Literature summary for 1.1.3.13 extracted from

Lopez-Gallego, F.; Betancor, L.; Hidalgo, A.; Dellamora-Ortiz, G.; Mateo, C.; Fernandez-Lafuente, R.; Guisan, J.M.
Stabilization of different alcohol oxidases via immobilization and post immobilization techniques (2007), Enzyme Microb. Technol., 40, 278-284.

No PubMed abstract available

General Stability

General Stability	Organism
stabilizing immobilization by ionic adsorption on agarose coated with 600 kDa polyethylenimine. The adsorption of the proteins on the polymeric bed allows the stabilization of the quaternary structure. The effect of the enzyme concentration on thermal enzyme stability disappears in these immobilized preparations. A high stability is obtained without a significant decrease in enzyme activity during immobilization (recovered activity was over 50%)	Komagataella pastoris
stabilizing immobilization by ionic adsorption on agarose coated with 600 kDa polyethylenimine. The adsorption of the proteins on the polymeric bed allows the stabilization of the quaternary structure. The effect of the enzyme concentration on thermal enzyme stability disappears in these immobilized preparations. A high stability is obtained without a significant decrease in enzyme activity during immobilization (recovered activity was over 50%)	Hansenula sp.

Organism

stabilizing immobilization by ionic adsorption on agarose coated with 600 kDa polyethylenimine. The adsorption of the proteins on the polymeric bed allows the stabilization of the quaternary structure. The effect of the enzyme concentration on thermal enzyme stability disappears in these immobilized preparations. A high stability is obtained without a significant decrease in enzyme activity during immobilization (recovered activity was over 50%)

Komagataella pastoris

Hansenula sp.

Organism

Organism	UniProt	Comment	Textmining
Hansenula sp.	-	-	-
Komagataella pastoris	-	-	-
[Candida] boidinii	-	-	-

Temperature Stability [°C]

Temperature Stability Minimum [°C]	Temperature Stability Maximum [°C]	Comment	Organism
45	-	pH 7, 10 mM sodium phosphate, 1 h, 90% loss of activity	[Candida] boidinii
45	-	pH 7, 10 mM sodium phosphate, about 40% loss of activity after 3 h, about 40% loss of activity after 6 h, about 70% loss of activity after 23 h. Stabilizing immobilization by ionic adsorption on agarose coated with 600 kDa polyethylenimine. The adsorption of the proteins on the polymeric bed allows the stabilization of the quaternary structure. The effect of the enzyme concentration on thermal enzyme stability disappears in these immobilized preparations. A high stability is obtained without a significant decrease in enzyme activity during immobilization (recovered activity was over 50%)	Hansenula sp.
45	-	pH 7, 10 mM sodium phosphate, about 50% loss of activity after 3 h, about 60% loss of activity after 6 h, about 80% loss of activity after 23 h. Stabilizing immobilization by ionic adsorption on agarose coated with 600 kDa polyethylenimine. The adsorption of the proteins on the polymeric bed allows the stabilization of the quaternary structure. The effect of the enzyme concentration on thermal enzyme stability disappears in these immobilized preparations. A high stability is obtained without a significant decrease in enzyme activity during immobilization (recovered activity was over 50%)	Komagataella pastoris