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Literature summary for 1.1.3.10 extracted from

  • Tasca, F.; Timur, S.; Ludwig, R.; Haltrich, D.; Volc, J.; Antiochia, R.; Gorton, L.
    Amperometric biosensors for detection of sugars based on the electrical wiring of different pyranose oxidases and pyranose dehydrogenases with osmium redox polymer on graphite electrodes (2007), Electroanalysis, 19, 294-302.
No PubMed abstract available
Search for more literature for 1.1.3.10

Application

Application Comment Organism
biotechnology studies on biosensors and biofuel cell anodes Trametes ochracea

Cloned(Commentary)

Cloned (Comment) Organism
expressed in Escherichia coli BL21-DE3, recombinant protein, plasmids pHL2e and pCL22 encoding the E542K mutant used Trametes ochracea

Protein Variants

Protein Variants Comment Organism
E542K inserted into plasmid pCL22 Trametes ochracea

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
additional information
-
 additional information D-mannose, glucosamine, beta-lactose, sucrose and D-maltose lack response signal, higher maltooligosaccharides good substrates for pyranose 2-oxidase-based biosensor Trametes ochracea
0.32
-
 D-glucose wild-type protein, 0.1 M ethanolamine buffer at pH 10.5 Trametes ochracea
0.35
-
 D-glucose recombinant protein with a hexa-histidine tag, 0.1 M ethanolamine buffer at pH 10.5 Trametes ochracea
0.71
-
 D-cellobiose 100 mM ethanolamine buffer, pH 10.5 Trametes ochracea
1.51
-
 D-glucose E542K with a hexa-histidine tag, 0.1 M ethanolamine buffer at pH 10.5 Trametes ochracea
1.55
-
 L-arabinose 100 mM ethanolamine buffer, pH 10.5 Trametes ochracea
2.72
-
 D-glucose recombinant protein, 0.1 M PBS buffer at pH 8 Trametes ochracea
6.02
-
 D-maltoheptaose 100 mM ethanolamine buffer, pH 10.5 Trametes ochracea
6.14
-
 D-maltopentaose 100 mM ethanolamine buffer, pH 10.5 Trametes ochracea
6.21
-
 D-xylose 100 mM ethanolamine buffer, pH 10.5 Trametes ochracea
6.25
-
 D-fucose 100 mM ethanolamine buffer, pH 10.5 Trametes ochracea
6.61
-
 D-maltotriose 100 mM ethanolamine buffer, pH 10.5 Trametes ochracea
7.51
-
 D-trehalose 100 mM ethanolamine buffer, pH 10.5 Trametes ochracea
10.32
-
 2-deoxy-D-galactose 100 mM ethanolamine buffer, pH 10.5 Trametes ochracea
11.81
-
 D-galactose 100 mM ethanolamine buffer, pH 10.5 Trametes ochracea
11.85
-
 2-deoxy-D-glucose 100 mM ethanolamine buffer, pH 10.5 Trametes ochracea

Organism

Organism UniProt Comment Textmining
Trametes ochracea Q7ZA32 wild-type, recombinant protein and commercial preparation
-

Purification (Commentary)

Purification (Comment) Organism
wild-type and recombinant protein, gel purification Trametes ochracea

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
additional information
-
 pyranose 2-oxidase-based biosensor system, electrical wiring of different types of pyranose oxidase with an osmium redox polymer on graphite electrodes, optimization studies using glucose as substrate, biosensors with best characteristics in terms of linear range, detection limit and sensitivity used for analyzing selectivity for different sugar substrates Trametes ochracea

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2-deoxy-D-galactose + O2 selectivity of pyranose 2-oxidase-based biosensor system for different sugar substrates analyzed Trametes ochracea ?
-
 ?
2-deoxy-D-glucose + O2 selectivity of pyranose 2-oxidase-based biosensor system for different sugar substrates analyzed Trametes ochracea ?
-
 ?
D-cellobiose + O2 selectivity of pyranose 2-oxidase-based biosensor system for different sugar substrates analyzed Trametes ochracea ?
-
 ?
D-fucose + O2 selectivity of pyranose 2-oxidase-based biosensor system for different sugar substrates analyzed Trametes ochracea ?
-
 ?
D-galactose + O2 selectivity of pyranose 2-oxidase-based biosensor system for different sugar substrates analyzed Trametes ochracea ?
-
 ?
D-glucose + O2 selectivity of pyranose 2-oxidase-based biosensor system for different sugar substrates analyzed Trametes ochracea 2-dehydro-D-glucose + H2O2
-
 ?
D-maltoheptaose + O2 selectivity of pyranose 2-oxidase-based biosensor system for different sugar substrates analyzed Trametes ochracea ?
-
 ?
D-maltopentaose + O2 selectivity of pyranose 2-oxidase-based biosensor system for different sugar substrates analyzed Trametes ochracea ?
-
 ?
D-maltotriose + O2 selectivity of pyranose 2-oxidase-based biosensor system for different sugar substrates analyzed Trametes ochracea ?
-
 ?
D-trehalose + O2 selectivity of pyranose 2-oxidase-based biosensor system for different sugar substrates analyzed Trametes ochracea ?
-
 ?
D-xylose + O2 selectivity of pyranose 2-oxidase-based biosensor system for different sugar substrates analyzed Trametes ochracea ?
-
 ?
L-arabinose + O2 selectivity of pyranose 2-oxidase-based biosensor system for different sugar substrates analyzed Trametes ochracea ?
-
 ?

Temperature Stability [°C]

Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
4
-
 under dry and wet storage conditions stable at 4°C, under dry storage conditions retainment of 100% of original activity after 2 days and of about 90% after one week, storage in working buffer solutions decreases activity by about 10% after one day and by more than 50% after one week Trametes ochracea

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
10.5
-
 activity assay at Trametes ochracea

pH Range

pH Minimum pH Maximum Comment Organism
8 11 optimal buffer combination for the pyranose 2-oxidase systems determined Trametes ochracea