Inhibitors | Comment | Organism | Structure |
---|---|---|---|
oxalate | - |
Homo sapiens |
Localization | Comment | Organism | GeneOntology No. | Textmining |
---|---|---|---|---|
mitochondrial inner membrane | the enzyme is located on the inner face of the inner mitochondrial membrane | Homo sapiens | 5743 | - |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
(R)-lactate + 2 ferricytochrome c | Homo sapiens | - |
pyruvate + 2 ferrocytochrome c | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Homo sapiens | Q86WU2 | - |
- |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
PC-3 cell | - |
Homo sapiens | - |
PNT-1A cell | - |
Homo sapiens | - |
prostate cancer cell | - |
Homo sapiens | - |
prostate gland | - |
Homo sapiens | - |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
(R)-lactate + 2 ferricytochrome c | - |
Homo sapiens | pyruvate + 2 ferrocytochrome c | - |
? | |
additional information | D-LDH activity assay and (R)-lactate-dependent cyt c and flavin reduction | Homo sapiens | ? | - |
? |
Synonyms | Comment | Organism |
---|---|---|
D-LDH | - |
Homo sapiens |
DLD | - |
Homo sapiens |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
37 | - |
assay at | Homo sapiens |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
7.5 | - |
assay at | Homo sapiens |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
FAD | the enzyme is a flavoprotein. The flavin/pyridine cofactor redox state is detected fluorimetrically in cell homogenates | Homo sapiens | |
ferricytochrome c | - |
Homo sapiens |
General Information | Comment | Organism |
---|---|---|
metabolism | prostate cancer cells metabolize D-lactate inside mitochondria via a D-lactate dehydrogenase which is more active and highly expressed than in normal cells. D-Lactate can enter prostate mitochondria and cause the export of newly synthesized malate in a carrier-mediated manner, with the rate of malate efflux from mitochondria being twofold higher in cancer compared to healthy cells, overview | Homo sapiens |
physiological function | the mitochondrial D-lactate dehydrogenase, a membrane flavoprotein, from both cancer (PC-3) and normal (PNT1A) prostate cells can metabolize D-lactate in an energy competent manner | Homo sapiens |