Any feedback?
Please rate this page
(literature.php)
(0/150)

BRENDA support

Literature summary for 1.1.1.B20 extracted from

  • Zhang, L.; Xu, Q.; Zhan, S.; Li, Y.; Lin, H.; Sun, S.; Sha, L.; Hu, K.; Guan, X.; Shen, Y.
    A new NAD(H)-dependent meso-2,3-butanediol dehydrogenase from an industrially potential strain Serratia marcescens H30 (2014), Appl. Microbiol. Biotechnol., 98, 1175-1184.
    View publication on PubMed
Search for more literature for 1.1.1.B20

Cloned(Commentary)

Cloned (Comment) Organism
gene budC, sequence comparisons, expression in Escherichia coli strain BL21(DE3), subcloning in Escherichia coli strain DH5alpha Serratia marcescens

Inhibitors

Inhibitors Comment Organism Structure
CaCl2
-
 Serratia marcescens
Fe3+ inhibits diacetyl and (3S/3R)-acetoin reduction Serratia marcescens
FeCl3
-
 Serratia marcescens
NaCl
-
 Serratia marcescens
NH4Cl
-
 Serratia marcescens
ZnSO4
-
 Serratia marcescens

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.97
-
 (3R)-acetoin pH 8.0, 40°C Serratia marcescens
3.3
-
 diacetyl pH 8.0, 40°C Serratia marcescens
4.1
-
 meso-2,3-butanediol pH 5.0, 40°C Serratia marcescens
31.2
-
 (2S,3S)-2,3-butanediol pH 5.0, 40°C Serratia marcescens

Metals/Ions

Metals/Ions Comment Organism Structure
Fe2+ enhances for oxidation and reduction reactions Serratia marcescens
Mg2+ enhances diacetyl and (3S/3R)-acetoin reduction Serratia marcescens
Mn2+ enhances diacetyl and (3S/3R)-acetoin reduction Serratia marcescens

Organism

Organism UniProt Comment Textmining
Serratia marcescens H9XP47 gene budC
-
Serratia marcescens H30 H9XP47 gene budC
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
(2S,3S)-2,3-butanediol + NAD+ low activity Serratia marcescens (3S)-acetoin + NADH + H+
-
 r
(2S,3S)-2,3-butanediol + NAD+ low activity Serratia marcescens H30 (3S)-acetoin + NADH + H+
-
 r
(3R)-acetoin + NADH + H+
-
 Serratia marcescens meso-2,3-butanediol + NAD+
-
 r
(3R)-acetoin + NADH + H+
-
 Serratia marcescens H30 meso-2,3-butanediol + NAD+
-
 r
(3S)-acetoin + NADH + H+ at pH 9.0 Serratia marcescens (2S,3S)-2,3-butanediol + NAD+
-
 r
(3S)-acetoin + NADH + H+ at pH 9.0 Serratia marcescens H30 (2S,3S)-2,3-butanediol + NAD+
-
 r
1,2-pentanediol + NADH + H+ low activity Serratia marcescens ?
-
 ?
1,2-pentanediol + NADH + H+ low activity Serratia marcescens H30 ?
-
 ?
1,2-propandiol + NADH + H+ low activity Serratia marcescens ?
-
 ?
diacetyl + NADH + H+
-
 Serratia marcescens (3S)-acetoin + NAD+
-
 ?
glycerol + NADH + H+ low activity Serratia marcescens ?
-
 ?
meso-2,3-butanediol + NAD+
-
 Serratia marcescens (3R)-acetoin + NADH + H+
-
 r
additional information (2R,3R)-2,3-butanediol is no substrate for the enzyme Serratia marcescens ?
-
 ?
additional information (2R,3R)-2,3-butanediol is no substrate for the enzyme Serratia marcescens H30 ?
-
 ?

Synonyms

Synonyms Comment Organism
BDH
-
 Serratia marcescens
NAD(H)-dependent meso-2,3-BDH
-
 Serratia marcescens
NAD(H)-dependent meso-2,3-butanediol dehydrogenase
-
 Serratia marcescens

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
40
-
 optimum for oxidation and reduction reactions Serratia marcescens

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
1.02
-
 (2S,3S)-2,3-butanediol pH 5.0, 40°C Serratia marcescens
6.2
-
 meso-2,3-butanediol pH 5.0, 40°C Serratia marcescens
11.5
-
 diacetyl pH 8.0, 40°C Serratia marcescens
19.7
-
 (3R)-acetoin pH 8.0, 40°C Serratia marcescens

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
5
-
 maximal activity of (3S/3R)-acetoin reduction Serratia marcescens
8
-
 maximal activity of diacetyl reduction and meso-2,3-butanediol oxidation Serratia marcescens

Cofactor

Cofactor Comment Organism Structure
NAD+
-
 Serratia marcescens
NADH
-
 Serratia marcescens

General Information

General Information Comment Organism
evolution the enzyme belongs to the shortchain dehydrogenase/reductase superfamily Serratia marcescens
additional information the enzyme possesses two conserved sequences including the coenzyme binding motif (GxxxGxG) and the active-site motif (YxxxK) Serratia marcescens