Activating Compound | Comment | Organism | Structure |
---|---|---|---|
cysteine | slight activation | Pachysolen tannophilus | |
glycine | slight activation | Pachysolen tannophilus | |
GSH | activation | Pachysolen tannophilus |
General Stability | Organism |
---|---|
freezing/thawing inactivates, 8 cycles lead to 89% loss of activity and 11 cycles to complete inactivation | Pachysolen tannophilus |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
AgNO3 | - |
Pachysolen tannophilus | |
CuSO4 | - |
Pachysolen tannophilus | |
cysteine | at 294 mM | Pachysolen tannophilus | |
FeSO4 | - |
Pachysolen tannophilus | |
HgCl2 | complete inhibition | Pachysolen tannophilus | |
iodoacetate | complete inhibition | Pachysolen tannophilus | |
MnSO4 | - |
Pachysolen tannophilus | |
NaCl | at 160 mM | Pachysolen tannophilus | |
NH4Cl | at 310 mM | Pachysolen tannophilus | |
Pb(CH3COO)2 | - |
Pachysolen tannophilus | |
ZnSO4 | complete inhibition | Pachysolen tannophilus |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
11 | - |
xylitol | - |
Pachysolen tannophilus | |
30 | - |
D-sorbitol | - |
Pachysolen tannophilus | |
50 | - |
ribitol | - |
Pachysolen tannophilus |
Localization | Comment | Organism | GeneOntology No. | Textmining |
---|---|---|---|---|
cytoplasm | - |
Pachysolen tannophilus | 5737 | - |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
KCl | slight activation | Pachysolen tannophilus | |
Mg2+ | - |
Pachysolen tannophilus | |
NaCl | activation | Pachysolen tannophilus | |
NH4Cl | activation | Pachysolen tannophilus |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
40000 | - |
4 * 40000, SDS-PAGE | Pachysolen tannophilus |
120000 | - |
gel filtration | Pachysolen tannophilus |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Pachysolen tannophilus | - |
- |
- |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
29.9 | - |
- |
Pachysolen tannophilus |
Storage Stability | Organism |
---|---|
-15°C, 60% loss of activity after 1 month | Pachysolen tannophilus |
-2°C, purified stable for a month | Pachysolen tannophilus |
0°C, 34% loss of activity within a month | Pachysolen tannophilus |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
D-ribitol + NAD+ | 85% of the rate of xylitol oxidation | Pachysolen tannophilus | D-ribulose + NADH | - |
? | |
D-sorbitol + NAD+ | rate of xylitol oxidation at 95% | Pachysolen tannophilus | D-fructose + NADH + H+ | - |
? | |
glycerol + NAD+ | poor substrate | Pachysolen tannophilus | ? | - |
? | |
additional information | no oxidation of inositol, meso-erythritol and D-(+)-arabitol | Pachysolen tannophilus | ? | - |
? | |
additional information | no oxidation of mannitol | Pachysolen tannophilus | ? | - |
? | |
xylitol + NAD+ | - |
Pachysolen tannophilus | D-xylulose + NADH + H+ | - |
? |
Subunits | Comment | Organism |
---|---|---|
tetramer | 4 * 40000, SDS-PAGE | Pachysolen tannophilus |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
55 | - |
- |
Pachysolen tannophilus |
Temperature Minimum [°C] | Temperature Maximum [°C] | Comment | Organism |
---|---|---|---|
35 | 68 | about half-maximal activity at 35°C and 68°C | Pachysolen tannophilus |
Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|
25 | - |
and below stable | Pachysolen tannophilus |
60 | - |
complete inactivation within 5 min | Pachysolen tannophilus |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
9.1 | 10 | substrate oxidation, plateau | Pachysolen tannophilus |
pH Minimum | pH Maximum | Comment | Organism |
---|---|---|---|
8.7 | 10.5 | about 80% of maximal oxidation activity at pH 8.7 and 10.5 | Pachysolen tannophilus |
pH Stability | pH Stability Maximum | Comment | Organism |
---|---|---|---|
7 | 9 | stable | Pachysolen tannophilus |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
additional information | no oxidation of ribitol or sorbitol with NADP+ | Pachysolen tannophilus | |
NAD+ | - |
Pachysolen tannophilus |