KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.065 | - |
2,6-dichlorophenolindophenol | pH 6.5, substrate: D-glucose | Trametes ochracea | |
0.065 | - |
2,6-dichlorophenolindophenol | at pH 6.5 and 30°C | Trametes ochracea | |
0.07 | - |
2,2'-azinobis(3-ethylbenzthiazolinesulfonic acid) cation radical | pH 6.5, substrate: D-glucose | Trametes ochracea | |
0.088 | - |
tetrachloro-1,4-benzoquinone | pH 6.5, substrate: D-glucose | Trametes ochracea | |
0.09 | - |
oxygen | pH 6.5, substrate: D-glucose | Trametes ochracea | |
0.09 | - |
tetrabromo-1,4-benzoquinone | pH 6.5, substrate: D-glucose | Trametes ochracea | |
0.09 | - |
tetrabromo-1,4-benzoquinone | at pH 6.5 and 30°C | Trametes ochracea | |
0.094 | - |
2,6-dichlorophenolindophenol | pH 4.5, substrate: D-glucose | Trametes ochracea | |
0.094 | - |
2,6-dichlorophenolindophenol | at pH 4.5 and 30°C | Trametes ochracea | |
0.22 | - |
tetrafluoro-1,4-benzoquinone | pH 6.5, substrate: D-glucose | Trametes ochracea | |
0.3 | - |
1,4-benzoquinone | pH 4.5, substrate: D-glucose | Trametes ochracea | |
0.31 | - |
1,4-benzoquinone | pH 6.5, substrate: D-glucose | Trametes ochracea | |
0.35 | - |
methyl-1,4-benzoquinone | pH 4.5, substrate: D-glucose | Trametes ochracea | |
0.35 | - |
methyl-1,4-benzoquinone | at pH 4.5 and 30°C | Trametes ochracea | |
0.4 | - |
methyl-1,4-benzoquinone | pH 6.5, substrate: D-glucose | Trametes ochracea | |
0.4 | - |
methyl-1,4-benzoquinone | at pH 6.5 and 30°C | Trametes ochracea | |
0.74 | - |
D-glucose | - |
Trametes ochracea | |
0.83 | - |
2,6-dimethyl-1,4-benzoquinone | pH 4.5, substrate: D-glucose | Trametes ochracea | |
2.1 | - |
2,6-dimethyl-1,4-benzoquinone | pH 6.5, substrate: D-glucose | Trametes ochracea | |
3.9 | - |
5-thioglucose | - |
Trametes ochracea | |
9.2 | - |
D-galactose | - |
Trametes ochracea | |
30 | - |
D-xylose | - |
Trametes ochracea | |
36 | - |
D-allose | - |
Trametes ochracea | |
38 | - |
L-sorbose | - |
Trametes ochracea | |
38 | - |
D-gluconolactone | - |
Trametes ochracea | |
62 | - |
gentibiose | - |
Trametes ochracea | |
97 | - |
L-arabinose | - |
Trametes ochracea | |
110 | - |
D-mannoheptose | - |
Trametes ochracea |
Localization | Comment | Organism | GeneOntology No. | Textmining |
---|
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
65000 | - |
4 * 65000, SDS-PAGE, corroborated by electron microscopy studies | Trametes ochracea |
262000 | - |
gel filtration | Trametes ochracea |
270000 | - |
equilibrium ultracentrifugation | Trametes ochracea |
278000 | - |
electron microscopy | Trametes ochracea |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Trametes ochracea | - |
- |
- |
Purification (Comment) | Organism |
---|---|
using ammonium sulfate fractionation, hydrophobic interaction, ion-exchange chromatography, and gel filtration | Trametes ochracea |
Source Tissue | Comment | Organism | Textmining |
---|
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
12 | - |
- |
Trametes ochracea |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
