Literature summary for 1.1.3.10 extracted from

Salaheddin, C.; Spadiut, O.; Ludwig, R.; Tan, T.C.; Divne, C.; Haltrich, D.; Peterbauer, C.
Probing active-site residues of pyranose 2-oxidase from Trametes multicolor by semi-rational protein design (2009), Biotechnol. J., 4, 535-543.

Cloned(Commentary)

Cloned (Comment)	Organism
heterologously expressed in Escherichia coli BL21 (DE3)	Trametes ochracea

Protein Variants

Protein Variants	Comment	Organism
H548I	mutant, analysis of kinetic parameters	Trametes ochracea
H548R	mutant, analysis of kinetic parameters	Trametes ochracea
N593R	mutant, analysis of kinetic parameters	Trametes ochracea
Q448C	mutant, analysis of kinetic parameters	Trametes ochracea
Q448N	mutant, analysis of kinetic parameters	Trametes ochracea
Q448S	mutant, analysis of kinetic parameters	Trametes ochracea
R472G	mutant, analysis of kinetic parameters	Trametes ochracea
R472L	mutant, analysis of kinetic parameters	Trametes ochracea
T169G	mutant, analysis of kinetic parameters	Trametes ochracea
T169N	mutant, analysis of kinetic parameters	Trametes ochracea
T169S	mutant, analysis of kinetic parameters	Trametes ochracea
V546C	mutant, analysis of kinetic parameters	Trametes ochracea
V546C/T169N	mutant, analysis of kinetic parameters	Trametes ochracea
V546G	mutant, analysis of kinetic parameters	Trametes ochracea
V546G/T169G	mutant, analysis of kinetic parameters	Trametes ochracea
V546P	mutant, analysis of kinetic parameters	Trametes ochracea
V546P/T169G	mutant, analysis of kinetic parameters	Trametes ochracea

KM Value [mM]

