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Literature summary for 1.1.1.85 extracted from

  • Dean, A.M.; Dvorak, L.
    The role of glutamate 87 in the kinetic mechanism of Thermus thermophilus isopropylmalate dehydrogenase (1995), Protein Sci., 4, 2156-2167.
    View publication on PubMedView publication on EuropePMC

Protein Variants

Protein Variants Comment Organism
E87G mutation does not affect the dissociation constant from the binary complex, but does greatly increase the KM-values Thermus thermophilus
E87Q mutation does not affect the dissociation constant from the binary complex, but does greatly increase the KM-values Thermus thermophilus

Inhibitors

Inhibitors Comment Organism Structure
isocitrate
-
Thermus thermophilus
NADH NADH always produces competitive inhibition patterns with respect to NAD and noncompetitive inhibition patterns with respect to the substrates Thermus thermophilus
Tartrate
-
Thermus thermophilus

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.000017
-
isopropylmalate pH 7.3, 21°C, cofactor: NAD+, wild-type enzyme Thermus thermophilus
0.00048
-
isopropylmalate pH 7.3, 21°C, cofactor: NAD+, mutant enzyme E87G Thermus thermophilus
0.00298
-
isopropylmalate pH 7.3, 21°C, cofactor: NADP+, wild-type enzyme Thermus thermophilus
0.00321
-
NAD+ pH 7.3, 21°C, reaction with isopropylmalate, wild-type enzyme Thermus thermophilus
0.0053
-
isopropylmalate pH 7.3, 21°C, cofactor: NAD+, mutant enzyme E87Q Thermus thermophilus
0.0326
-
malate pH 7.3, 21°C, cofactor: NAD+, isopropylmalate dehydrogenase mutant E87Q Thermus thermophilus
0.0407
-
Tartrate pH 7.3, 21°C, cofactor: NAD+, wild-type enzyme Thermus thermophilus
0.0454
-
NAD+ pH 7.3, 21°C, reaction with tartrate, wild-type enzym Thermus thermophilus
0.1065
-
NAD+ pH 7.3, 21°C, reaction with malate, wild-type enzyme Thermus thermophilus
0.131
-
NAD+ pH 7.3, 21°C, isopropylmalate dehydrogenase mutant E87G Thermus thermophilus
0.181
-
NAD+ pH 7.3, 21°C, isopropylmalate dehydrogenase mutant E87Q Thermus thermophilus
0.386
-
NAD+ pH 7.3, 21°C, isopropylmalate dehydrogenase mutant E87G Thermus thermophilus
0.761
-
Tartrate pH 7.3, 21°C, cofactor: NAD+, isopropylmalate dehydrogenase mutant E87Q Thermus thermophilus
0.778
-
NAD+ pH 7.3, 21°C, isopropylmalate dehydrogenase mutant E87Q Thermus thermophilus
0.817
-
Tartrate pH 7.3, 21°C, cofactor: NADP+, wild-type enzyme Thermus thermophilus
1.21
-
Tartrate pH 7.3, 21°C, cofactor: NAD+, mutant enzyme E87G Thermus thermophilus
1.51
-
malate pH 7.3, 21°C, cofactor: NAD+, wild-type enzyme Thermus thermophilus
1.573
-
NADP+ pH 7.3, 21°C, reaction with tartrate, wild-type enzyme Thermus thermophilus
1.836
-
NADP+ pH 7.3, 21°C, reaction with isopropylmalate, wild-type enzyme Thermus thermophilus
1.853
-
NAD+ pH 7.3, 21°C, reaction with malate, mutant enzyme E87Q Thermus thermophilus
2.584
-
NAD+ pH 7.3, 21°C, reaction with malate, mutant enzyme E87G Thermus thermophilus
6.579
-
NADP+ pH 7.3, 21°C, recation with malate, wild-type enzyme Thermus thermophilus
31.56
-
malate pH 7.3, 21°C, cofactor: NADP+, wild-type enzyme Thermus thermophilus
35.59
-
malate pH 7.3, 21°C, cofactor: NAD+, isopropylmalate dehydrogenase mutant E87G Thermus thermophilus

