Cloned (Comment) | Organism |
---|---|
recombinant expression in Escherichia coli | Trametes ochracea |
Protein Variants | Comment | Organism |
---|---|---|
H167A | site-directed mutagenesis, the covalent linkag of the isoalloxazine ring of the cofactor FAD to His167 is destroyed, molecular dynamics, overview | Trametes ochracea |
H169S | site-directed mutagenesis, spectroscopic analysis and molecular dynamics of the T169S P2O-acetate complex | Trametes ochracea |
additional information | comparison the P2O active site in H167A, T169S or T169S-acetate complex with wild-type P2O-acetate complex, molecuar dynamics and spectroscopic analysi, detailed overview | Trametes ochracea |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
270000 | - |
- |
Trametes ochracea |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
D-glucose + O2 | Trametes ochracea | - |
2-dehydro-D-glucose + H2O2 | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Trametes ochracea | - |
- |
- |
Purification (Comment) | Organism |
---|---|
recmbinant enzyme from Escherichia coli by ammonium sulfate fractionation, anion exchange chromatography, and gel filtration | Trametes ochracea |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
D-glucose + O2 | - |
Trametes ochracea | 2-dehydro-D-glucose + H2O2 | - |
? |
Subunits | Comment | Organism |
---|---|---|
homotetramer | - |
Trametes ochracea |
Synonyms | Comment | Organism |
---|---|---|
P2O | - |
Trametes ochracea |
pyranose 2-Oxidase | - |
Trametes ochracea |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
7 | - |
assay at | Trametes ochracea |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
FAD | one FAD per subunit, Trp168 residue near the flavin isoalloxazine ring can potentially act as an electron donor to the excited flavin isoalloxazine ring, isoalloxazine is covalently linked to His167 | Trametes ochracea |
General Information | Comment | Organism |
---|---|---|
additional information | comparison the P2O active site in H167A, T169S or T169S-acetate complex with wild-type P2O-acetate complex, molecuar dynamics and spectroscopic analysi, detailed overview | Trametes ochracea |