Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Ca2+ | stimulates, the enzyme purified in presence of EDTA shows no activity until pyrroloquinoline quinone, Ca2+, Mg2+ are added | Pseudomonas sp. | |
Mg2+ | stimulating effect is far less than with Ca2+, the enzyme purified in presence of EDTA shows no activity until pyrroloquinoline quinone, Ca2+, Mg2+ are added | Pseudomonas sp. |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
67000 | - |
gel filtration | Pseudomonas sp. |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Pseudomonas sp. | - |
- |
- |
Pseudomonas sp. 113P3 | - |
- |
- |
Purification (Comment) | Organism |
---|---|
- |
Pseudomonas sp. |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
19.8 | - |
- |
Pseudomonas sp. |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
1,3-butanediol + oxidized 2,6-dichlorophenolindophenol | 57% of activity with 2,4-pentanediol | Pseudomonas sp. | ? | - |
? | |
1,3-butanediol + oxidized 2,6-dichlorophenolindophenol | 57% of activity with 2,4-pentanediol | Pseudomonas sp. 113P3 | ? | - |
? | |
1,3-cyclohexanediol + oxidized 2,6-dichlorophenolindophenol | 393% of activity with 2,4-pentanediol | Pseudomonas sp. | ? | - |
? | |
1,3-cyclohexanediol + oxidized 2,6-dichlorophenolindophenol | 393% of activity with 2,4-pentanediol | Pseudomonas sp. 113P3 | ? | - |
? | |
2,4-pentanediol + oxidized 2,6-dichlorophenolindophenol | enzyme shows only activity against meso-2,4-pentanediol | Pseudomonas sp. | ? | - |
? | |
2,4-pentanediol + oxidized 2,6-dichlorophenolindophenol | enzyme shows only activity against meso-2,4-pentanediol | Pseudomonas sp. 113P3 | ? | - |
? | |
additional information | no activity with methanol, ethanol, 1-propanol, 1-butanol, 4-heptanol, 2-hexanol and 2-pentanol | Pseudomonas sp. | ? | - |
? | |
additional information | no activity with methanol, ethanol, 1-propanol, 1-butanol, 4-heptanol, 2-hexanol and 2-pentanol | Pseudomonas sp. 113P3 | ? | - |
? | |
polyvinyl alcohol + oxidized 2,6-dichlorophenolindophenol | activity is 6.7fold higher than activity with 2,4-pentanediol | Pseudomonas sp. | polyvinyl alcohol containing oxo-groups + reduced 2,6-dichlorophenolindophenol | - |
? | |
polyvinyl alcohol + oxidized 2,6-dichlorophenolindophenol | activity is 6.7fold higher than activity with 2,4-pentanediol | Pseudomonas sp. 113P3 | polyvinyl alcohol containing oxo-groups + reduced 2,6-dichlorophenolindophenol | - |
? |
Subunits | Comment | Organism |
---|---|---|
monomer | 1 * 67000, SDS-PAGE | Pseudomonas sp. |
Synonyms | Comment | Organism |
---|---|---|
PVA dehydrogenase | - |
Pseudomonas sp. |
PVADH | - |
Pseudomonas sp. |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
37 | - |
- |
Pseudomonas sp. |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
7.2 | - |
- |
Pseudomonas sp. |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
heme | hemequino-protein, 1.03 mol of heme per mol of apoenzyme | Pseudomonas sp. | |
pyrroloquinoline quinone | the enzyme purified in presence of EDTA shows no activity until pyrroloquinoline quinone, Ca2+, Mg2+ are added | Pseudomonas sp. |