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Literature summary for 1.1.2.3 extracted from

  • Daff, S.; Manson, F.D.C.; Reid, G.A.; Chapman, S.K.
    Strategic manipulation of the substrate specificity of Saccharomyces cerevisiae flavocytochrome b2 (1994), Biochem. J., 301, 829-834.
No PubMed abstract available

Cloned(Commentary)

Cloned (Comment) Organism
-
Saccharomyces cerevisiae

Protein Variants

Protein Variants Comment Organism
A198G turnover reduced to 50% Saccharomyces cerevisiae
A198G/L230A double mutant, turnover reduced to less than 10% Saccharomyces cerevisiae
L230A turnover reduced to less than 10% Saccharomyces cerevisiae

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.4
-
L-lactate A198G mutant enzyme Saccharomyces cerevisiae
0.5
-
L-lactate wild type enzyme Saccharomyces cerevisiae
0.6
-
L-lactate L230A mutant enzyme Saccharomyces cerevisiae
38
-
L-lactate A198G/L230A mutant enzyme Saccharomyces cerevisiae

Metals/Ions

Metals/Ions Comment Organism Structure
Fe2+ bound to enzyme Saccharomyces cerevisiae

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
(S)-lactate + 2 ferricytochrome c Saccharomyces cerevisiae can feed electrons to respiratory chain at the level of cytochrome c pyruvate + 2 ferrocytochrome c + 2 H+
-
?

Organism

Organism UniProt Comment Textmining
Saccharomyces cerevisiae
-
-
-

Storage Stability

Storage Stability Organism
4°C, precipitate from 70% saturated ammonium sulfate, under nitrogen, stable for several weeks Saccharomyces cerevisiae

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
(S)-2-hydroxybutyrate + ferricytochrome c
-
Saccharomyces cerevisiae 2-oxobutyrate + ferrocytochrome c
-
?
(S)-2-hydroxyhexanoate + ferricytochrome c
-
Saccharomyces cerevisiae 2-oxohexanoate + ferrocytochrome c
-
?
(S)-2-hydroxyoctanoate + ferricytochrome c
-
Saccharomyces cerevisiae 2-oxooctanoate + ferrocytochrome c
-
?
(S)-2-hydroxyvalerate + ferricytochrome c
-
Saccharomyces cerevisiae 2-oxovalerate + ferrocytochrome c
-
?
(S)-lactate + 2 ferricytochrome c
-
Saccharomyces cerevisiae pyruvate + 2 ferrocytochrome c + 2 H+
-
?
(S)-lactate + 2 ferricytochrome c can feed electrons to respiratory chain at the level of cytochrome c Saccharomyces cerevisiae pyruvate + 2 ferrocytochrome c + 2 H+
-
?
glycolate + ferricytochrome c very poor substrate Saccharomyces cerevisiae glyoxylate + ferrocytochrome c
-
?
additional information ferricyanide used as electron acceptor Saccharomyces cerevisiae ?
-
?
additional information electron acceptors other than ferricytochrome c used Saccharomyces cerevisiae ?
-
?

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
additional information
-
additional information systematic determination of substrates with different chain length Saccharomyces cerevisiae
30
-
L-lactate L230A mutant enzyme Saccharomyces cerevisiae
41
-
L-lactate A198G/L230A mutant enzyme Saccharomyces cerevisiae
185
-
L-lactate A198G mutant enzyme Saccharomyces cerevisiae
400
-
L-lactate wild type enzyme Saccharomyces cerevisiae

Cofactor

Cofactor Comment Organism Structure
additional information ferricyanide used as electron acceptor Saccharomyces cerevisiae
additional information electron acceptors other than ferricytochrome c used Saccharomyces cerevisiae