KM Value [mM]	KM Value Maximum [mM]	Substrate	Comment	Organism
0.327	-	D-glucose	T169N, mutant, substrate D-glucose, 2,2'-azinobis(3-ethylbenzthiazoline-6-sulfonic acid)-peroxidase assay	Trametes ochracea
0.394	-	D-glucose	T169S, mutant, substrate D-glucose, 2,2'-azinobis(3-ethylbenzthiazoline-6-sulfonic acid)-peroxidase assay	Trametes ochracea
0.527	-	D-glucose	N593R, mutant, substrate D-glucose, 2,2'-azinobis(3-ethylbenzthiazoline-6-sulfonic acid)-peroxidase assay	Trametes ochracea
0.658	-	D-glucose	V546P/T169G, mutant, substrate D-glucose, 2,2'-azinobis(3-ethylbenzthiazoline-6-sulfonic acid)-peroxidase assay	Trametes ochracea
0.691	-	D-glucose	T169G, mutant, substrate D-glucose, 2,2'-azinobis(3-ethylbenzthiazoline-6-sulfonic acid)-peroxidase assay	Trametes ochracea
0.698	-	D-glucose	wild-type, substrate D-glucose, 2,2'-azinobis(3-ethylbenzthiazoline-6-sulfonic acid)-peroxidase assay	Trametes ochracea
0.773	-	D-glucose	R472G, mutant, substrate D-glucose, 2,2'-azinobis(3-ethylbenzthiazoline-6-sulfonic acid)-peroxidase assay	Trametes ochracea
0.8	11	D-glucose	H548I, mutant, substrate D-glucose, 2,2'-azinobis(3-ethylbenzthiazoline-6-sulfonic acid)-peroxidase assay	Trametes ochracea
0.88	-	D-glucose	H548R, mutant, substrate D-glucose, 2,2'-azinobis(3-ethylbenzthiazoline-6-sulfonic acid)-peroxidase assay	Trametes ochracea
0.886	-	D-glucose	R472L, mutant, substrate D-glucose, 2,2'-azinobis(3-ethylbenzthiazoline-6-sulfonic acid)-peroxidase assay	Trametes ochracea
0.952	-	D-glucose	V546C/T169N, mutant, substrate D-glucose, 2,2'-azinobis(3-ethylbenzthiazoline-6-sulfonic acid)-peroxidase assay	Trametes ochracea
0.983	-	D-galactose	V546P/T169G, mutant, substrate D-galactose, 2,2'-azinobis(3-ethylbenzthiazoline-6-sulfonic acid)-peroxidase assay	Trametes ochracea
0.984	-	D-glucose	V546G/T169G, mutant, substrate D-glucose, 2,2'-azinobis(3-ethylbenzthiazoline-6-sulfonic acid)-peroxidase assay	Trametes ochracea
2.2	-	D-glucose	V546P, mutant, substrate D-glucose, 2,2'-azinobis(3-ethylbenzthiazoline-6-sulfonic acid)-peroxidase assay	Trametes ochracea
2.48	-	D-galactose	T169G, mutant, substrate D-galactose, 2,2'-azinobis(3-ethylbenzthiazoline-6-sulfonic acid)-peroxidase assay	Trametes ochracea
3.06	-	D-glucose	V546C, mutant, substrate D-glucose, 2,2'-azinobis(3-ethylbenzthiazoline-6-sulfonic acid)-peroxidase assay	Trametes ochracea
3.56	-	D-galactose	T169N, mutant, substrate D-galactose, 2,2'-azinobis(3-ethylbenzthiazoline-6-sulfonic acid)-peroxidase assay	Trametes ochracea
5.7	-	D-glucose	V546G, mutant, substrate D-glucose, 2,2'-azinobis(3-ethylbenzthiazoline-6-sulfonic acid)-peroxidase assay	Trametes ochracea
6.38	-	D-galactose	R472G, mutant, substrate D-galactose, 2,2'-azinobis(3-ethylbenzthiazoline-6-sulfonic acid)-peroxidase assay	Trametes ochracea
7.21	-	D-galactose	R472L, mutant, substrate D-galactose, 2,2'-azinobis(3-ethylbenzthiazoline-6-sulfonic acid)-peroxidase assay	Trametes ochracea
7.39	-	D-galactose	N593R, mutant, substrate D-galactose, 2,2'-azinobis(3-ethylbenzthiazoline-6-sulfonic acid)-peroxidase assay	Trametes ochracea
8.09	-	D-galactose	wild-type, substrate D-galactose, 2,2'-azinobis(3-ethylbenzthiazoline-6-sulfonic acid)-peroxidase assay	Trametes ochracea
9.27	-	D-galactose	H548R, mutant, substrate D-galactose, 2,2'-azinobis(3-ethylbenzthiazoline-6-sulfonic acid)-peroxidase assay	Trametes ochracea
9.89	-	D-galactose	H548I, mutant, substrate D-galactose, 2,2'-azinobis(3-ethylbenzthiazoline-6-sulfonic acid)-peroxidase assay	Trametes ochracea
10.4	-	D-galactose	V546C/T169N, mutant, substrate D-galactose, 2,2'-azinobis(3-ethylbenzthiazoline-6-sulfonic acid)-peroxidase assay	Trametes ochracea
12.9	-	D-galactose	T169S, mutant, substrate D-galactose, 2,2'-azinobis(3-ethylbenzthiazoline-6-sulfonic acid)-peroxidase assay	Trametes ochracea
28	-	D-glucose	Q448N, mutant, substrate D-glucose, 2,2'-azinobis(3-ethylbenzthiazoline-6-sulfonic acid)-peroxidase assay	Trametes ochracea
29.4	-	D-galactose	V546G/T169G, mutant, substrate D-galactose, 2,2'-azinobis(3-ethylbenzthiazoline-6-sulfonic acid)-peroxidase assay	Trametes ochracea
30	-	D-glucose	Q448S, mutant, substrate D-glucose, 2,2'-azinobis(3-ethylbenzthiazoline-6-sulfonic acid)-peroxidase assay	Trametes ochracea
46.2	-	D-galactose	V546C, mutant, substrate D-galactose, 2,2'-azinobis(3-ethylbenzthiazoline-6-sulfonic acid)-peroxidase assay	Trametes ochracea
50.6	-	D-galactose	V546P, mutant, substrate D-galactose, 2,2'-azinobis(3-ethylbenzthiazoline-6-sulfonic acid)-peroxidase assay	Trametes ochracea
100	-	D-glucose	Q448C, mutant, substrate D-glucose, 2,2'-azinobis(3-ethylbenzthiazoline-6-sulfonic acid)-peroxidase assay	Trametes ochracea
200	-	D-galactose	V546G, mutant, substrate D-galactose, 2,2'-azinobis(3-ethylbenzthiazoline-6-sulfonic acid)-peroxidase assay	Trametes ochracea
750	-	D-galactose	Q448S, mutant, substrate D-galactose, 2,2'-azinobis(3-ethylbenzthiazoline-6-sulfonic acid)-peroxidase assay	Trametes ochracea
940	-	D-galactose	Q448N, mutant, substrate D-galactose, 2,2'-azinobis(3-ethylbenzthiazoline-6-sulfonic acid)-peroxidase assay	Trametes ochracea
1260	-	D-galactose	Q448C, mutant, substrate D-galactose, 2,2'-azinobis(3-ethylbenzthiazoline-6-sulfonic acid)-peroxidase assay	Trametes ochracea

