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Literature summary for 1.1.1.85 extracted from

  • Hamajima, Y.; Nagae, T.; Watanabe, N.; Ohmae, E.; Kato-Yamada, Y.; Kato, C.
    Pressure adaptation of 3-isopropylmalate dehydrogenase from an extremely piezophilic bacterium is attributed to a single amino acid substitution (2016), Extremophiles, 20, 177-186.
    View publication on PubMed
Search for more literature for 1.1.1.85

Cloned(Commentary)

Cloned (Comment) Organism
expressed in Escherichia coli HB101 cells Shewanella benthica
expressed in Escherichia coli HB101 cells Shewanella oneidensis

Protein Variants

Protein Variants Comment Organism
A266S the mutant shows reduced catalytic efficiency compared to the wild type enzyme Shewanella benthica
A268I the mutant shows reduced catalytic efficiency compared to the wild type enzyme Shewanella oneidensis
I268A the mutant shows reduced catalytic efficiency compared to the wild type enzyme Shewanella benthica
L106M the mutant shows increased catalytic efficiency compared to the wild type enzyme Shewanella oneidensis
M106L the mutant shows reduced catalytic efficiency compared to the wild type enzyme Shewanella benthica
S266A the mutant shows reduced catalytic efficiency compared to the wild type enzyme Shewanella oneidensis

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.0122
-
 (2R,3S)-3-isopropylmalate mutant enzyme L106M, at pH 7.6 and 25°C Shewanella oneidensis
0.0122
-
 (2R,3S)-3-isopropylmalate wild type enzyme, at pH 7.6 and 25°C Shewanella benthica
0.0141
-
 (2R,3S)-3-isopropylmalate wild type enzyme, at pH 7.6 and 25°C Shewanella oneidensis
0.0226
-
 (2R,3S)-3-isopropylmalate mutant enzyme S266A, at pH 7.6 and 25°C Shewanella oneidensis
0.0239
-
 (2R,3S)-3-isopropylmalate mutant enzyme M106L, at pH 7.6 and 25°C Shewanella benthica
0.0344
-
 (2R,3S)-3-isopropylmalate mutant enzyme A266S, at pH 7.6 and 25°C Shewanella benthica
0.0345
-
 (2R,3S)-3-isopropylmalate mutant enzyme A268I, at pH 7.6 and 25°C Shewanella oneidensis
0.0356
-
 (2R,3S)-3-isopropylmalate mutant enzyme I268A, at pH 7.6 and 25°C Shewanella benthica
0.0708
-
 NAD+ mutant enzyme L106M, at pH 7.6 and 25°C Shewanella oneidensis
0.0756
-
 NAD+ mutant enzyme S266A, at pH 7.6 and 25°C Shewanella oneidensis
0.079
-
 NAD+ mutant enzyme M106L, at pH 7.6 and 25°C Shewanella benthica
0.0814
-
 NAD+ wild type enzyme, at pH 7.6 and 25°C Shewanella oneidensis
0.1058
-
 NAD+ wild type enzyme, at pH 7.6 and 25°C Shewanella benthica
0.1707
-
 NAD+ mutant enzyme I268A, at pH 7.6 and 25°C Shewanella benthica
0.2379
-
 NAD+ mutant enzyme A266S, at pH 7.6 and 25°C Shewanella benthica
0.2785
-
 NAD+ mutant enzyme A268I, at pH 7.6 and 25°C Shewanella oneidensis

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
(2R,3S)-3-isopropylmalate + NAD+ Shewanella benthica
-
 (2S)-2-isopropyl-3-oxosuccinate + NADH + H+
-
 ?
(2R,3S)-3-isopropylmalate + NAD+ Shewanella oneidensis
-
 (2S)-2-isopropyl-3-oxosuccinate + NADH + H+
-
 ?
(2R,3S)-3-isopropylmalate + NAD+ Shewanella benthica DB21MT-2
-
 (2S)-2-isopropyl-3-oxosuccinate + NADH + H+
-
 ?
(2R,3S)-3-isopropylmalate + NAD+ Shewanella oneidensis MR-1 / ATCC 700550
-
 (2S)-2-isopropyl-3-oxosuccinate + NADH + H+
-
 ?

