Localization | Comment | Organism | GeneOntology No. | Textmining |
---|---|---|---|---|
membrane | bound | Gluconacetobacter diazotrophicus | 16020 | - |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Iron | one [2Fe-2S] cluster per enzyme | Gluconacetobacter diazotrophicus |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
45000 | - |
1 * 72000 + 1 * 45000, inactive enzyme form, SDS-PAGE | Gluconacetobacter diazotrophicus |
45000 | - |
2 * 72000 + 2 * 45000, dimer of dimers, active enzyme form, SDS-PAGE | Gluconacetobacter diazotrophicus |
72000 | - |
1 * 72000 + 1 * 45000, inactive enzyme form, SDS-PAGE | Gluconacetobacter diazotrophicus |
72000 | - |
2 * 72000 + 2 * 45000, dimer of dimers, active enzyme form, SDS-PAGE | Gluconacetobacter diazotrophicus |
115000 | - |
inactive enzyme form, native PAGE | Gluconacetobacter diazotrophicus |
120000 | - |
inactive enzyme form, gel filtration | Gluconacetobacter diazotrophicus |
330000 | - |
active enzyme form, gel filtration | Gluconacetobacter diazotrophicus |
345000 | - |
active enzyme form, native PAGE | Gluconacetobacter diazotrophicus |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Gluconacetobacter diazotrophicus | - |
- |
- |
Gluconacetobacter diazotrophicus PAL5 | - |
- |
- |
Purification (Comment) | Organism |
---|---|
native enzyme from membranes by two steps of anion exchange chormatography, hydroxyapatite chromatography, and gel filtration | Gluconacetobacter diazotrophicus |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
additional information | ADH is largely expressed in its active form | Gluconacetobacter diazotrophicus | - |
Subunits | Comment | Organism |
---|---|---|
heterodimer | 1 * 72000 + 1 * 45000, inactive enzyme form, SDS-PAGE | Gluconacetobacter diazotrophicus |
heterotetramer | 2 * 72000 + 2 * 45000, dimer of dimers, active enzyme form, SDS-PAGE | Gluconacetobacter diazotrophicus |
Synonyms | Comment | Organism |
---|---|---|
PQQ-alcohol dehydrogenase | - |
Gluconacetobacter diazotrophicus |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
cytochrome c | prosthetic groups, four per enzyme | Gluconacetobacter diazotrophicus | |
additional information | one [2Fe-2S] cluster per enzyme | Gluconacetobacter diazotrophicus | |
pyrroloquinoline quinone | prosthetic group, one per enzyme | Gluconacetobacter diazotrophicus |
General Information | Comment | Organism |
---|---|---|
additional information | the enzyme occurs in active and inactive forms, overview. Active ADHa is brought back by ethanol to its full reduction state, but in inactive ADHi, only one-quarter of the total heme c is reduced, pH dependencies and redox potentials of cofactors, overview | Gluconacetobacter diazotrophicus |