Organism

Organism	UniProt	Comment	Textmining
Trametes ochracea	Q7ZA32	-	-

Purification (Commentary)

Purification (Comment)	Organism
Ni2+ immobilized column with Chelating Sepharose Fast Flow combined with Äkta Purifier System, concentrated in an Amicon Ultra Centrifugal Filter Device	Trametes ochracea

Substrates and Products (Substrate)

Substrates	Comment Substrates	Organism	Products	Comment (Products)	Rev.
D-galactose + 1,4-benzoquinone	-	Trametes ochracea	2-dehydro-D-galactose + 1,4-hydroquinone	-	?
D-galactose + 2,2'-azinobis(3-ethylbenzothiazoline-6-sulfonic acid) cation radical	-	Trametes ochracea	2-dehydro-D-galactose + 2,2'-azinobis(3-ethylbenzothiazoline-6-sulfonic acid)	-	?
D-galactose + O2	-	Trametes ochracea	2-dehydro-D-galactose + H2O2	-	?
D-glucose + 1,4-benzoquinone	-	Trametes ochracea	2-dehydro-D-glucose + 1,4-hydroquinone	-	?
D-glucose + 2,2'-azinobis(3-ethylbenzothiazoline-6-sulfonic acid) cation radical	-	Trametes ochracea	2-dehydro-D-glucose + 2,2'-azinobis(3-ethylbenzothiazoline-6-sulfonic acid)	-	?
D-glucose + O2	-	Trametes ochracea	2-dehydro-D-glucose + H2O2	-	?

Synonyms

Synonyms	Comment	Organism
P2Ox	-	Trametes ochracea
pyranose 2-Oxidase	-	Trametes ochracea
TmP2Ox	-	Trametes ochracea

Turnover Number [1/s]

