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Literature summary for 1.1.2.8 extracted from

  • Simon, O.; Klebensberger, J.; Muekschel, B.; Klaiber, I.; Graf, N.; Altenbuchner, J.; Huber, A.; Hauer, B.; Pfannstiel, J.
    Analysis of the molecular response of Pseudomonas putida KT2440 to the next-generation biofuel n-butanol (2015), J. Proteomics, 122, 11-25 .
    View publication on PubMed

Protein Variants

Protein Variants Comment Organism
additional information generation of a single deletion mutant strain of the gene coding for PedE resulting in strain GN104, and of a double mutant with deletion of both genes PedE and PedH, strain GN127 Pseudomonas putida
additional information generation of a single deletion mutant strain of the gene coding for PedH resulting in strain GN116, and of a double mutant with deletion of both genes PedE and PedH, strain GN127 Pseudomonas putida

Localization

Localization Comment Organism GeneOntology No. Textmining
periplasm
-
Pseudomonas putida
-
-

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
butanol + 2 cytochrome c Pseudomonas putida
-
butyraldehyde + 2 reduced cytochrome c
-
r
butanol + 2 cytochrome c Pseudomonas putida KT 2240
-
butyraldehyde + 2 reduced cytochrome c
-
r
ethanol + 2 cytochrome c Pseudomonas putida
-
acetaldehyde + 2 reduced cytochrome c
-
r
ethanol + 2 cytochrome c Pseudomonas putida KT 2240
-
acetaldehyde + 2 reduced cytochrome c
-
r

Organism

Organism UniProt Comment Textmining
Pseudomonas putida B1N7J0
-
-
Pseudomonas putida B1N7J5
-
-
Pseudomonas putida KT 2240 B1N7J0
-
-
Pseudomonas putida KT 2240 B1N7J5
-
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
butanol + 2 cytochrome c
-
Pseudomonas putida butyraldehyde + 2 reduced cytochrome c
-
r
butanol + 2 cytochrome c
-
Pseudomonas putida KT 2240 butyraldehyde + 2 reduced cytochrome c
-
r
ethanol + 2 cytochrome c
-
Pseudomonas putida acetaldehyde + 2 reduced cytochrome c
-
r
ethanol + 2 cytochrome c
-
Pseudomonas putida KT 2240 acetaldehyde + 2 reduced cytochrome c
-
r

Synonyms

Synonyms Comment Organism
ethanol dehydrogenase
-
Pseudomonas putida
PedE
-
Pseudomonas putida
PedH
-
Pseudomonas putida
PP_2674
-
Pseudomonas putida
PP_2679
-
Pseudomonas putida
PQQ-dependent alcohol dehydrogenase
-
Pseudomonas putida
quinoprotein alcohol dehydrogenase
-
Pseudomonas putida

Cofactor

Cofactor Comment Organism Structure
cytochrome c
-
Pseudomonas putida
pyrroloquinoline quinone PQQ, a prosthetic group Pseudomonas putida

General Information

General Information Comment Organism
malfunction single deletions of genes coding for PQQ-dependent alcohol dehydrogenases PedE and PedH have only minor effects on growth rates, indicating that Pseudomonas putida strain KT2440 can use both enzymes in a redundant fashion for the metabolization of butanol. Growth of mutants lacking PedE and PedH on n-butanol is significantly impaired, but not completely inhibited, suggesting that additional alcohol dehydrogenases can at least partially complement their function in strain KT2440 Pseudomonas putida
metabolism butanol is oxidized to butyraldehyde by PedE and PedH and then further oxidized to butyric acid by the aldehyde dehydrogenase PedI. Both enzymes, PedE and PedH, are directly involved in butanol oxidation in Pseudomonas putida KT2440 Pseudomonas putida