1,5-anhydro-D-sorbitol + O2 | - |
Trametes ochracea | ? | - |
? | |
1,6-anhydro-D-glucose + O2 | - |
Trametes ochracea | ? | - |
? | |
2,6-dimethyl-1,4-benzoquinone + O2 | - |
Trametes ochracea | ? + H2O2 | - |
? | |
3-deoxy-D-glucose + O2 | - |
Trametes ochracea | 2-keto-3-deoxy-D-glucose + H2O2 | - |
? | |
5-thioglucose + O2 | 24% relative activity to D-glucose | Trametes ochracea | 2-keto-5-thioglucose + H2O2 | - |
? | |
6-deoxy-D-glucose + O2 | - |
Trametes ochracea | 2-keto-6-deoxy-D-glucose + H2O2 | - |
? | |
allose + O2 | 38% relative activity to D-glucose | Trametes ochracea | ? | - |
? | |
D-fucose + O2 | - |
Trametes ochracea | ? | - |
? | |
D-galactose + O2 | 5.7% relative activity to D-glucose | Trametes ochracea | 2-dehydro-D-galactose + H2O2 | - |
? | |
D-gluconolactone + O2 | 64% relative activity to D-glucose | Trametes ochracea | ? + H2O2 | - |
? | |
D-glucose + 2,6-dichlorophenolindophenol | - |
Trametes ochracea | 2-dehydro-D-glucose + reduced 2,6-dichlorophenolindophenol | - |
r | |
D-glucose + methyl-1,4-benzoquinone | - |
Trametes ochracea | 2-dehydro-D-glucose + methylhydroquinone | - |
r | |
D-glucose + O2 | 100% relative activity | Trametes ochracea | D-glucosone + H2O2 | - |
? | |
D-glucose + O2 | D-glucose is the preferred substrate | Trametes ochracea | D-glucosone + H2O2 | - |
? | |
D-glucose + tetrabromo-1,4-benzoquinone | - |
Trametes ochracea | 2-dehydro-D-glucose + tetrabromohydroquinone | - |
r | |
D-mannoheptose + O2 | 8.2% relative activity to D-glucose | Trametes ochracea | ? | - |
? | |
D-xylose + O2 | 56% relative activity to D-glucose | Trametes ochracea | D-xylosone + H2O2 | - |
? | |
gentibiose + O2 | 23% relative activity to D-glucose | Trametes ochracea | 2-keto-D-gentibiose + H2O2 | - |
? | |
L-arabinose + O2 | 1.5% relative activity to D-glucose | Trametes ochracea | ? | - |
? | |
L-idose + O2 | - |
Trametes ochracea | ? | - |
? | |
L-sorbose + O2 | 99% relative activity to D-glucose | Trametes ochracea | 5-dehydro-D-fructose + H2O2 | - |
? | |
tetrafluoro-1,4-benzoquinone + O2 | - |
Trametes ochracea | ? + H2O2 | - |
? |
Subunits | Comment | Organism |
---|---|---|
tetramer | 4 * 65000, SDS-PAGE, corroborated by electron microscopy studies | Trametes ochracea |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
4 | - |
electron acceptor: 2,6-dimethyl-1,4-benzoquinone | Trametes ochracea |
4.5 | - |
electron acceptor: 2,6-dichloroindophenol | Trametes ochracea |
5.5 | 6.5 | electron acceptor: oxygen or 1,4-benzoquinone | Trametes ochracea |
6 | 8 | electron acceptor: tetrachloro-1,4-benzoquinone | Trametes ochracea |
pH Stability | pH Stability Maximum | Comment | Organism |
---|---|---|---|
3 | - |
half-life: 13 days | Trametes ochracea |
3.5 | - |
half-life: 52 days | Trametes ochracea |
4 | - |
half-life: 130 days | Trametes ochracea |
4.5 | - |
half-life: 350 days | Trametes ochracea |
5 | 7 | extremely stable, half-life more than 500 days | Trametes ochracea |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
FAD | covalently bound | Trametes ochracea |