Any feedback?
Literature summary for 1.1.1.94 extracted from
- Biosynthesis in Escherichia coli of sn-glycerol-3-phosphate, a precursor of phospholipid. Further kinetic characterization of wild type and feedback-resistant forms of the biosynthetic sn-glycerol-3-phosphate dehydrogenase (1980), J. Biol. Chem., 255, 3492-3497.
Protein Variants
| Protein Variants | Comment | Organism |
|---|---|---|
| additional information | existence of a naturally occurring feedback inhibition-resistant mutant | Escherichia coli |
Inhibitors
| Inhibitors | Comment | Organism | Structure |
|---|---|---|---|
| 2',5'-ADP | - |
Escherichia coli |  |
| 3,4-dihydroxybutyl 1-phosphonate | competitive versus dihydroxyacetone phosphate, binding to a regulatory site | Escherichia coli | |
| ADP | - |
Escherichia coli | |
| ADP-ribose | - |
Escherichia coli | |
| AMP | - |
Escherichia coli | |
| ATP | - |
Escherichia coli | |
| ethylene glycol phosphate | binding to the active site | Escherichia coli | |
| NAD+ | - |
Escherichia coli | |
| NADP+ | - |
Escherichia coli | |
| NMN | - |
Escherichia coli | |
| sn-glycerol-3-phosphate | the wild-type enzyme is feedback regulated, competitive versus dihydroxyacetone phosphate, binding to a regulatory site | Escherichia coli | |
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.0037 | - |
NADPH | pH 7.4, 23°C, feedback-resistant mutant enzyme | Escherichia coli | |
| 0.004 | - |
NADH | pH 7.4, 23°C, feedback-resistant mutant enzyme | Escherichia coli | |
| 0.0041 | - |
NADPH | pH 7.4, 23°C, wild-type mutant enzyme | Escherichia coli | |
| 0.0045 | - |
NADH | pH 7.4, 23°C, wild-type mutant enzyme | Escherichia coli | |
| 0.005 | - |
reduced nicotinamide hypoxanthine dinucleotide | pH 7.4, 23°C, wild-type mutant enzyme and feedback-resistant mutant enzyme | Escherichia coli | |
Natural Substrates/ Products (Substrates)
| Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| glycerone phosphate + NAD(P)H | Escherichia coli | - |
sn-glycerol-3-phosphate + NAD(P)+ | - |
r | |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Escherichia coli | - |
- |
- |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| glycerone phosphate + NAD(P)H | - |
Escherichia coli | sn-glycerol-3-phosphate + NAD(P)+ | - |
r | |
| glycerone phosphate + NAD(P)H | i.e. dihydroxyacetone phosphate | Escherichia coli | sn-glycerol-3-phosphate + NAD(P)+ | product analysis | r | |
| glycerone phosphate + NADH + H+ | - |
Escherichia coli | sn-glycerol 3-phosphate + NAD+ | - |
? | |
| glycerone phosphate + reduced nicotinamide hypoxanthine dinucleotide | - |
Escherichia coli | sn-glycerol-3-phosphate + oxidized nicotinamide hypoxanthine dinucleotide | - |
? | |
Synonyms
| Synonyms | Comment | Organism |
|---|---|---|
| sn-glycerol-3-phosphate dehydrogenase | - |
Escherichia coli |
Temperature Optimum [°C]
| Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 23 | - |
assay at | Escherichia coli |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 7.4 | - |
assay at | Escherichia coli |
Cofactor
| Cofactor | Comment | Organism | Structure |
|---|---|---|---|
| NAD+ | - |
Escherichia coli | |
| NADH | - |
Escherichia coli | |
| NADP+ | B-type specificity of wild-type and feedback-resistant mutant enzyme | Escherichia coli | |
| NADPH | B-type specificity of wild-type and feedback-resistant mutant enzyme | Escherichia coli | |
| nicotinamide hypoxanthine dinucleotide | - |
Escherichia coli | |
Ki Value [mM]
| Ki Value [mM] | Ki Value maximum [mM] | Inhibitor | Comment | Organism | Structure |
|---|---|---|---|---|---|
| additional information | - |
additional information | product inhibition kinetics | Escherichia coli | |
| 0.1 | - |
ADP-ribose | pH 7.4, 23°C, wild-type enzyme | Escherichia coli | |
| 0.19 | - |
NADP+ | pH 7.4, 23°C, feedback-resistant mutant enzyme | Escherichia coli | |
| 0.2 | - |
NAD+ | pH 7.4, 23°C, wild-type enzyme | Escherichia coli | |
| 0.21 | - |
ADP-ribose | pH 7.4, 23°C, feedback-resistant mutant enzyme | Escherichia coli | |
| 0.22 | - |
NAD+ | pH 7.4, 23°C, feedback-resistant mutant enzyme | Escherichia coli | |
| 0.25 | - |
NADP+ | pH 7.4, 23°C, wild-type enzyme | Escherichia coli | |
| 0.7 | - |
ADP | pH 7.4, 23°C, feedback-resistant mutant enzyme | Escherichia coli | |
| 0.8 | - |
ADP | pH 7.4, 23°C, wild-type enzyme | Escherichia coli | |
| 1.4 | - |
ethylene glycol phosphate | pH 7.4, 23°C, wild-type and feedback-resistant mutant enzyme | Escherichia coli | |
| 1.4 | - |
ATP | pH 7.4, 23°C, wild-type enzyme and feedback-resistant mutant enzyme | Escherichia coli | |
| 1.8 | - |
2',5'-ADP | pH 7.4, 23°C, wild-type enzyme | Escherichia coli | |
| 2 | - |
2',5'-ADP | pH 7.4, 23°C, feedback-resistant mutant enzyme | Escherichia coli | |
| 4.8 | - |
AMP | pH 7.4, 23°C, feedback-resistant mutant enzyme | Escherichia coli | |
| 5 | - |
AMP | pH 7.4, 23°C, wild-type enzyme | Escherichia coli | |
| 10 | - |
NMN | pH 7.4, 23°C, wild-type enzyme and feedback-resistant mutant enzyme | Escherichia coli | |
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