Turnover Number Minimum [1/s]	Turnover Number Maximum [1/s]	Substrate	Comment	Organism
0.119	-	D-glucose	V546G/T169G, mutant, substrate D-glucose, 2,2'-azinobis(3-ethylbenzthiazoline-6-sulfonic acid)-peroxidase assay	Trametes ochracea
0.232	-	D-glucose	V546P/T169G, mutant, substrate D-glucose, 2,2'-azinobis(3-ethylbenzthiazoline-6-sulfonic acid)-peroxidase assay	Trametes ochracea
0.245	-	D-galactose	V546P/T169G, mutant, substrate D-galactose, 2,2'-azinobis(3-ethylbenzthiazoline-6-sulfonic acid)-peroxidase assay	Trametes ochracea
0.262	-	D-glucose	T169G, mutant, substrate D-glucose, 2,2'-azinobis(3-ethylbenzthiazoline-6-sulfonic acid)-peroxidase assay	Trametes ochracea
0.273	-	D-galactose	T169G, mutant, substrate D-galactose, 2,2'-azinobis(3-ethylbenzthiazoline-6-sulfonic acid)-peroxidase assay	Trametes ochracea
0.331	-	D-galactose	V546G/T169G, mutant, substrate D-galactose, 2,2'-azinobis(3-ethylbenzthiazoline-6-sulfonic acid)-peroxidase assay	Trametes ochracea
0.356	-	D-galactose	H548R, mutant, substrate D-galactose, 2,2'-azinobis(3-ethylbenzthiazoline-6-sulfonic acid)-peroxidase assay	Trametes ochracea
0.433	-	D-galactose	Q448N, mutant, substrate D-galactose, 2,2'-azinobis(3-ethylbenzthiazoline-6-sulfonic acid)-peroxidase assay	Trametes ochracea
0.622	-	D-galactose	Q448C, mutant, substrate D-galactose, 2,2'-azinobis(3-ethylbenzthiazoline-6-sulfonic acid)-peroxidase assay	Trametes ochracea
0.654	-	D-galactose	V546C/T169N, mutant, substrate D-galactose, 2,2'-azinobis(3-ethylbenzthiazoline-6-sulfonic acid)-peroxidase assay	Trametes ochracea
0.963	-	D-glucose	V546C/T169N, mutant, substrate D-glucose, 2,2'-azinobis(3-ethylbenzthiazoline-6-sulfonic acid)-peroxidase assay	Trametes ochracea
1.51	-	D-glucose	Q448C, mutant, substrate D-glucose, 2,2'-azinobis(3-ethylbenzthiazoline-6-sulfonic acid)-peroxidase assay	Trametes ochracea
1.53	-	D-galactose	Q448S, mutant, substrate D-galactose, 2,2'-azinobis(3-ethylbenzthiazoline-6-sulfonic acid)-peroxidase assay	Trametes ochracea
1.57	-	D-galactose	N593R, mutant, substrate D-galactose, 2,2'-azinobis(3-ethylbenzthiazoline-6-sulfonic acid)-peroxidase assay	Trametes ochracea
1.6	-	D-galactose	V546P, mutant, substrate D-galactose, 2,2'-azinobis(3-ethylbenzthiazoline-6-sulfonic acid)-peroxidase assay	Trametes ochracea
1.69	-	D-galactose	R472L, mutant, substrate D-galactose, 2,2'-azinobis(3-ethylbenzthiazoline-6-sulfonic acid)-peroxidase assay	Trametes ochracea
1.96	-	D-galactose	T169N, mutant, substrate D-galactose, 2,2'-azinobis(3-ethylbenzthiazoline-6-sulfonic acid)-peroxidase assay	Trametes ochracea
2.07	-	D-galactose	R472G, mutant, substrate D-galactose, 2,2'-azinobis(3-ethylbenzthiazoline-6-sulfonic acid)-peroxidase assay	Trametes ochracea
2.41	-	D-glucose	T169N, mutant, substrate D-glucose, 2,2'-azinobis(3-ethylbenzthiazoline-6-sulfonic acid)-peroxidase assay	Trametes ochracea
2.66	-	D-galactose	H548I, mutant, substrate D-galactose, 2,2'-azinobis(3-ethylbenzthiazoline-6-sulfonic acid)-peroxidase assay	Trametes ochracea
2.73	-	D-galactose	wild-type, substrate D-galactose, 2,2'-azinobis(3-ethylbenzthiazoline-6-sulfonic acid)-peroxidase assay	Trametes ochracea
4.51	-	D-galactose	V546G, mutant, substrate D-galactose, 2,2'-azinobis(3-ethylbenzthiazoline-6-sulfonic acid)-peroxidase assay	Trametes ochracea
4.73	-	D-glucose	Q448N, mutant, substrate D-glucose, 2,2'-azinobis(3-ethylbenzthiazoline-6-sulfonic acid)-peroxidase assay	Trametes ochracea
5.42	-	D-glucose	Q448S, mutant, substrate D-glucose, 2,2'-azinobis(3-ethylbenzthiazoline-6-sulfonic acid)-peroxidase assay	Trametes ochracea
5.53	-	D-galactose	T169S, mutant, substrate D-galactose, 2,2'-azinobis(3-ethylbenzthiazoline-6-sulfonic acid)-peroxidase assay	Trametes ochracea
6.57	-	D-galactose	V546C, mutant, substrate D-galactose, 2,2'-azinobis(3-ethylbenzthiazoline-6-sulfonic acid)-peroxidase assay	Trametes ochracea
9.2	-	D-glucose	H548R, mutant, substrate D-glucose, 2,2'-azinobis(3-ethylbenzthiazoline-6-sulfonic acid)-peroxidase assay	Trametes ochracea
15.6	-	D-glucose	N593R, mutant, substrate D-glucose, 2,2'-azinobis(3-ethylbenzthiazoline-6-sulfonic acid)-peroxidase assay	Trametes ochracea
15.8	-	D-glucose	V546P, mutant, substrate D-glucose, 2,2'-azinobis(3-ethylbenzthiazoline-6-sulfonic acid)-peroxidase assay	Trametes ochracea
21.8	-	D-glucose	T169S, mutant, substrate D-glucose, 2,2'-azinobis(3-ethylbenzthiazoline-6-sulfonic acid)-peroxidase assay	Trametes ochracea
23.6	-	D-glucose	V546G, mutant, substrate D-glucose, 2,2'-azinobis(3-ethylbenzthiazoline-6-sulfonic acid)-peroxidase assay	Trametes ochracea
30.9	-	D-glucose	R472L, mutant, substrate D-glucose, 2,2'-azinobis(3-ethylbenzthiazoline-6-sulfonic acid)-peroxidase assay	Trametes ochracea
31.6	-	D-glucose	H548I, mutant, substrate D-glucose, 2,2'-azinobis(3-ethylbenzthiazoline-6-sulfonic acid)-peroxidase assay	Trametes ochracea
32.2	-	D-glucose	R472G, mutant, substrate D-glucose, 2,2'-azinobis(3-ethylbenzthiazoline-6-sulfonic acid)-peroxidase assay	Trametes ochracea
35.4	-	D-glucose	wild-type, substrate D-glucose, 2,2'-azinobis(3-ethylbenzthiazoline-6-sulfonic acid)-peroxidase assay	Trametes ochracea
88.6	-	D-glucose	V546C, mutant, substrate D-glucose, 2,2'-azinobis(3-ethylbenzthiazoline-6-sulfonic acid)-peroxidase assay	Trametes ochracea