Any feedback?
Literature summary for 1.1.2.4 extracted from
- D-lactate dehydrogenase of Peptostreptococcus elsdenii (1975), Methods Enzymol., 41B, 309-312.
Inhibitors
| Inhibitors | Comment | Organism | Structure |
|---|---|---|---|
| o-phenanthroline | very strong, reactivation with Zn2+ and Co2+ | Megasphaera elsdenii |  |
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.013 | - |
D-lactate | with ferricyanide | Megasphaera elsdenii | |
| 0.23 | - |
ferricyanide | - |
Megasphaera elsdenii | |
| 26 | - |
D-lactate | with complete electron transport system | Megasphaera elsdenii | |
Metals/Ions
| Metals/Ions | Comment | Organism | Structure |
|---|---|---|---|
| Co2+ | restores activity after inhibition with o-phenanthroline | Megasphaera elsdenii | |
| ferricyanide | can substitute for cytochrome c | Megasphaera elsdenii | |
| Zn2+ | restores activity after inhibition with o-phenanthroline | Megasphaera elsdenii | |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 125000 | - |
- |
Megasphaera elsdenii |
Natural Substrates/ Products (Substrates)
| Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| (R)-lactate + 2 ferricytochrome c | Megasphaera elsdenii | - |
pyruvate + ferrocytochrome c | - |
? | |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Megasphaera elsdenii | - |
- |
- |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
- |
Megasphaera elsdenii |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| (R)-lactate + 2 ferricytochrome c | - |
Megasphaera elsdenii | pyruvate + ferrocytochrome c | - |
? | |
| (R)-lactate + ferricytochrome c | - |
Megasphaera elsdenii | pyruvate + ferrocytochrome c | - |
? | |
| D-2-hydroxybutyrate + cytochrome c | - |
Megasphaera elsdenii | 2-oxobutanoate + ferrocytochrome c | - |
? | |
| D-lactate + ferricyanide | - |
Megasphaera elsdenii | pyruvate + ferrocyanide + H+ | - |
r | |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 6 | 8 | - |
Megasphaera elsdenii |
Cofactor
| Cofactor | Comment | Organism | Structure |
|---|---|---|---|
| cytochrome c | - |
Megasphaera elsdenii | |
| FAD | - |
Megasphaera elsdenii | |
| additional information | no activity with NAD+ or NADP+ | Megasphaera elsdenii |
Use of this online version of BRENDA is free under the CC BY 4.0 license. See terms of use for full details.
Release 2023.1 (January 2023)
Legal
Curated at
Member of
