Cloned (Comment) | Organism |
---|---|
expressed in Escherichia coli BL21 Star DE3 cells | Trametes ochracea |
Protein Variants | Comment | Organism |
---|---|---|
F454A | the mutant shows about 40fold reduced catalytic efficiency compared to the wild type enzyme | Trametes ochracea |
F454Y | the mutant shows about 3.5fold reduced catalytic efficiency compared to the wild type enzyme | Trametes ochracea |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
H2O2 | oxidation of Phe454, which is located at the flexible active-site loop of the enzyme, is the first and main step in the inactivation of the enzyme by hydrogen peroxide | Trametes ochracea |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.712 | - |
D-glucose | mutant enzyme F454A, at pH 6.5 and 30°C | Trametes ochracea | |
0.851 | - |
D-glucose | wild type enzyme, at pH 6.5 and 30°C | Trametes ochracea | |
0.948 | - |
D-glucose | wild type enzyme F454Y, at pH 6.5 and 30°C | Trametes ochracea |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
270000 | - |
gel filtration | Trametes ochracea |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
D-glucose + O2 | Trametes ochracea | - |
2-dehydro-D-glucose + H2O2 | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Trametes ochracea | - |
- |
- |
Purification (Comment) | Organism |
---|---|
IMAC Ni-charged resin column chromatography | Trametes ochracea |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
D-glucose + O2 | - |
Trametes ochracea | 2-dehydro-D-glucose + H2O2 | - |
? |
Subunits | Comment | Organism |
---|---|---|
homotetramer | - |
Trametes ochracea |
Synonyms | Comment | Organism |
---|---|---|
POX | - |
Trametes ochracea |
pyranose:oxygen 2-oxidoreductase | - |
Trametes ochracea |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.479 | - |
D-glucose | mutant enzyme F454A, at pH 6.5 and 30°C | Trametes ochracea | |
16.3 | - |
D-glucose | wild type enzyme F454Y, at pH 6.5 and 30°C | Trametes ochracea | |
49 | - |
D-glucose | wild type enzyme, at pH 6.5 and 30°C | Trametes ochracea |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
FAD | - |
Trametes ochracea |
kcat/KM Value [1/mMs-1] | kcat/KM Value Maximum [1/mMs-1] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
1.47 | - |
D-glucose | mutant enzyme F454A, at pH 6.5 and 30°C | Trametes ochracea | |
17.2 | - |
D-glucose | wild type enzyme F454Y, at pH 6.5 and 30°C | Trametes ochracea | |
57.6 | - |
D-glucose | wild type enzyme, at pH 6.5 and 30°C | Trametes ochracea |