Any feedback?
Please rate this page
(literature.php)
(0/150)

BRENDA support

Literature summary for 1.1.1.B20 extracted from

  • Hao, W.; Ji, F.; Wang, J.; Zhang, Y.; Wang, T.; Bao, Y.
    Biochemical characterization of unusual meso-2,3-butanediol dehydrogenase from a strain of Bacillus subtilis (2014), J. Mol. Catal. B, 109, 184-190 .
No PubMed abstract available
Search for more literature for 1.1.1.B20

Cloned(Commentary)

Cloned (Comment) Organism
gene budC , recombinant expression in Escherichia coli strain BL21(DE3) Klebsiella aerogenes
gene budC, recombinant expression of wild-type and mutant enzymes in Escherichia coli strain BL21(DE3) Bacillus subtilis

Protein Variants

Protein Variants Comment Organism
D194G site-directed mutagenesis, the mutant binds the substrate but is catalytically almost inactive. The mutant is inactive with (2S,3S)-butanediol, meso-butanediol and (2R,3R)-butanediol. D194G enzyme mutant shows a similar secondary structure compared to Enterobacter aerogenes BDH. While the mutant is highly susceptible to protease digestion compared to the wild-type enzyme. Homology modeling of the mutant enzyme, with meso-2,3-butanediol dehydrogenase from Klebsiella pneumoniae, PDB ID 1GEG, as a template, reveals that Gly194 seems to lose the hydrogen bond interactions with the surrounding residues (Gly206, Gly207 and Thr209), resulting in a putative conformational changes of mutant D194G which might be responsible for the loss of activity Bacillus subtilis

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
additional information
-
 additional information thermodynamics Bacillus subtilis
additional information
-
 additional information thermodynamics Klebsiella aerogenes

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
(R)-acetoin + NADH + H+ Bacillus subtilis
-
 meso-2,3-butanediol + NAD+
-
 r
(R)-acetoin + NADH + H+ Klebsiella aerogenes
-
 meso-2,3-butanediol + NAD+
-
 r
(R)-acetoin + NADH + H+ Klebsiella aerogenes KCTC 2190
-
 meso-2,3-butanediol + NAD+
-
 r
meso-2,3-butanediol + NAD+ Bacillus subtilis
-
 (R)-acetoin + NADH + H+
-
 r
meso-2,3-butanediol + NAD+ Klebsiella aerogenes
-
 (R)-acetoin + NADH + H+
-
 r
meso-2,3-butanediol + NAD+ Klebsiella aerogenes KCTC 2190
-
 (R)-acetoin + NADH + H+
-
 r

Organism

Organism UniProt Comment Textmining
Bacillus subtilis
-
-
-
Klebsiella aerogenes A0A0H3FZT7
-
-
Klebsiella aerogenes KCTC 2190 A0A0H3FZT7
-
-

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
2.37
-
 purified recombinant enzyme, pH 7.6, 37°C, substrates meso-2,3-butanediol and NADP+ Klebsiella aerogenes
2.9
-
 purified recombinant enzyme, pH 7.6, 37°C, substrates acetoin and NADPH Klebsiella aerogenes
119.55
-
 purified recombinant enzyme, pH 7.6, 37°C, substrates meso-2,3-butanediol and NAD+ Klebsiella aerogenes
461.67
-
 purified recombinant enzyme, pH 7.6, 37°C, substrates acetoin and NADH Klebsiella aerogenes

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
(R)-acetoin + NADH + H+
-
 Bacillus subtilis meso-2,3-butanediol + NAD+
-
 r
(R)-acetoin + NADH + H+
-
 Klebsiella aerogenes meso-2,3-butanediol + NAD+
-
 r
(R)-acetoin + NADH + H+ preferred reaction direction Klebsiella aerogenes meso-2,3-butanediol + NAD+
-
 r
(R)-acetoin + NADH + H+
-
 Klebsiella aerogenes KCTC 2190 meso-2,3-butanediol + NAD+
-
 r
(R)-acetoin + NADH + H+ preferred reaction direction Klebsiella aerogenes KCTC 2190 meso-2,3-butanediol + NAD+
-
 r
(R)-acetoin + NADPH + H+ very low activity Klebsiella aerogenes meso-2,3-butanediol + NADP+
-
 r
(R)-acetoin + NADPH + H+ very low activity Klebsiella aerogenes KCTC 2190 meso-2,3-butanediol + NADP+
-
 r
meso-2,3-butanediol + NAD+
-
 Bacillus subtilis (R)-acetoin + NADH + H+
-
 r
meso-2,3-butanediol + NAD+
-
 Klebsiella aerogenes (R)-acetoin + NADH + H+
-
 r
meso-2,3-butanediol + NAD+
-
 Klebsiella aerogenes KCTC 2190 (R)-acetoin + NADH + H+
-
 r
meso-2,3-butanediol + NADP+ very low activity Klebsiella aerogenes (R)-acetoin + NADPH + H+
-
 r
additional information meso-2,3-butanediol dehydrogenase (BDH) catalyzes the redox reaction between (R)-acetoin and meso-2,3-butanediol Bacillus subtilis ?
-
 ?
additional information the enzyme is also active with diacetyl and NADH Klebsiella aerogenes ?
-
 ?
additional information the enzyme is also active with diacetyl and NADH Klebsiella aerogenes KCTC 2190 ?
-
 ?

Synonyms

Synonyms Comment Organism
BDH
-
 Bacillus subtilis
BDH
-
 Klebsiella aerogenes
budC
-
 Bacillus subtilis
budC
-
 Klebsiella aerogenes
meso-2,3-BDH
-
 Bacillus subtilis
meso-2,3-BDH
-
 Klebsiella aerogenes

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
37
-
 assay at Bacillus subtilis
37
-
 assay at Klebsiella aerogenes

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
7.6
-
 assay at Bacillus subtilis
7.6
-
 assay at Klebsiella aerogenes

Cofactor

Cofactor Comment Organism Structure
additional information the enzyme prefers NAD+as a coenzyme Klebsiella aerogenes
NAD+
-
 Bacillus subtilis
NAD+
-
 Klebsiella aerogenes
NADH
-
 Bacillus subtilis
NADH
-
 Klebsiella aerogenes
NADP+ very low activity Klebsiella aerogenes
NADPH very low activity Klebsiella aerogenes