Protein Variants | Comment | Organism |
---|---|---|
additional information | generation of a single deletion mutant strain of the gene coding for PedE resulting in strain GN104, and of a double mutant with deletion of both genes PedE and PedH, strain GN127 | Pseudomonas putida |
additional information | generation of a single deletion mutant strain of the gene coding for PedH resulting in strain GN116, and of a double mutant with deletion of both genes PedE and PedH, strain GN127 | Pseudomonas putida |
Localization | Comment | Organism | GeneOntology No. | Textmining |
---|---|---|---|---|
periplasm | - |
Pseudomonas putida | - |
- |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
butanol + 2 cytochrome c | Pseudomonas putida | - |
butyraldehyde + 2 reduced cytochrome c | - |
r | |
butanol + 2 cytochrome c | Pseudomonas putida KT 2240 | - |
butyraldehyde + 2 reduced cytochrome c | - |
r | |
ethanol + 2 cytochrome c | Pseudomonas putida | - |
acetaldehyde + 2 reduced cytochrome c | - |
r | |
ethanol + 2 cytochrome c | Pseudomonas putida KT 2240 | - |
acetaldehyde + 2 reduced cytochrome c | - |
r |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Pseudomonas putida | B1N7J0 | - |
- |
Pseudomonas putida | B1N7J5 | - |
- |
Pseudomonas putida KT 2240 | B1N7J0 | - |
- |
Pseudomonas putida KT 2240 | B1N7J5 | - |
- |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
butanol + 2 cytochrome c | - |
Pseudomonas putida | butyraldehyde + 2 reduced cytochrome c | - |
r | |
butanol + 2 cytochrome c | - |
Pseudomonas putida KT 2240 | butyraldehyde + 2 reduced cytochrome c | - |
r | |
ethanol + 2 cytochrome c | - |
Pseudomonas putida | acetaldehyde + 2 reduced cytochrome c | - |
r | |
ethanol + 2 cytochrome c | - |
Pseudomonas putida KT 2240 | acetaldehyde + 2 reduced cytochrome c | - |
r |
Synonyms | Comment | Organism |
---|---|---|
ethanol dehydrogenase | - |
Pseudomonas putida |
PedE | - |
Pseudomonas putida |
PedH | - |
Pseudomonas putida |
PP_2674 | - |
Pseudomonas putida |
PP_2679 | - |
Pseudomonas putida |
PQQ-dependent alcohol dehydrogenase | - |
Pseudomonas putida |
quinoprotein alcohol dehydrogenase | - |
Pseudomonas putida |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
cytochrome c | - |
Pseudomonas putida | |
pyrroloquinoline quinone | PQQ, a prosthetic group | Pseudomonas putida |
General Information | Comment | Organism |
---|---|---|
malfunction | single deletions of genes coding for PQQ-dependent alcohol dehydrogenases PedE and PedH have only minor effects on growth rates, indicating that Pseudomonas putida strain KT2440 can use both enzymes in a redundant fashion for the metabolization of butanol. Growth of mutants lacking PedE and PedH on n-butanol is significantly impaired, but not completely inhibited, suggesting that additional alcohol dehydrogenases can at least partially complement their function in strain KT2440 | Pseudomonas putida |
metabolism | butanol is oxidized to butyraldehyde by PedE and PedH and then further oxidized to butyric acid by the aldehyde dehydrogenase PedI. Both enzymes, PedE and PedH, are directly involved in butanol oxidation in Pseudomonas putida KT2440 | Pseudomonas putida |