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Literature summary for 1.1.1.81 extracted from
- Purification and characterization of a novel NADPH(NADH)-dependent hydroxypyruvate reductase from spinach leaves. Comparison of immunological properties of leaf hydroxypyruvate reductases (1988), Biochem. J., 250, 145-152.
Inhibitors
| Inhibitors | Comment | Organism | Structure |
|---|---|---|---|
| additional information | not inhibited by 2 mM acetohydroxamate | Spinacia oleracea |
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.8 | - |
Hydroxypyruvate | at pH 6.0 | Spinacia oleracea |  |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 38000 | - |
2 * 38000, SDS-PAGE | Spinacia oleracea |
| 70000 | - |
gel filtration | Spinacia oleracea |
Natural Substrates/ Products (Substrates)
| Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| hydroxypyruvate + NAD(P)H | Spinacia oleracea | - |
D-glycerate + NAD(P)+ + H+ | - |
? | |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Spinacia oleracea | - |
- |
- |
Source Tissue
| Source Tissue | Comment | Organism | Textmining |
|---|---|---|---|
| leaf | - |
Spinacia oleracea | - |
Specific Activity [micromol/min/mg]
| Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|
| 257 | - |
- |
Spinacia oleracea |
Storage Stability
| Storage Stability | Organism |
|---|---|
| -20°C, 20 mM MOPS, pH 7.1, 14 mM 2-mercaptoethanol, 50% glycerol | Spinacia oleracea |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| glyoxylate + NAD(P)H | activity with hydroxypyruvate and NADPH is 2fold higher than with other pair of reactants | Spinacia oleracea | glycolate + NAD(P)+ | - |
ir | |
| hydroxypyruvate + NAD(P)H | - |
Spinacia oleracea | D-glycerate + NAD(P)+ + H+ | - |
? | |
| hydroxypyruvate + NAD(P)H | - |
Spinacia oleracea | D-glycerate + NAD(P)+ + H+ | - |
ir | |
Subunits
| Subunits | Comment | Organism |
|---|---|---|
| dimer | 2 * 38000, SDS-PAGE | Spinacia oleracea |
pH Range
| pH Minimum | pH Maximum | Comment | Organism |
|---|---|---|---|
| 5.5 | 6 | strong decrease above | Spinacia oleracea |
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Release 2023.1 (January 2023)
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