Protein Variants | Comment | Organism |
---|---|---|
E87G | mutation does not affect the dissociation constant from the binary complex, but does greatly increase the KM-values | Thermus thermophilus |
E87Q | mutation does not affect the dissociation constant from the binary complex, but does greatly increase the KM-values | Thermus thermophilus |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
isocitrate | - |
Thermus thermophilus | |
NADH | NADH always produces competitive inhibition patterns with respect to NAD and noncompetitive inhibition patterns with respect to the substrates | Thermus thermophilus | |
Tartrate | - |
Thermus thermophilus |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.000017 | - |
isopropylmalate | pH 7.3, 21°C, cofactor: NAD+, wild-type enzyme | Thermus thermophilus | |
0.00048 | - |
isopropylmalate | pH 7.3, 21°C, cofactor: NAD+, mutant enzyme E87G | Thermus thermophilus | |
0.00298 | - |
isopropylmalate | pH 7.3, 21°C, cofactor: NADP+, wild-type enzyme | Thermus thermophilus | |
0.00321 | - |
NAD+ | pH 7.3, 21°C, reaction with isopropylmalate, wild-type enzyme | Thermus thermophilus | |
0.0053 | - |
isopropylmalate | pH 7.3, 21°C, cofactor: NAD+, mutant enzyme E87Q | Thermus thermophilus | |
0.0326 | - |
malate | pH 7.3, 21°C, cofactor: NAD+, isopropylmalate dehydrogenase mutant E87Q | Thermus thermophilus | |
0.0407 | - |
Tartrate | pH 7.3, 21°C, cofactor: NAD+, wild-type enzyme | Thermus thermophilus | |
0.0454 | - |
NAD+ | pH 7.3, 21°C, reaction with tartrate, wild-type enzym | Thermus thermophilus | |
0.1065 | - |
NAD+ | pH 7.3, 21°C, reaction with malate, wild-type enzyme | Thermus thermophilus | |
0.131 | - |
NAD+ | pH 7.3, 21°C, isopropylmalate dehydrogenase mutant E87G | Thermus thermophilus | |
0.181 | - |
NAD+ | pH 7.3, 21°C, isopropylmalate dehydrogenase mutant E87Q | Thermus thermophilus | |
0.386 | - |
NAD+ | pH 7.3, 21°C, isopropylmalate dehydrogenase mutant E87G | Thermus thermophilus | |
0.761 | - |
Tartrate | pH 7.3, 21°C, cofactor: NAD+, isopropylmalate dehydrogenase mutant E87Q | Thermus thermophilus | |
0.778 | - |
NAD+ | pH 7.3, 21°C, isopropylmalate dehydrogenase mutant E87Q | Thermus thermophilus | |
0.817 | - |
Tartrate | pH 7.3, 21°C, cofactor: NADP+, wild-type enzyme | Thermus thermophilus | |
1.21 | - |
Tartrate | pH 7.3, 21°C, cofactor: NAD+, mutant enzyme E87G | Thermus thermophilus | |
1.51 | - |
malate | pH 7.3, 21°C, cofactor: NAD+, wild-type enzyme | Thermus thermophilus | |
1.573 | - |
NADP+ | pH 7.3, 21°C, reaction with tartrate, wild-type enzyme | Thermus thermophilus | |
1.836 | - |
NADP+ | pH 7.3, 21°C, reaction with isopropylmalate, wild-type enzyme | Thermus thermophilus | |
1.853 | - |
NAD+ | pH 7.3, 21°C, reaction with malate, mutant enzyme E87Q | Thermus thermophilus | |
2.584 | - |
NAD+ | pH 7.3, 21°C, reaction with malate, mutant enzyme E87G | Thermus thermophilus | |
6.579 | - |
NADP+ | pH 7.3, 21°C, recation with malate, wild-type enzyme | Thermus thermophilus | |
31.56 | - |
malate | pH 7.3, 21°C, cofactor: NADP+, wild-type enzyme | Thermus thermophilus | |
35.59 | - |
malate | pH 7.3, 21°C, cofactor: NAD+, isopropylmalate dehydrogenase mutant E87G | Thermus thermophilus |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Thermus thermophilus | - |
- |
- |
Reaction | Comment | Organism | Reaction ID |
---|---|---|---|
