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Literature summary for 1.1.1.85 extracted from

  • Miyazaki, J.; Nakaya, S.; Suzuki, T.; Tamakoshi, M.; Oshima, T.; Yamagishi, A.
    Ancestral residues stabilizing 3-isopropylmalate dehydrogenase of an extreme thermophile: experimental evidence supporting the thermophilic common ancestor hypothesis (2001), J. Biochem., 129, 777-782.
    View publication on PubMed

Cloned(Commentary)

Cloned (Comment) Organism
wild type and mutant leuB genes are overexpressed in Escherichia coli Sulfurisphaera tokodaii

Protein Variants

Protein Variants Comment Organism
A259S/F261P Tm-value of mutant enzyme is 1.1°C higher than Tm-value of wild-type enzyme Sulfurisphaera tokodaii
G154A Tm-value of mutant enzyme is 1.9°C higher than Tm-value of wild-type enzyme Sulfurisphaera tokodaii
K152R Tm-value of mutant enzyme is 0.4°C lower than Tm-value of wild-type enzyme Sulfurisphaera tokodaii
K152R/G154A Tm-value of mutant enzyme is 1.2°C higher than Tm-value of wild-type enzyme Sulfurisphaera tokodaii
M91L/I95L Tm-value of mutant enzyme is 3.2°C higher than Tm-value of wild-type enzyme Sulfurisphaera tokodaii
M91L/I95L/K152R/G154A/A259S/F261P/Y282L Tm-value of mutant enzyme is 0.4°C higher than Tm-value of wild-type enzyme Sulfurisphaera tokodaii
Y282L Tm-value of mutant enzyme is 0.3°C lower than Tm-value of wild-type enzyme Sulfurisphaera tokodaii

Organism

Organism UniProt Comment Textmining
Sulfurisphaera tokodaii P50455
-
-
Sulfurisphaera tokodaii 7 P50455
-
-

Purification (Commentary)

Purification (Comment) Organism
-
Sulfurisphaera tokodaii

Synonyms

Synonyms Comment Organism
IPMDH
-
Sulfurisphaera tokodaii
leuB
-
Sulfurisphaera tokodaii

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
95
-
Tm-value of mutant enzyme Y282L is 95.7°C Sulfurisphaera tokodaii
96
-
Tm-value of wild-type enzyme is 96°C. Tm-value of mutant enzyme K152R is 95.6°C. Tm-value of mutant enzyme (M91L/I95L/K152R/G154A/A259S/F261P/Y282L) is 96.4°C Sulfurisphaera tokodaii
97
-
Tm-value of mutant enzyme A259S/F261P is 97.1°C Sulfurisphaera tokodaii
97
-
Tm-value of mutant enzyme K152R/G154A is 97.2°C Sulfurisphaera tokodaii
98
-
Tm-value of mutant enzyme G154A is 97.9°C Sulfurisphaera tokodaii
99
-
t1/2: 3.67 min (wild-type enzyme), 5.52 min (mutant enzyme M91L/I95L), 6.56 min (K152R/G154A mutant enzyme), 5.72 min (K152R mutant enzyme), 7.13 min (G154A mutant enzyme), 4.79 min (A259S/F261P mutant enzyme), 3.79 min (Y282L mutant enzyme), 6.5 min (M91L/I95L/K152R/G154A/A259S/F261P/Y282L mutant enzyme) Sulfurisphaera tokodaii
99
-
Tm-value of mutant enzyme M91L/I95L is 99.2°C Sulfurisphaera tokodaii

General Information

General Information Comment Organism
physiological function the enzyme is involved in the leucine biosynthetic pathway Sulfurisphaera tokodaii