Protein Variants | Comment | Organism |
---|---|---|
H373Q | the mutation results in a 34 orders of magnitude decrease in kcat and a slight increase in L-lactate Km | Saccharomyces cerevisiae |
L230A | the mutant flavocytochrome b2 displays increased selectivity for (S)-2-hydroxyoctanoate over L-lactate by a factor of 40 (kcat/Km) | Saccharomyces cerevisiae |
L230A/A198G | the double mutant enzyme has a 6fold greater catalytic efficiency with L-mandelate than with L-lactate | Saccharomyces cerevisiae |
Y254F | the mutant enzyme has a Vmax value some 28fold lower than that of the wild type enzyme and a slightly raised L-lactate Km value | Saccharomyces cerevisiae |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
57500 | - |
4 * 57500, X-ray crystallography | Saccharomyces cerevisiae |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
L-lactate + ferricytochrome c | Saccharomyces cerevisiae | - |
pyruvate + ferrocytochrome c + H+ | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Saccharomyces cerevisiae | P00175 | - |
- |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
L-lactate + ferricytochrome c | - |
Saccharomyces cerevisiae | pyruvate + ferrocytochrome c + H+ | - |
? |
Subunits | Comment | Organism |
---|---|---|
homotetramer | 4 * 57500, X-ray crystallography | Saccharomyces cerevisiae |
Synonyms | Comment | Organism |
---|---|---|
flavocytochrome b2 | - |
Saccharomyces cerevisiae |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
cytochrome c | - |
Saccharomyces cerevisiae | |
heme | - |
Saccharomyces cerevisiae |
Organism | Comment | Expression |
---|---|---|
Saccharomyces cerevisiae | flavocytochrome b2 production is induced by the presence of oxygen and L-lactate | up |
General Information | Comment | Organism |
---|---|---|
metabolism | the pyruvate produced as a result of L-lactate oxidation is utilised by the Krebs cycle, but flavocytochrome b2 also forms part of a short respiratory electron transport chain which results in one ATP molecule being produced for every L-lactate consumed | Saccharomyces cerevisiae |