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Literature summary for 1.1.2.3 extracted from

  • Gaume, B.; Sharp, R.E.; Manson, F.D.C.; Chapman, S.K.; Reid, G.A.; Lederer, F.
    Mutation to glutamine of histidine 373, the catalytic base of flavocytochrome b2 (L-lactate dehydrogenase) (1995), Biochimie, 77, 621-630.
    View publication on PubMed

Cloned(Commentary)

Cloned (Comment) Organism
in Escherichia coli MM294 Saccharomyces cerevisiae

Crystallization (Commentary)

Crystallization (Comment) Organism
crystallization of Y143F mutant, vapor diffusion technique in presence of PEG 4000 Saccharomyces cerevisiae

Protein Variants

Protein Variants Comment Organism
H373Q mutant Saccharomyces cerevisiae
Y143F turnover reduced to 15% Saccharomyces cerevisiae
Y254L only traces of activity Saccharomyces cerevisiae

Inhibitors

Inhibitors Comment Organism Structure
Fluoropyruvate
-
Saccharomyces cerevisiae
pyruvate competitive at low concentrations, non-competitve at high concentrations Saccharomyces cerevisiae

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.0001
-
L-lactate Y254L mutant enzyme with 2,6-dichloroindophenol as electron acceptor Saccharomyces cerevisiae
0.037
-
L-lactate wild type enzyme with 2,6-dichloroindophenol as electron acceptor Saccharomyces cerevisiae
0.04
-
L-lactate Y143F mutant enzyme with 2,6-dichloroindophenol as electron acceptor Saccharomyces cerevisiae
0.13
-
L-lactate H373Q mutant enzyme with 2,6-dichloroindophenol as electron acceptor Saccharomyces cerevisiae

Metals/Ions

Metals/Ions Comment Organism Structure
Fe2+ bound to enzyme Saccharomyces cerevisiae

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
(S)-lactate + 2 ferricytochrome c Saccharomyces cerevisiae can feed electrons to respiratory chain at the level of cytochrome c pyruvate + 2 ferrocytochrome c + 2 H+
-
?

Organism

Organism UniProt Comment Textmining
Saccharomyces cerevisiae
-
-
-

Purification (Commentary)

Purification (Comment) Organism
wild type and mutant enzymes Saccharomyces cerevisiae

Storage Stability

Storage Stability Organism
-80°C as saturated solution Saccharomyces cerevisiae
4°C, precipitate from 70% saturated ammonium sulfate, under nitrogen, stable for several weeks Saccharomyces cerevisiae

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
(S)-lactate + 2 ferricytochrome c
-
Saccharomyces cerevisiae pyruvate + 2 ferrocytochrome c + 2 H+
-
?
(S)-lactate + 2 ferricytochrome c can feed electrons to respiratory chain at the level of cytochrome c Saccharomyces cerevisiae pyruvate + 2 ferrocytochrome c + 2 H+
-
?
additional information 2,6-dichlorophenolindophenol used as electron acceptor, 10times more sensitive than ferricyanide Saccharomyces cerevisiae ?
-
?
additional information electron acceptors other than ferricytochrome c used Saccharomyces cerevisiae ?
-
?

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
0.39
-
L-lactate Y254L mutant enzyme Saccharomyces cerevisiae
60
-
L-lactate Y143F mutant enzyme Saccharomyces cerevisiae

Cofactor

Cofactor Comment Organism Structure
additional information electron acceptors other than ferricytochrome c used Saccharomyces cerevisiae
additional information 2,6-dichlorophenolindophenol used as electron acceptor, 10-fold more sensitive than ferricyanide Saccharomyces cerevisiae

Ki Value [mM]

Ki Value [mM] Ki Value maximum [mM] Inhibitor Comment Organism Structure
3
-
pyruvate competitive Saccharomyces cerevisiae
30
-
pyruvate non-competitive Saccharomyces cerevisiae