Application | Comment | Organism |
---|---|---|
pharmacology | stereospecific production of (R)-3-quinuclidinol, an important chiral building block for the synthesis of various pharmaceuticals | Agrobacterium tumefaciens |
synthesis | stereospecific production of (R)-3-quinuclidinol, an important chiral building block for the synthesis of various pharmaceuticals | Agrobacterium tumefaciens |
Cloned (Comment) | Organism |
---|---|
overexpressed as a fusion protein with an N-terminal His6 tag in Eschrichia coli Rosetta(DE3) | Agrobacterium tumefaciens |
Crystallization (Comment) | Organism |
---|---|
crystallized under a reservoir solution condition of 0.2 M ammonium acetate, 0.1 M HEPES pH 8.5 and 24% (w/v) PEG3350 | Agrobacterium tumefaciens |
Protein Variants | Comment | Organism |
---|---|---|
D40A | no activity | Agrobacterium tumefaciens |
E197A | no activity | Agrobacterium tumefaciens |
R196A | activity is 76% compared to activity of wild-type enzyme | Agrobacterium tumefaciens |
Y216V | activity is 31% compared to activity of wild-type enzyme | Agrobacterium tumefaciens |
General Stability | Organism |
---|---|
NADH contributes to the stability of the alpha7 helix of the enzyme | Agrobacterium tumefaciens |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Agrobacterium tumefaciens | G1K3P5 | - |
- |
Purification (Comment) | Organism |
---|---|
- |
Agrobacterium tumefaciens |
Synonyms | Comment | Organism |
---|---|---|
AtQR | - |
Agrobacterium tumefaciens |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
37 | - |
assay at | Agrobacterium tumefaciens |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
7 | - |
assay at | Agrobacterium tumefaciens |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
NADH | - |
Agrobacterium tumefaciens |