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Literature summary for 1.1.1.9 extracted from

  • Morimoto, S.; Matsuo, M.; Azuma, K.; Sinskey, A.J.
    Purification and properties of D-xylulose reductase from Pachysolen tannophilus (1986), J. Ferment. Technol., 64, 219-225.
No PubMed abstract available

Activating Compound

Activating Compound Comment Organism Structure
cysteine slight activation Pachysolen tannophilus
glycine slight activation Pachysolen tannophilus
GSH activation Pachysolen tannophilus

General Stability

General Stability Organism
freezing/thawing inactivates, 8 cycles lead to 89% loss of activity and 11 cycles to complete inactivation Pachysolen tannophilus

Inhibitors

Inhibitors Comment Organism Structure
AgNO3
-
Pachysolen tannophilus
CuSO4
-
Pachysolen tannophilus
cysteine at 294 mM Pachysolen tannophilus
FeSO4
-
Pachysolen tannophilus
HgCl2 complete inhibition Pachysolen tannophilus
iodoacetate complete inhibition Pachysolen tannophilus
MnSO4
-
Pachysolen tannophilus
NaCl at 160 mM Pachysolen tannophilus
NH4Cl at 310 mM Pachysolen tannophilus
Pb(CH3COO)2
-
Pachysolen tannophilus
ZnSO4 complete inhibition Pachysolen tannophilus

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
11
-
xylitol
-
Pachysolen tannophilus
30
-
D-sorbitol
-
Pachysolen tannophilus
50
-
ribitol
-
Pachysolen tannophilus

Localization

Localization Comment Organism GeneOntology No. Textmining
cytoplasm
-
Pachysolen tannophilus 5737
-

Metals/Ions

Metals/Ions Comment Organism Structure
KCl slight activation Pachysolen tannophilus
Mg2+
-
Pachysolen tannophilus
NaCl activation Pachysolen tannophilus
NH4Cl activation Pachysolen tannophilus

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
40000
-
4 * 40000, SDS-PAGE Pachysolen tannophilus
120000
-
gel filtration Pachysolen tannophilus

Organism

Organism UniProt Comment Textmining
Pachysolen tannophilus
-
-
-

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
29.9
-
-
Pachysolen tannophilus

Storage Stability

Storage Stability Organism
-15°C, 60% loss of activity after 1 month Pachysolen tannophilus
-2°C, purified stable for a month Pachysolen tannophilus
0°C, 34% loss of activity within a month Pachysolen tannophilus

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
D-ribitol + NAD+ 85% of the rate of xylitol oxidation Pachysolen tannophilus D-ribulose + NADH
-
?
D-sorbitol + NAD+ rate of xylitol oxidation at 95% Pachysolen tannophilus D-fructose + NADH + H+
-
?
glycerol + NAD+ poor substrate Pachysolen tannophilus ?
-
?
additional information no oxidation of inositol, meso-erythritol and D-(+)-arabitol Pachysolen tannophilus ?
-
?
additional information no oxidation of mannitol Pachysolen tannophilus ?
-
?
xylitol + NAD+
-
Pachysolen tannophilus D-xylulose + NADH + H+
-
?

Subunits

Subunits Comment Organism
tetramer 4 * 40000, SDS-PAGE Pachysolen tannophilus

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
55
-
-
Pachysolen tannophilus

Temperature Range [°C]

Temperature Minimum [°C] Temperature Maximum [°C] Comment Organism
35 68 about half-maximal activity at 35°C and 68°C Pachysolen tannophilus

Temperature Stability [°C]

Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
25
-
and below stable Pachysolen tannophilus
60
-
complete inactivation within 5 min Pachysolen tannophilus

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
9.1 10 substrate oxidation, plateau Pachysolen tannophilus

pH Range

pH Minimum pH Maximum Comment Organism
8.7 10.5 about 80% of maximal oxidation activity at pH 8.7 and 10.5 Pachysolen tannophilus

pH Stability

pH Stability pH Stability Maximum Comment Organism
7 9 stable Pachysolen tannophilus

Cofactor

Cofactor Comment Organism Structure
additional information no oxidation of ribitol or sorbitol with NADP+ Pachysolen tannophilus
NAD+
-
Pachysolen tannophilus