Cloned (Comment) | Organism |
---|---|
wild type and mutant leuB genes are overexpressed in Escherichia coli | Sulfurisphaera tokodaii |
Protein Variants | Comment | Organism |
---|---|---|
A259S/F261P | Tm-value of mutant enzyme is 1.1°C higher than Tm-value of wild-type enzyme | Sulfurisphaera tokodaii |
G154A | Tm-value of mutant enzyme is 1.9°C higher than Tm-value of wild-type enzyme | Sulfurisphaera tokodaii |
K152R | Tm-value of mutant enzyme is 0.4°C lower than Tm-value of wild-type enzyme | Sulfurisphaera tokodaii |
K152R/G154A | Tm-value of mutant enzyme is 1.2°C higher than Tm-value of wild-type enzyme | Sulfurisphaera tokodaii |
M91L/I95L | Tm-value of mutant enzyme is 3.2°C higher than Tm-value of wild-type enzyme | Sulfurisphaera tokodaii |
M91L/I95L/K152R/G154A/A259S/F261P/Y282L | Tm-value of mutant enzyme is 0.4°C higher than Tm-value of wild-type enzyme | Sulfurisphaera tokodaii |
Y282L | Tm-value of mutant enzyme is 0.3°C lower than Tm-value of wild-type enzyme | Sulfurisphaera tokodaii |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Sulfurisphaera tokodaii | P50455 | - |
- |
Sulfurisphaera tokodaii 7 | P50455 | - |
- |
Purification (Comment) | Organism |
---|---|
- |
Sulfurisphaera tokodaii |
Synonyms | Comment | Organism |
---|---|---|
IPMDH | - |
Sulfurisphaera tokodaii |
leuB | - |
Sulfurisphaera tokodaii |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
95 | - |
Tm-value of mutant enzyme Y282L is 95.7°C | Sulfurisphaera tokodaii |
96 | - |
Tm-value of wild-type enzyme is 96°C. Tm-value of mutant enzyme K152R is 95.6°C. Tm-value of mutant enzyme (M91L/I95L/K152R/G154A/A259S/F261P/Y282L) is 96.4°C | Sulfurisphaera tokodaii |
97 | - |
Tm-value of mutant enzyme A259S/F261P is 97.1°C | Sulfurisphaera tokodaii |
97 | - |
Tm-value of mutant enzyme K152R/G154A is 97.2°C | Sulfurisphaera tokodaii |
98 | - |
Tm-value of mutant enzyme G154A is 97.9°C | Sulfurisphaera tokodaii |
99 | - |
t1/2: 3.67 min (wild-type enzyme), 5.52 min (mutant enzyme M91L/I95L), 6.56 min (K152R/G154A mutant enzyme), 5.72 min (K152R mutant enzyme), 7.13 min (G154A mutant enzyme), 4.79 min (A259S/F261P mutant enzyme), 3.79 min (Y282L mutant enzyme), 6.5 min (M91L/I95L/K152R/G154A/A259S/F261P/Y282L mutant enzyme) | Sulfurisphaera tokodaii |
99 | - |
Tm-value of mutant enzyme M91L/I95L is 99.2°C | Sulfurisphaera tokodaii |
General Information | Comment | Organism |
---|---|---|
physiological function | the enzyme is involved in the leucine biosynthetic pathway | Sulfurisphaera tokodaii |