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Literature summary for 1.1.1.86 extracted from

  • Oh, B.R.; Heo, S.Y.; Lee, S.M.; Hong, W.K.; Park, J.M.; Jung, Y.R.; Kim, D.H.; Sohn, J.H.; Seo, J.W.; Kim, C.H.
    Production of 2-butanol from crude glycerol by a genetically-engineered Klebsiella pneumoniae strain (2014), Biotechnol. Lett., 36, 57-62.
    View publication on PubMed

Application

Application Comment Organism
biofuel production engineering of Klebsiella pneumoniae to produce 2-butanol from crude glycerol as a sole carbon source by expressing acetolactate synthase (ilvIH), keto-acid reducto-isomerase (ilvC) and dihydroxyacid dehydratase (ilvD) from Klebsiella pneumoniae, and aalpha-ketoisovalerate decarboxylase (kivd) and alcohol dehydrogenase (adhA) from Lactococcus lactis. The engineered strain produces 2-butanol (160 mg/l) from crude glycerol. Elimination of the 2,3-butanediol pathway from the recombinant strain by inactivating alpha-acetolactate decarboxylase (adc) improves the yield of 2-butanol 320 mg/l Klebsiella pneumoniae subsp. pneumoniae

Organism

Organism UniProt Comment Textmining
Klebsiella pneumoniae subsp. pneumoniae A6TGG1
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Klebsiella pneumoniae subsp. pneumoniae ATCC 700721 A6TGG1
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Synonyms

Synonyms Comment Organism
ilvC
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Klebsiella pneumoniae subsp. pneumoniae