Activating Compound | Comment | Organism | Structure |
---|---|---|---|
thioredoxin | activated by thioredoxin m that is reduced either photochemically with ferredoxin and ferredoxin-thioredoxin reductase or chemically with dithiothreitol | Zea mays |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
50000 | 60000 | unactivated enzyme, gel filtration | Zea mays |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Zea mays | - |
- |
- |
Oxidation Stability | Organism |
---|---|
the enzyme undergoes reversible oxidation/reduction during its photoregulation | Zea mays |
Purification (Comment) | Organism |
---|---|
- |
Zea mays |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
leaf | - |
Zea mays | - |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
160 | - |
- |
Zea mays |
Storage Stability | Organism |
---|---|
-15°C, 37.5 mM Na-acetate, pH 5.5, 0.37 mM EDTA, 25% glycerol, no significant loss of activity after 3 months | Zea mays |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
oxaloacetate + NADH | utilization of NADH to NADPH in reduction of oxaloacetate is 1:160 | Zea mays | (S)-malate + NAD+ | - |
? | |
oxaloacetate + NADPH | - |
Zea mays | (S)-malate + NADP+ | - |
? |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
7 | 8.5 | activated enzyme | Zea mays |