Cloned (Comment) | Organism |
---|---|
in Escherichia coli MM294 | Saccharomyces cerevisiae |
Crystallization (Comment) | Organism |
---|---|
crystallization of Y143F mutant, vapor diffusion technique in presence of PEG 4000 | Saccharomyces cerevisiae |
Protein Variants | Comment | Organism |
---|---|---|
H373Q | mutant | Saccharomyces cerevisiae |
Y143F | turnover reduced to 15% | Saccharomyces cerevisiae |
Y254L | only traces of activity | Saccharomyces cerevisiae |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
Fluoropyruvate | - |
Saccharomyces cerevisiae | |
pyruvate | competitive at low concentrations, non-competitve at high concentrations | Saccharomyces cerevisiae |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.0001 | - |
L-lactate | Y254L mutant enzyme with 2,6-dichloroindophenol as electron acceptor | Saccharomyces cerevisiae | |
0.037 | - |
L-lactate | wild type enzyme with 2,6-dichloroindophenol as electron acceptor | Saccharomyces cerevisiae | |
0.04 | - |
L-lactate | Y143F mutant enzyme with 2,6-dichloroindophenol as electron acceptor | Saccharomyces cerevisiae | |
0.13 | - |
L-lactate | H373Q mutant enzyme with 2,6-dichloroindophenol as electron acceptor | Saccharomyces cerevisiae |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Fe2+ | bound to enzyme | Saccharomyces cerevisiae |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
(S)-lactate + 2 ferricytochrome c | Saccharomyces cerevisiae | can feed electrons to respiratory chain at the level of cytochrome c | pyruvate + 2 ferrocytochrome c + 2 H+ | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Saccharomyces cerevisiae | - |
- |
- |
Purification (Comment) | Organism |
---|---|
wild type and mutant enzymes | Saccharomyces cerevisiae |
Storage Stability | Organism |
---|---|
-80°C as saturated solution | Saccharomyces cerevisiae |
4°C, precipitate from 70% saturated ammonium sulfate, under nitrogen, stable for several weeks | Saccharomyces cerevisiae |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
(S)-lactate + 2 ferricytochrome c | - |
Saccharomyces cerevisiae | pyruvate + 2 ferrocytochrome c + 2 H+ | - |
? | |
(S)-lactate + 2 ferricytochrome c | can feed electrons to respiratory chain at the level of cytochrome c | Saccharomyces cerevisiae | pyruvate + 2 ferrocytochrome c + 2 H+ | - |
? | |
additional information | 2,6-dichlorophenolindophenol used as electron acceptor, 10times more sensitive than ferricyanide | Saccharomyces cerevisiae | ? | - |
? | |
additional information | electron acceptors other than ferricytochrome c used | Saccharomyces cerevisiae | ? | - |
? |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.39 | - |
L-lactate | Y254L mutant enzyme | Saccharomyces cerevisiae | |
60 | - |
L-lactate | Y143F mutant enzyme | Saccharomyces cerevisiae |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
additional information | electron acceptors other than ferricytochrome c used | Saccharomyces cerevisiae | |
additional information | 2,6-dichlorophenolindophenol used as electron acceptor, 10-fold more sensitive than ferricyanide | Saccharomyces cerevisiae |
Ki Value [mM] | Ki Value maximum [mM] | Inhibitor | Comment | Organism | Structure |
---|---|---|---|---|---|
3 | - |
pyruvate | competitive | Saccharomyces cerevisiae | |
30 | - |
pyruvate | non-competitive | Saccharomyces cerevisiae |