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Literature summary for 1.1.3.13 extracted from
- Efficient expression and purification of recombinant alcohol oxidase in Pichia pastoris (2012), Biotechnol. Bioprocess Eng., 17, 693-702.
No PubMed abstract available
Cloned(Commentary)
| Cloned (Comment) | Organism |
|---|---|
| functional recombinant expression of C-terminally His6-tagged enzyme in Pichia pastoris strain GS115 from the plasmid pPIC9K-AOX intracellularly and extracellularly, batch culture. Expression in Escherichia coli strain DH5alpha in batch culture. Method comparison and optimization, overview | Komagataella pastoris |
Inhibitors
| Inhibitors | Comment | Organism | Structure |
|---|---|---|---|
| H2O2 | inhibits at 5 mM, at 7.8 mM the enzyme maintains 50% of its initial activity after 0.5 h of incubation at 4°C | Komagataella pastoris |  |
Organic Solvent Stability
| Organic Solvent | Comment | Organism |
|---|---|---|
| Ethanol | pH 8.0, activates at 30% v/v for 0.5 h, then inhibits after 8 h | Komagataella pastoris |
| hexanol | pH 8.0, activates at 30% v/v for 0.5 h, then inhibits slightly after 8 h | Komagataella pastoris |
| isoamyl alcohol | pH 8.0, activates at 30% v/v for 0.5 h, then inhibits after 8 h | Komagataella pastoris |
| isobutanol | pH 8.0, only slight effect on enzyme activity at 30% v/v | Komagataella pastoris |
| isopropanol | pH 8.0, activates at 30% v/v for 3 h, then inhibits strongly up after h | Komagataella pastoris |
| Methanol | pH 8.0, activates at 30% v/v for 0.5 h, then inhibits after 8 h | Komagataella pastoris |
| additional information | effects of various solvents on the stability and activity of recmbinant AOX, overview | Komagataella pastoris |
| n-Butanol | pH 8.0, activates at 30% v/v for 0.5 h, then inhibits after 8 h | Komagataella pastoris |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Komagataella pastoris | - |
- |
- |
| Komagataella pastoris GS115 | - |
- |
- |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
| recombinant C-terminally His6-tagged enzyme from Pichia pastoris strain GS115 by nickel affinity chromatography and gel filtration. The recombinant enzyme shows high thermal stability and resistance to H2O2 inhibition | Komagataella pastoris |
Specific Activity [micromol/min/mg]
| Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|
| 11.85 | - |
purified recombinant intracellular enzyme, pH 80, 25°C | Komagataella pastoris |
| 12.94 | - |
purified recombinant extracellular enzyme, pH 80, 25°C | Komagataella pastoris |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| 1,2-propanediol + O2 | - |
Komagataella pastoris | ? + H2O2 | - |
? | |
| 1,3-butylene glycol + O2 | - |
Komagataella pastoris | ? + H2O2 | - |
? | |
| 1,3-butylene glycol + O2 | - |
Komagataella pastoris GS115 | ? + H2O2 | - |
? | |
| 2-propanol + O2 | - |
Komagataella pastoris | 2-oxopropane + H2O2 | - |
? | |
| ethanol + O2 | - |
Komagataella pastoris | acetaldehyde + H2O2 | - |
? | |
| ethanol + O2 | - |
Komagataella pastoris GS115 | acetaldehyde + H2O2 | - |
? | |
| hexanol + O2 | - |
Komagataella pastoris | hexanal + H2O2 | - |
? | |
| hexanol + O2 | - |
Komagataella pastoris GS115 | hexanal + H2O2 | - |
? | |
| methanol + O2 | - |
Komagataella pastoris | formaldehyde + H2O2 | - |
? | |
| methanol + O2 | - |
Komagataella pastoris GS115 | formaldehyde + H2O2 | - |
? | |
| n-butanol + O2 | - |
Komagataella pastoris | butanal + H2O2 | - |
? | |
| n-butanol + O2 | - |
Komagataella pastoris GS115 | butanal + H2O2 | - |
? | |
Synonyms
| Synonyms | Comment | Organism |
|---|---|---|
| AOX | - |
Komagataella pastoris |
Temperature Optimum [°C]
| Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 37 | - |
- |
Komagataella pastoris |
Temperature Stability [°C]
| Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 42 | - |
purified recombinant His-tagged enzyme, pH 8.0, 60 min, stable | Komagataella pastoris |
| 50 | - |
purified recombinant His-tagged enzyme, pH 8.0, 50 min, 50% activity remaining | Komagataella pastoris |
| 55 | - |
purified recombinant His-tagged enzyme, pH 8.0, 30 min, inactivation | Komagataella pastoris |
pH Stability
| pH Stability | pH Stability Maximum | Comment | Organism |
|---|---|---|---|
| 4.2 | - |
purified recombinant His-tagged enzyme, 25°C, 3 h, inactivation | Komagataella pastoris |
| 5.5 | 7 | purified recombinant His-tagged enzyme, 25°C, 3 h, 50% activity remaining | Komagataella pastoris |
| 8 | 10 | purified recombinant His-tagged enzyme, 25°C, 3 h, stable | Komagataella pastoris |
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