Cloned (Comment) | Organism |
---|---|
mutant enzymes are expressed in Escherichia coli XL1-blue cells | Trametes ochracea |
Crystallization (Comment) | Organism |
---|---|
mutant enzymes H167A and T169, Ahanging drop vapor diffusion method, using 0.1 M sodium acetate (pH 4.6), 50 mM MgCl2, and 6% (w/v) monomethyl ether PEG 2000 | Trametes ochracea |
Protein Variants | Comment | Organism |
---|---|---|
H167A | FAD-deficient mutant | Trametes ochracea |
H548A | partially FAD-deficient mutant | Trametes ochracea |
H548N | partially FAD-deficient mutant | Trametes ochracea |
T169A | FAD-deficient mutant | Trametes ochracea |
T169G | the FAD content of the mutant enzyme is similar to the wild type enzyme | Trametes ochracea |
T169N | the FAD content of the mutant enzyme is similar to the wild type enzyme | Trametes ochracea |
T169S | the FAD content of the mutant enzyme is similar to the wild type enzyme | Trametes ochracea |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
acetate | competitive inhibitor | Trametes ochracea |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
D-glucose + O2 | Trametes ochracea | - |
2-dehydro-D-glucose + H2O2 | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Trametes ochracea | Q7ZA32 | synonym Trametes ochracea | - |
Purification (Comment) | Organism |
---|---|
- |
Trametes ochracea |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
D-glucose + O2 | - |
Trametes ochracea | 2-dehydro-D-glucose + H2O2 | - |
? |
Subunits | Comment | Organism |
---|---|---|
homotetramer | X-ray crystallograhy | Trametes ochracea |
Synonyms | Comment | Organism |
---|---|---|
P2O | - |
Trametes ochracea |
pyranose 2-Oxidase | - |
Trametes ochracea |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
FAD | - |
Trametes ochracea |