lactate | isozyme beta1 | Rattus sp. | |
6.4 | - |
L-leucine | isozyme beta1 | Rattus sp. |
Localization | Comment | Organism | GeneOntology No. | Textmining |
---|---|---|---|---|
peroxisome | - |
Rattus sp. | 5777 | - |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Rattus sp. | - |
- |
- |
Purification (Comment) | Organism |
---|---|
recombinant isozymes beta1, beta2 | Rattus sp. |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
0.78 | - |
isozyme beta1 | Rattus sp. |
Storage Stability | Organism |
---|---|
4°C, 60% ammonium sulfate precipitate, several months | Rattus sp. |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
DL-2-hydroxy-4-methylthiobutanoic acid + 2,6-dichlorophenolindophenol | - |
Rattus sp. | 2-oxo-4-methylthiobutanoic acid + reduced 2,6-dichlorophenolindophenol | - |
? | |
DL-2-hydroxybutyrate + 2,6-dichlorophenolindophenol | - |
Rattus sp. | 2-oxobutyrate + reduced 2,6-dichlorophenolindophenol | - |
? | |
L-2-hydroxyisocaproate + 2,6-dichlorophenolindophenol | - |
Rattus sp. | 2-oxoisocaproate + reduced 2,6-dichlorophenolindophenol | - |
? | |
L-lactate + 2,6-dichlorophenolindophenol | - |
Rattus sp. | pyruvate + reduced 2,6-dichlorophenolindophenol | - |
? | |
L-leucine + 2,6-dichlorophenolindophenol | - |
Rattus sp. | ? + reduced 2,6-dichlorophenolindophenol | - |
? | |
L-mandelate + 2,6-dichlorophenolindophenol | - |
Rattus sp. | oxo(phenyl)acetic acid + reduced 2,6-dichlorophenolindophenol | - |
? | |
L-phenyllactate + 2,6-dichlorophenolindophenol | - |
Rattus sp. | phenylpyruvate + reduced 2,6-dichlorophenolindophenol | - |
? |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.014 | - |
L-leucine | isozymes beta1 and beta2 | Rattus sp. | |
0.26 | - |
L-lactate | isozyme beta1 | Rattus sp. | |
0.38 | - |
L-lactate | isozyme beta2 | Rattus sp. | |
0.59 | - |
L-Phenyllactate | isozyme beta1 | Rattus sp. | |
0.7 | - |
L-Phenyllactate | isozyme beta2 | Rattus sp. | |
1.55 | - |
L-Mandelate | isozyme beta1 | Rattus sp. | |
1.66 | - |
DL-2-hydroxybutyrate | isozyme beta1 | Rattus sp. | |
1.9 | - |
DL-2-hydroxy-4-methylthiobutanoic acid | isozyme beta2 | Rattus sp. | |
2 | - |
L-2-Hydroxyisocaproate | isozyme beta1 | Rattus sp. | |
2 | - |
DL-2-hydroxybutyrate | isozyme beta2 | Rattus sp. | |
2.1 | - |
DL-2-hydroxy-4-methylthiobutanoic acid | isozyme beta1 | Rattus sp. | |
2.4 | - |
L-2-Hydroxyisocaproate | isozyme beta2 | Rattus sp. | |
2.51 | - |
L-Mandelate | isozyme beta2 | Rattus sp. |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
FMN | three-residue insertion of loop 4 in isozyme beta2 influences ionisation state of FMN | Rattus sp. |
Ki Value [mM] | Ki Value maximum [mM] | Inhibitor | Comment | Organism | Structure |
---|---|---|---|---|---|
1 | - |
trans-cinnamate | isozyme beta1 | Rattus sp. | |
1.9 | - |
trans-cinnamate | isozyme beta2 | Rattus sp. | |
10.5 | - |
oxalate | isozyme beta1 | Rattus sp. | |
15.9 | - |
pyruvate | isozyme beta1 | Rattus sp. | |
18.3 | - |
pyruvate | isozyme beta2 | Rattus sp. | |
23 | - |
oxalate | isozyme beta2 | Rattus sp. |