(2R,3S)-3-isopropylmalate + NAD+ = (2S)-2-isopropyl-3-oxosuccinate + NADH + H+ | the oxidative decarboxylation of 2-hydroxy acid substrates by the enzyme occurs via a random kinetic mechanism with the formation of two abortive complexes: enzyme-NADH-substrate and enzyme-NADH-inhibitor | Thermus thermophilus |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
(2R,3S)-3-isopropylmalate + NAD+ | - |
Thermus thermophilus | 2-oxoisocaproate + NADH + H+ + CO2 | - |
? | |
malate + NAD+ | - |
Thermus thermophilus | pyruvate + NADH + H+ + CO2 | - |
? | |
malate + NADP+ | - |
Thermus thermophilus | pyruvate + NADPH + CO2 | - |
? | |
tartrate + NAD+ | - |
Thermus thermophilus | ? | - |
? | |
tartrate + NADP+ | - |
Thermus thermophilus | ? | - |
? |
Subunits | Comment | Organism |
---|---|---|
dimer | 2 * 35000-40000, SDS-PAGE | Thermus thermophilus |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.1 | - |
Tartrate | pH 7.3, 21°C, reaction with NADP+, wild-type enzyme | Thermus thermophilus | |
0.1 | - |
NADP+ | pH 7.3, 21°C, reaction with tartrate, wild-type enzyme | Thermus thermophilus | |
0.19 | - |
Tartrate | pH 7.3, 21°C, reaction with NAD+, wild-type enzyme | Thermus thermophilus | |
0.19 | - |
malate | pH 7.3, 21°C, reaction with NAD+, mutant enzyme E87Q | Thermus thermophilus | |
0.19 | - |
NAD+ | pH 7.3, 21°C, reaction with tartrate, wild-type enzyme or reaction with malate, mutant enzyme E87Q | Thermus thermophilus | |
0.2 | - |
isopropylmalate | pH 7.3, 21°C, reaction with NAD+, mutant enzyme E87G | Thermus thermophilus | |
0.2 | - |
NAD+ | pH 7.3, 21°C, reaction with isopropylmalate, mutant enzyme E87G | Thermus thermophilus | |
0.21 | - |
isopropylmalate | pH 7.3, 21°C, reaction with NAD+, mutant enzyme E87Q | Thermus thermophilus | |
0.21 | - |
Tartrate | pH 7.3, 21°C, reaction with NAD+, mutant enzyme E87G | Thermus thermophilus | |
0.21 | - |
NAD+ | pH 7.3, 21°C, reaction with tartrate, mutant enzyme E87G | Thermus thermophilus | |
0.21 | - |
NAD+ | pH 7.3, 21°C, reaction with isopropylmalate, mutant enzyme E87Q | Thermus thermophilus | |
0.24 | - |
isopropylmalate | pH 7.3, 21°C, reaction with NAD+, wild-type enzyme | Thermus thermophilus | |
0.24 | - |
NAD+ | pH 7.3, 21°C, reaction with isopropylmalate, wild-type enzyme | Thermus thermophilus | |
0.26 | - |
Tartrate | pH 7.3, 21°C, reaction with NAD+, mutant enzyme E87Q | Thermus thermophilus | |
0.26 | - |
malate | pH 7.3, 21°C, reaction with NAD+, mutant enzyme E87G | Thermus thermophilus | |
0.26 | - |
NAD+ | pH 7.3, 21°C, reaction with malate, mutant enzyme E87G or reaction with tartrate, mutant enzyme E87Q | Thermus thermophilus | |
1.66 | - |
isopropylmalate | pH 7.3, 21°C, reaction with NADP+, wild-type enzyme | Thermus thermophilus | |
1.66 | - |
NADP+ | pH 7.3, 21°C, reaction with isopropylmalate, wild-type enzyme | Thermus thermophilus | |
4.69 | - |
malate | pH 7.3, 21°C, reaction with NADP+, wild-type enzyme | Thermus thermophilus | |
4.69 | - |
NADP+ | pH 7.3, 21°C, reaction with malate, wild-type enzyme | Thermus thermophilus | |
10.6 | - |
malate | pH 7.3, 21°C, reaction with NAD+, wild-type enzyme | Thermus thermophilus | |
10.6 | - |
NAD+ | pH 7.3, 21°C, reaction with malate, wild-type enzyme | Thermus thermophilus |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
NAD+ | - |
Thermus thermophilus | |
NADP+ | - |
Thermus thermophilus |
Ki Value [mM] | Ki Value maximum [mM] | Inhibitor | Comment | Organism | Structure |
---|---|---|---|---|---|
additional information | - |
additional information | - |
Thermus thermophilus |