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Literature summary for 1.1.1.B3 extracted from

  • Xu, G.C.; Shang, Y.P.; Yu, H.L.; Xu, J.H.
    Identification of key residues in Debaryomyces hansenii carbonyl reductase for highly productive preparation of (S)-aryl halohydrins (2015), Chem. Commun. (Camb.), 51, 15728-15731.
    View publication on PubMed
Search for more literature for 1.1.1.B3

Crystallization (Commentary)

Crystallization (Comment) Organism
homology modeling of structure. The substrate binding pocket consists of residues P122, W123, E125, S174, N179, V180, S214, P215, G217, Y218, I223, S224, D225, F226 and V227, with all of the residues lying in the flexible loop regions Debaryomyces hansenii

Protein Variants

Protein Variants Comment Organism
N179S about 40fold increase in specific activity Debaryomyces hansenii
N179S/I214F/S215G about 50fold increase in specific activity Debaryomyces hansenii
S174G about 7fold increase in specific activity Debaryomyces hansenii

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.4
-
 1-chloroacetophenone mutant N179S, pH 6.5, 30°C Debaryomyces hansenii
1.31
-
 1-chloroacetophenone mutant S174G, pH 6.5, 30°C Debaryomyces hansenii
1.45
-
 1-chloroacetophenone mutant N179S/I214F/S215G, pH 6.5, 30°C Debaryomyces hansenii
2.25
-
 1-chloroacetophenone wild-type, pH 6.5, 30°C Debaryomyces hansenii

Organism

Organism UniProt Comment Textmining
Debaryomyces hansenii
-
-
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1-chloroacetophenone + NADH + H+
-
 Debaryomyces hansenii (1S)-2-chloro-1-phenylethanol + NAD+ more thasn 99% enantiomeric excess ?

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
3.95
-
 1-chloroacetophenone wild-type, pH 6.5, 30°C Debaryomyces hansenii
21.8
-
 1-chloroacetophenone mutant S174G, pH 6.5, 30°C Debaryomyces hansenii
119
-
 1-chloroacetophenone mutant N179S, pH 6.5, 30°C Debaryomyces hansenii
204
-
 1-chloroacetophenone mutant N179S/I214F/S215G, pH 6.5, 30°C Debaryomyces hansenii

kcat/KM [mM/s]

kcat/KM Value [1/mMs-1] kcat/KM Value Maximum [1/mMs-1] Substrate Comment Organism Structure
1.75
-
 1-chloroacetophenone wild-type, pH 6.5, 30°C Debaryomyces hansenii
14.2
-
 1-chloroacetophenone mutant S174G, pH 6.5, 30°C Debaryomyces hansenii
140
-
 1-chloroacetophenone mutant N179S/I214F/S215G, pH 6.5, 30°C Debaryomyces hansenii
295
-
 1-chloroacetophenone mutant N179S, pH 6.5, 30°C Debaryomyces hansenii