Activating Compound | Comment | Organism | Structure |
---|---|---|---|
guanidine | can activate oxidized enzyme | Pisum sativum |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
HgCl2 | activity of the oxidized enzyme in presence of guanidine is unaffected by 0.1 mM HgCl2 in presence of 1 mM EDTA and by 0.005 mM HgCl2 in absence of EDTA, activity of the reduced form of enzyme is completely abolished | Pisum sativum | |
NEM | 1 mM, activity of the oxidized enzyme in presence of guanidine is unaffected, activity of the reduced form of enzyme is completely abolished | Pisum sativum |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.039 | - |
NADPH | reduced native enzyme | Pisum sativum | |
0.048 | - |
oxaloacetate | reduced native enzyme | Pisum sativum | |
0.077 | - |
oxaloacetate | reduced enzyme, + 200 mM guanidine | Pisum sativum | |
0.095 | - |
NADPH | reduced enzyme, + 200 mM guanidine | Pisum sativum | |
0.22 | - |
NADPH | oxidized enzyme, + 250 mM guanidine | Pisum sativum | |
0.32 | - |
oxaloacetate | oxidized enzyme, + 250 mM guanidine | Pisum sativum |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Pisum sativum | - |
- |
- |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
leaf | - |
Pisum sativum | - |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
oxaloacetate + NADPH | - |
Pisum sativum | (S)-malate + NADP+ | - |
? |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
8 | - |
guanidine-activated oxidized enzyme and reduced enzyme | Pisum sativum |