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Literature summary for 1.1.2.8 extracted from

  • Gomez-Manzo, S.; Gonzalez-Valdez, A.A.; Oria-Hernandez, J.; Reyes-Vivas, H.; Arreguin-Espinosa, R.; Kroneck, P.M.; Sosa-Torres, M.E.; Escamilla, J.E.
    The active (ADHa) and inactive (ADHi) forms of the PQQ-alcohol dehydrogenase from Gluconacetobacter diazotrophicus differ in their respective oligomeric structures and redox state of their corresponding prosthetic groups (2012), FEMS Microbiol. Lett., 328, 106-113.
    View publication on PubMed

Localization

Localization Comment Organism GeneOntology No. Textmining
membrane bound Gluconacetobacter diazotrophicus 16020
-

Metals/Ions

Metals/Ions Comment Organism Structure
Iron one [2Fe-2S] cluster per enzyme Gluconacetobacter diazotrophicus

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
45000
-
1 * 72000 + 1 * 45000, inactive enzyme form, SDS-PAGE Gluconacetobacter diazotrophicus
45000
-
2 * 72000 + 2 * 45000, dimer of dimers, active enzyme form, SDS-PAGE Gluconacetobacter diazotrophicus
72000
-
1 * 72000 + 1 * 45000, inactive enzyme form, SDS-PAGE Gluconacetobacter diazotrophicus
72000
-
2 * 72000 + 2 * 45000, dimer of dimers, active enzyme form, SDS-PAGE Gluconacetobacter diazotrophicus
115000
-
inactive enzyme form, native PAGE Gluconacetobacter diazotrophicus
120000
-
inactive enzyme form, gel filtration Gluconacetobacter diazotrophicus
330000
-
active enzyme form, gel filtration Gluconacetobacter diazotrophicus
345000
-
active enzyme form, native PAGE Gluconacetobacter diazotrophicus

Organism

Organism UniProt Comment Textmining
Gluconacetobacter diazotrophicus
-
-
-
Gluconacetobacter diazotrophicus PAL5
-
-
-

Purification (Commentary)

Purification (Comment) Organism
native enzyme from membranes by two steps of anion exchange chormatography, hydroxyapatite chromatography, and gel filtration Gluconacetobacter diazotrophicus

Source Tissue

Source Tissue Comment Organism Textmining
additional information ADH is largely expressed in its active form Gluconacetobacter diazotrophicus
-

Subunits

Subunits Comment Organism
heterodimer 1 * 72000 + 1 * 45000, inactive enzyme form, SDS-PAGE Gluconacetobacter diazotrophicus
heterotetramer 2 * 72000 + 2 * 45000, dimer of dimers, active enzyme form, SDS-PAGE Gluconacetobacter diazotrophicus

Synonyms

Synonyms Comment Organism
PQQ-alcohol dehydrogenase
-
Gluconacetobacter diazotrophicus

Cofactor

Cofactor Comment Organism Structure
cytochrome c prosthetic groups, four per enzyme Gluconacetobacter diazotrophicus
additional information one [2Fe-2S] cluster per enzyme Gluconacetobacter diazotrophicus
pyrroloquinoline quinone prosthetic group, one per enzyme Gluconacetobacter diazotrophicus

General Information

General Information Comment Organism
additional information the enzyme occurs in active and inactive forms, overview. Active ADHa is brought back by ethanol to its full reduction state, but in inactive ADHi, only one-quarter of the total heme c is reduced, pH dependencies and redox potentials of cofactors, overview Gluconacetobacter diazotrophicus