Organism

Organism UniProt Comment Textmining
Shewanella benthica
-
-
-
Shewanella benthica DB21MT-2
-
-
-
Shewanella oneidensis Q8E9N3
-
-
Shewanella oneidensis MR-1 / ATCC 700550 Q8E9N3
-
-

Purification (Commentary)

Purification (Comment) Organism
Ni Sepharose 6 column chromatography Shewanella benthica
Ni Sepharose 6 column chromatography Shewanella oneidensis

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
(2R,3S)-3-isopropylmalate + NAD+
-
 Shewanella benthica (2S)-2-isopropyl-3-oxosuccinate + NADH + H+
-
 ?
(2R,3S)-3-isopropylmalate + NAD+
-
 Shewanella oneidensis (2S)-2-isopropyl-3-oxosuccinate + NADH + H+
-
 ?
(2R,3S)-3-isopropylmalate + NAD+
-
 Shewanella benthica DB21MT-2 (2S)-2-isopropyl-3-oxosuccinate + NADH + H+
-
 ?
(2R,3S)-3-isopropylmalate + NAD+
-
 Shewanella oneidensis MR-1 / ATCC 700550 (2S)-2-isopropyl-3-oxosuccinate + NADH + H+
-
 ?

Synonyms

Synonyms Comment Organism
IPMDH
-
 Shewanella benthica
IPMDH
-
 Shewanella oneidensis

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
2 3.7 NAD+ mutant enzyme A266S, at pH 7.6 and 25°C Shewanella benthica
9.6
-
 (2R,3S)-3-isopropylmalate mutant enzyme I268A, at pH 7.6 and 25°C Shewanella benthica
10.3
-
 NAD+ mutant enzyme I268A, at pH 7.6 and 25°C Shewanella benthica
14.8
-
 (2R,3S)-3-isopropylmalate mutant enzyme M106L, at pH 7.6 and 25°C Shewanella benthica
15.2
-
 NAD+ mutant enzyme M106L, at pH 7.6 and 25°C Shewanella benthica
15.6
-
 NAD+ mutant enzyme S266A, at pH 7.6 and 25°C Shewanella oneidensis
16.1
-
 (2R,3S)-3-isopropylmalate mutant enzyme A268I, at pH 7.6 and 25°C Shewanella oneidensis
16.4
-
 (2R,3S)-3-isopropylmalate wild type enzyme, at pH 7.6 and 25°C Shewanella benthica
16.7
-
 (2R,3S)-3-isopropylmalate mutant enzyme S266A, at pH 7.6 and 25°C Shewanella oneidensis
17.1
-
 NAD+ wild type enzyme, at pH 7.6 and 25°C Shewanella oneidensis
17.4
-
 NAD+ mutant enzyme A268I, at pH 7.6 and 25°C Shewanella oneidensis
17.4
-
 (2R,3S)-3-isopropylmalate wild type enzyme, at pH 7.6 and 25°C Shewanella oneidensis
18.7
-
 NAD+ wild type enzyme, at pH 7.6 and 25°C Shewanella benthica
20.4
-
 (2R,3S)-3-isopropylmalate mutant enzyme A266S, at pH 7.6 and 25°C Shewanella benthica
48.1
-
 (2R,3S)-3-isopropylmalate mutant enzyme L106M, at pH 7.6 and 25°C Shewanella oneidensis
50.5
-
 NAD+ mutant enzyme L106M, at pH 7.6 and 25°C Shewanella oneidensis

Cofactor

Cofactor Comment Organism Structure
NAD+
-
 Shewanella benthica
NAD+
-
 Shewanella oneidensis

kcat/KM [mM/s]

kcat/KM Value [1/mMs-1] kcat/KM Value Maximum [1/mMs-1] Substrate Comment Organism Structure
60
-
 NAD+ mutant enzyme I268A, at pH 7.6 and 25°C Shewanella benthica
100
-
 NAD+ mutant enzyme A266S, at pH 7.6 and 25°C Shewanella benthica
180
-
 NAD+ wild type enzyme, at pH 7.6 and 25°C Shewanella benthica
190
-
 NAD+ mutant enzyme M106L, at pH 7.6 and 25°C Shewanella benthica
270
-
 (2R,3S)-3-isopropylmalate mutant enzyme I268A, at pH 7.6 and 25°C Shewanella benthica
590
-
 (2R,3S)-3-isopropylmalate mutant enzyme A266S, at pH 7.6 and 25°C Shewanella benthica
620
-
 (2R,3S)-3-isopropylmalate mutant enzyme M106L, at pH 7.6 and 25°C Shewanella benthica
1350
-
 (2R,3S)-3-isopropylmalate wild type enzyme, at pH 7.6 and 25°C Shewanella benthica