Inhibitors | Comment | Organism | Structure |
---|---|---|---|
EDTA | slight inhibition of intra- and extracellular enzymes | Thermoascus aurantiacus | |
Fe2+ | slight inhibition of extracellular enzyme | Thermoascus aurantiacus | |
Hg2+ | strong inhibition of intra- and extracellular enzymes | Thermoascus aurantiacus | |
K+ | slight inhibition of extracellular enzyme | Thermoascus aurantiacus | |
KCN | inhibition of intra- and extracellular enzymes | Thermoascus aurantiacus | |
Mn2+ | slight inhibition of intra- and extracellular enzymes | Thermoascus aurantiacus | |
Na+ | slight inhibition of extracellular enzyme | Thermoascus aurantiacus | |
NaN3 | inhibition of intra- and extracellular enzymes | Thermoascus aurantiacus | |
NEM | slight inhibition of intra- and extracellular enzymes | Thermoascus aurantiacus |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.5 | - |
methanol | extracellular enzyme, pH 5.9, 30°C | Thermoascus aurantiacus | |
10.2 | - |
ethanol | extracellular enzyme, pH 5.9, 30°C | Thermoascus aurantiacus | |
13.5 | - |
methanol | intracellular enzyme, pH 5.9, 30°C | Thermoascus aurantiacus | |
15.8 | - |
ethanol | intracellular enzyme, pH 5.9, 30°C | Thermoascus aurantiacus |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
K+ | slight activation of intracellular enzyme | Thermoascus aurantiacus |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
320000 | - |
intracellular enzyme, gel filtration | Thermoascus aurantiacus |
560000 | - |
extracellular enzyme, gel filtration | Thermoascus aurantiacus |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
ethanol + O2 | Thermoascus aurantiacus | - |
acetaldehyde + H2O2 | - |
? | |
methanol + O2 | Thermoascus aurantiacus | - |
formaldehyde + H2O2 | - |
? | |
methanol + O2 | Thermoascus aurantiacus NBRC 31693 | - |
formaldehyde + H2O2 | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Thermoascus aurantiacus | - |
thermophilic fungus | - |
Thermoascus aurantiacus NBRC 31693 | - |
thermophilic fungus | - |
Purification (Comment) | Organism |
---|---|
native intracellular enzyme, 9.24fold, and extracellular enzyme, 314fol, from strain NBRC 31693, by streptomycin precipitation, dialysis, anion exchange chromatography and gel filtration or by polethylene glycol precipitation, anion exchange and hydrophobic interaction chromatography, and gel filtration, respectively | Thermoascus aurantiacus |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
1.67 | - |
purified intracellular enzyme | Thermoascus aurantiacus |
9.42 | - |
purified extracellular enzyme | Thermoascus aurantiacus |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
1-butanol + O2 | intra- and extracellular enzymes, low activity with the intracellular enzyme | Thermoascus aurantiacus | butanaldehyde + H2O2 | - |
? | |
1-pentanol + O2 | intra- and extracellular enzymes, low activity with the intracellular enzyme | Thermoascus aurantiacus | pentanaldehyde + H2O2 | - |
? | |
2-methyl-2-propanol + O2 | extracellular enzyme, no activity with the intracellular enzyme | Thermoascus aurantiacus | ? | - |
? | |
2-propanol + O2 | low activity with the extracellular enzyme | Thermoascus aurantiacus | acetone + H2O2 | - |
? | |
2-propanol + O2 | low activity with the extracellular enzyme | Thermoascus aurantiacus NBRC 31693 | acetone + H2O2 | - |
? | |
arabitol + O2 | extracellular enzyme, no activity with the intracellular enzyme | Thermoascus aurantiacus | ? | - |
? | |
benzyl alcohol + O2 | extracellular enzyme, no activity with the intracellular enzyme | Thermoascus aurantiacus | benzaldehyde + H2O2 | - |
? | |
benzyl alcohol + O2 | extracellular enzyme, no activity with the intracellular enzyme | Thermoascus aurantiacus NBRC 31693 | benzaldehyde + H2O2 | - |
? | |
ethanol + O2 | - |
Thermoascus aurantiacus | acetaldehyde + H2O2 | - |
? | |
ethanol + O2 | intra- and extracellular enzymes | Thermoascus aurantiacus | acetaldehyde + H2O2 | - |
? | |
ethylene glycol + O2 | intra- and extracellular enzymes, low activity with the intracellular enzyme | Thermoascus aurantiacus | ? | - |
? | |
methanol + O2 | - |
Thermoascus aurantiacus | formaldehyde + H2O2 | - |
? | |
methanol + O2 | intra- and extracellular enzymes | Thermoascus aurantiacus | formaldehyde + H2O2 | - |
? | |
methanol + O2 | - |
Thermoascus aurantiacus NBRC 31693 | formaldehyde + H2O2 | - |
? | |
methanol + O2 | intra- and extracellular enzymes | Thermoascus aurantiacus NBRC 31693 | formaldehyde + H2O2 | - |
? | |
additional information | substrate specificities of intra- and extracellular enzymes, overview, no activity by both with 2-butanol, 2-methyl-1-propanol, and glycerol | Thermoascus aurantiacus | ? | - |
? | |
additional information | substrate specificities of intra- and extracellular enzymes, overview, no activity by both with 2-butanol, 2-methyl-1-propanol, and glycerol | Thermoascus aurantiacus NBRC 31693 | ? | - |
? | |
n-propanol + O2 | intra- and extracellular enzymes | Thermoascus aurantiacus | propionaldehyde + H2O2 | - |
? | |
propylene glycol + O2 | low activity with intra- and extracellular enzymes | Thermoascus aurantiacus | ? | - |
? | |
veratryl alcohol + O2 | extracellular enzyme, no activity with the intracellular enzyme | Thermoascus aurantiacus | ? | - |
? |
Synonyms | Comment | Organism |
---|---|---|
AOext | - |
Thermoascus aurantiacus |
AOint | - |
Thermoascus aurantiacus |
extracellular alcohol oxidase | - |
Thermoascus aurantiacus |
intracellular alcohol oxidase | - |
Thermoascus aurantiacus |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
30 | - |
assay at | Thermoascus aurantiacus |
Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|
55 | - |
15 min, pH 8.0, purified extracellular enzyme, completely stable | Thermoascus aurantiacus |
60 | - |
15 min, pH 8.0, purified intracellular enzyme, completely stable | Thermoascus aurantiacus |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
7.2 | - |
assay at | Thermoascus aurantiacus |
pH Stability | pH Stability Maximum | Comment | Organism |
---|---|---|---|
5 | 11 | purified intracellular enzyme, stable | Thermoascus aurantiacus |
6 | 11 | purified extracellular enzyme, stable | Thermoascus aurantiacus |
Organism | Comment | pI Value Maximum | pI Value |
---|---|---|---|
Thermoascus aurantiacus | purified intracellular enzyme | - |
5.9 |
Thermoascus aurantiacus | purified extracellular enzyme | - |
6.1 |