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Literature summary for 1.1.3.13 extracted from

  • Janssen, F.W.; Ruelius, H.W.
    Alcohol oxidase, a flavoprotein from several Basidiomycetes species. Crystallization by fractional precipitation with polyethylene glycol (1968), Biochim. Biophys. Acta, 151, 330-342.
    View publication on PubMed
Search for more literature for 1.1.3.13

Application

Application Comment Organism
analysis enzyme may be useful for the colorimetric determination of methanol, ethanol, or other alcohols Basidiomycota

Crystallization (Commentary)

Crystallization (Comment) Organism
-
 Basidiomycota

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
1.52
-
 methanol
-
 Basidiomycota
10
-
 ethanol
-
 Basidiomycota
54.6
-
 n-Propanol
-
 Basidiomycota
133
-
 n-butanol
-
 Basidiomycota

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
300000
-
 above, gel filtration Basidiomycota

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
methanol + O2 Basidiomycota key enzyme of methanol metabolism formaldehyde + H2O2
-
 ?

Organism

Organism UniProt Comment Textmining
Basidiomycota
-
-
-

Posttranslational Modification

Posttranslational Modification Comment Organism
no glycoprotein
-
 Basidiomycota

Purification (Commentary)

Purification (Comment) Organism
-
 Basidiomycota

Source Tissue

Source Tissue Comment Organism Textmining
mycelium
-
 Basidiomycota
-

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
additional information
-
-
 Basidiomycota

Storage Stability

Storage Stability Organism
crystalline, stable for at least 5 months in 0.05 M phosphate buffer, pH 7.7 Basidiomycota

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1-pentanol + O2 1% of the activity with methanol Basidiomycota n-pentanal + H2O2
-
 ?
2-propen-1-ol + O2 17% of the activity with methanol Basidiomycota 2-propen-1-al + H2O2
-
 ?
2-propyn-1-ol + O2 45% of the activity with methanol Basidiomycota propyn-1-al + H2O2
-
 ?
bromoethanol + O2 4% of the activity with methanol Basidiomycota bromoethanal + H2O2
-
 ?
chloroethanol + O2 6% of the activity with methanol Basidiomycota chloroethanal + H2O2
-
 ?
chloroethanol + O2 6% of the activity with Basidiomycota chloroethanal + H2O2
-
 ?
ethanol + O2 28% of the activity with methanol Basidiomycota acetaldehyde + H2O2
-
 ?
methanol + O2
-
 Basidiomycota formaldehyde + H2O2
-
 ?
methanol + O2 key enzyme of methanol metabolism Basidiomycota formaldehyde + H2O2
-
 ?
n-butanol + O2 2.1% of the activity with methanol Basidiomycota n-butanal + H2O2
-
 ?
n-butanol + O2 2.1%, of the activity with methanol Basidiomycota n-butanal + H2O2
-
 ?
n-propanol + O2 5.3% of the activity with methanol Basidiomycota n-propanal + H2O2
-
 ?

Temperature Stability [°C]

Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
25
-
 pH 5.0, 3 h, complete inactivation at pH 5.0 after 3 h Basidiomycota

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
6.3 9
-
 Basidiomycota
6.5 9
-
 Basidiomycota

pH Stability

pH Stability pH Stability Maximum Comment Organism
5
-
 25°C, 3 h, complete inactivation Basidiomycota
6.5
-
 rapid inactivation below Basidiomycota
7 9 25°C, 3 h, stable Basidiomycota

Cofactor

Cofactor Comment Organism Structure
FAD
-
 Basidiomycota