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Literature summary for 1.1.3.13 extracted from

  • Ko, H.S.; Yokoyama, Y.; Ohno, N.; Okadome, M.; Amachi, S.; Shinoyama, H.; Fujii, T.
    Purification and characterization of intracellular and extracellular, thermostable and alkali-tolerant alcohol oxidases produced by a thermophilic fungus, Thermoascus aurantiacus NBRC 31693 (2005), J. Biosci. Bioeng., 99, 348-353.
    View publication on PubMed

Inhibitors

Inhibitors Comment Organism Structure
EDTA slight inhibition of intra- and extracellular enzymes Thermoascus aurantiacus
Fe2+ slight inhibition of extracellular enzyme Thermoascus aurantiacus
Hg2+ strong inhibition of intra- and extracellular enzymes Thermoascus aurantiacus
K+ slight inhibition of extracellular enzyme Thermoascus aurantiacus
KCN inhibition of intra- and extracellular enzymes Thermoascus aurantiacus
Mn2+ slight inhibition of intra- and extracellular enzymes Thermoascus aurantiacus
Na+ slight inhibition of extracellular enzyme Thermoascus aurantiacus
NaN3 inhibition of intra- and extracellular enzymes Thermoascus aurantiacus
NEM slight inhibition of intra- and extracellular enzymes Thermoascus aurantiacus

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.5
-
methanol extracellular enzyme, pH 5.9, 30°C Thermoascus aurantiacus
10.2
-
ethanol extracellular enzyme, pH 5.9, 30°C Thermoascus aurantiacus
13.5
-
methanol intracellular enzyme, pH 5.9, 30°C Thermoascus aurantiacus
15.8
-
ethanol intracellular enzyme, pH 5.9, 30°C Thermoascus aurantiacus

Metals/Ions

Metals/Ions Comment Organism Structure
K+ slight activation of intracellular enzyme Thermoascus aurantiacus

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
320000
-
intracellular enzyme, gel filtration Thermoascus aurantiacus
560000
-
extracellular enzyme, gel filtration Thermoascus aurantiacus

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
ethanol + O2 Thermoascus aurantiacus
-
acetaldehyde + H2O2
-
?
methanol + O2 Thermoascus aurantiacus
-
formaldehyde + H2O2
-
?
methanol + O2 Thermoascus aurantiacus NBRC 31693
-
formaldehyde + H2O2
-
?

Organism

Organism UniProt Comment Textmining
Thermoascus aurantiacus
-
thermophilic fungus
-
Thermoascus aurantiacus NBRC 31693
-
thermophilic fungus
-

Purification (Commentary)

Purification (Comment) Organism
native intracellular enzyme, 9.24fold, and extracellular enzyme, 314fol, from strain NBRC 31693, by streptomycin precipitation, dialysis, anion exchange chromatography and gel filtration or by polethylene glycol precipitation, anion exchange and hydrophobic interaction chromatography, and gel filtration, respectively Thermoascus aurantiacus

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
1.67
-
purified intracellular enzyme Thermoascus aurantiacus
9.42
-
purified extracellular enzyme Thermoascus aurantiacus

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1-butanol + O2 intra- and extracellular enzymes, low activity with the intracellular enzyme Thermoascus aurantiacus butanaldehyde + H2O2
-
?
1-pentanol + O2 intra- and extracellular enzymes, low activity with the intracellular enzyme Thermoascus aurantiacus pentanaldehyde + H2O2
-
?
2-methyl-2-propanol + O2 extracellular enzyme, no activity with the intracellular enzyme Thermoascus aurantiacus ?
-
?
2-propanol + O2 low activity with the extracellular enzyme Thermoascus aurantiacus acetone + H2O2
-
?
2-propanol + O2 low activity with the extracellular enzyme Thermoascus aurantiacus NBRC 31693 acetone + H2O2
-
?
arabitol + O2 extracellular enzyme, no activity with the intracellular enzyme Thermoascus aurantiacus ?
-
?
benzyl alcohol + O2 extracellular enzyme, no activity with the intracellular enzyme Thermoascus aurantiacus benzaldehyde + H2O2
-
?
benzyl alcohol + O2 extracellular enzyme, no activity with the intracellular enzyme Thermoascus aurantiacus NBRC 31693 benzaldehyde + H2O2
-
?
ethanol + O2
-
Thermoascus aurantiacus acetaldehyde + H2O2
-
?
ethanol + O2 intra- and extracellular enzymes Thermoascus aurantiacus acetaldehyde + H2O2
-
?
ethylene glycol + O2 intra- and extracellular enzymes, low activity with the intracellular enzyme Thermoascus aurantiacus ?
-
?
methanol + O2
-
Thermoascus aurantiacus formaldehyde + H2O2
-
?
methanol + O2 intra- and extracellular enzymes Thermoascus aurantiacus formaldehyde + H2O2
-
?
methanol + O2
-
Thermoascus aurantiacus NBRC 31693 formaldehyde + H2O2
-
?
methanol + O2 intra- and extracellular enzymes Thermoascus aurantiacus NBRC 31693 formaldehyde + H2O2
-
?
additional information substrate specificities of intra- and extracellular enzymes, overview, no activity by both with 2-butanol, 2-methyl-1-propanol, and glycerol Thermoascus aurantiacus ?
-
?
additional information substrate specificities of intra- and extracellular enzymes, overview, no activity by both with 2-butanol, 2-methyl-1-propanol, and glycerol Thermoascus aurantiacus NBRC 31693 ?
-
?
n-propanol + O2 intra- and extracellular enzymes Thermoascus aurantiacus propionaldehyde + H2O2
-
?
propylene glycol + O2 low activity with intra- and extracellular enzymes Thermoascus aurantiacus ?
-
?
veratryl alcohol + O2 extracellular enzyme, no activity with the intracellular enzyme Thermoascus aurantiacus ?
-
?

Synonyms

Synonyms Comment Organism
AOext
-
Thermoascus aurantiacus
AOint
-
Thermoascus aurantiacus
extracellular alcohol oxidase
-
Thermoascus aurantiacus
intracellular alcohol oxidase
-
Thermoascus aurantiacus

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
30
-
assay at Thermoascus aurantiacus

Temperature Stability [°C]

Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
55
-
15 min, pH 8.0, purified extracellular enzyme, completely stable Thermoascus aurantiacus
60
-
15 min, pH 8.0, purified intracellular enzyme, completely stable Thermoascus aurantiacus

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
7.2
-
assay at Thermoascus aurantiacus

pH Stability

pH Stability pH Stability Maximum Comment Organism
5 11 purified intracellular enzyme, stable Thermoascus aurantiacus
6 11 purified extracellular enzyme, stable Thermoascus aurantiacus

pI Value

Organism Comment pI Value Maximum pI Value
Thermoascus aurantiacus purified intracellular enzyme
-
5.9
Thermoascus aurantiacus purified extracellular enzyme
-
6.1