Organism

Organism UniProt Comment Textmining
Thermus thermophilus
-
-
-

Reaction

Reaction Comment Organism Reaction ID
(2R,3S)-3-isopropylmalate + NAD+ = (2S)-2-isopropyl-3-oxosuccinate + NADH + H+ the oxidative decarboxylation of 2-hydroxy acid substrates by the enzyme occurs via a random kinetic mechanism with the formation of two abortive complexes: enzyme-NADH-substrate and enzyme-NADH-inhibitor Thermus thermophilus

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
(2R,3S)-3-isopropylmalate + NAD+
-
Thermus thermophilus 2-oxoisocaproate + NADH + H+ + CO2
-
?
malate + NAD+
-
Thermus thermophilus pyruvate + NADH + H+ + CO2
-
?
malate + NADP+
-
Thermus thermophilus pyruvate + NADPH + CO2
-
?
tartrate + NAD+
-
Thermus thermophilus ?
-
?
tartrate + NADP+
-
Thermus thermophilus ?
-
?

Subunits

Subunits Comment Organism
dimer 2 * 35000-40000, SDS-PAGE Thermus thermophilus

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
0.1
-
Tartrate pH 7.3, 21°C, reaction with NADP+, wild-type enzyme Thermus thermophilus
0.1
-
NADP+ pH 7.3, 21°C, reaction with tartrate, wild-type enzyme Thermus thermophilus
0.19
-
Tartrate pH 7.3, 21°C, reaction with NAD+, wild-type enzyme Thermus thermophilus
0.19
-
malate pH 7.3, 21°C, reaction with NAD+, mutant enzyme E87Q Thermus thermophilus
0.19
-
NAD+ pH 7.3, 21°C, reaction with tartrate, wild-type enzyme or reaction with malate, mutant enzyme E87Q Thermus thermophilus
0.2
-
isopropylmalate pH 7.3, 21°C, reaction with NAD+, mutant enzyme E87G Thermus thermophilus
0.2
-
NAD+ pH 7.3, 21°C, reaction with isopropylmalate, mutant enzyme E87G Thermus thermophilus
0.21
-
isopropylmalate pH 7.3, 21°C, reaction with NAD+, mutant enzyme E87Q Thermus thermophilus
0.21
-
Tartrate pH 7.3, 21°C, reaction with NAD+, mutant enzyme E87G Thermus thermophilus
0.21
-
NAD+ pH 7.3, 21°C, reaction with tartrate, mutant enzyme E87G Thermus thermophilus
0.21
-
NAD+ pH 7.3, 21°C, reaction with isopropylmalate, mutant enzyme E87Q Thermus thermophilus
0.24
-
isopropylmalate pH 7.3, 21°C, reaction with NAD+, wild-type enzyme Thermus thermophilus
0.24
-
NAD+ pH 7.3, 21°C, reaction with isopropylmalate, wild-type enzyme Thermus thermophilus
0.26
-
Tartrate pH 7.3, 21°C, reaction with NAD+, mutant enzyme E87Q Thermus thermophilus
0.26
-
malate pH 7.3, 21°C, reaction with NAD+, mutant enzyme E87G Thermus thermophilus
0.26
-
NAD+ pH 7.3, 21°C, reaction with malate, mutant enzyme E87G or reaction with tartrate, mutant enzyme E87Q Thermus thermophilus
1.66
-
isopropylmalate pH 7.3, 21°C, reaction with NADP+, wild-type enzyme Thermus thermophilus
1.66
-
NADP+ pH 7.3, 21°C, reaction with isopropylmalate, wild-type enzyme Thermus thermophilus
4.69
-
malate pH 7.3, 21°C, reaction with NADP+, wild-type enzyme Thermus thermophilus
4.69
-
NADP+ pH 7.3, 21°C, reaction with malate, wild-type enzyme Thermus thermophilus
10.6
-
malate pH 7.3, 21°C, reaction with NAD+, wild-type enzyme Thermus thermophilus
10.6
-
NAD+ pH 7.3, 21°C, reaction with malate, wild-type enzyme Thermus thermophilus

Cofactor

Cofactor Comment Organism Structure
NAD+
-
Thermus thermophilus
NADP+
-
Thermus thermophilus

Ki Value [mM]

Ki Value [mM] Ki Value maximum [mM] Inhibitor Comment Organism Structure
additional information
-
additional information
-
Thermus thermophilus